Intravenous adipose-derived mesenchymal stem cell therapy for the treatment of feline asthma: a pilot study

Trzil, Julie E; Masseau, Isabelle; Webb, Tracy L.; Chang, Chee‐Hoon; Dodam, John R.; Liu, Hong; Quimby, Jessica M.; Dow, Steven; Reinero, Carol R.

doi:10.1177/1098612x15604351

Cited by 52 publications

(52 citation statements)

References 35 publications

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“…These results suggest that multiple treatments with MSCs may be required to help manage chronic disease. The findings in an experimentally induced acute asthma trial were similar in that airway remodeling in MSC-treated cats (intravenous infusion of allogeneic MSCs) was significantly decreased compared to controls (85). There was also a decrease in airway eosinophilia and hyper-responsiveness in MSC treated cats compared to controls.…”

Section: Respiratory System and Mscsmentioning

confidence: 53%

“…Intravenous injection of allogeneic MSCs in cats with chronic kidney disease has produced complications that include vomiting and increased respiratory rate and effort (135). In other feline studies, five infusions of allogeneic MSCs from multiple donors was tolerated with no complications (85). Preactivated allogeneic canine MSCs (2 × 10 6 cells/kg body weight) have been administered intravenously every 2 weeks for three doses without complications (64).…”

Section: Safety and Limitationsmentioning

confidence: 99%

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The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses

MacDonald

Barrett

2020

Front. Vet. Sci.

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Section: Respiratory System and Mscsmentioning

confidence: 53%

Section: Safety and Limitationsmentioning

confidence: 99%

The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses

MacDonald

Barrett

2020

Front. Vet. Sci.

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“…Beyond humans, there are numerous clinical applications for MSCs in the veterinary field, including the treatment of feline diseases such as gingivostomatitis, inflammatory bowel disease, allergic asthma and chronic kidney disease [7][8][9][10]. Research on MSC-based treatment for feline ailments is expanding our understanding of the potential of stem cells, along with the challenges associated with feline cells.…”

Section: Introductionmentioning

confidence: 99%

Viability, Yield and Expansion Capability of Feline MSCs Obtained from Subcutaneous and Reproductive Organ Adipose Depots

Wysong

Ortiz

Bittel

et al. 2020

Preprint

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Background: The source of multipotent stromal cells (MSC) can have a significant influence on the health and expansion capacity of the cells. As the applications for allogeneic MSCs in the treatment of feline diseases increase, the location of the initial donor tissue must be analyzed. To date, comparisons have only been made between feline MSCs collected from bone marrow or abdominal fat. This is the first report to compare cells obtained from different adipose depots in the cat. The adipose tissue was collected from 32 healthy cats undergoing spaying (fat around the ovaries and uterine horn) or subcutaneous fat collected during surgical procedures. Results: The total tissue yield from the subcutaneous fat was significantly greater than could be obtained from around the reproductive organs, leading to 3 times more total MSCs. However, the density of MSCs obtained from reproductive fat was significantly higher than from subcutaneous fat. In addition, the viability of the MSCs from the reproductive fat was significantly higher than the subcutaneous fat. When sufficient tissue was collected, it was digested either mechanically or enzymatically. Mechanical digestion further decreased the viability and yield of MSCs from subcutaneous fat compared to enzymatic digestion. Biomarkers of stem cell expansion and function were detected using qPCR. Gata6 was detected in all samples similarly regardless of site of harvest or method of digestion. Sox2 and Sox17 were also detected in all samples with higher quantities found in the enzymatically digested subcutaneous fat. Control genes of Gata4 and Pdx1 were detected in very low levels. Negative controls showed no detection prior to 50 cycles. During the first passage there was no statistically significant difference in doubling times or viability. But at P2, MSCs from the enzymatically-digested reproductive fat had the lowest doubling time and a trend towards the highest viability. Conclusion: Feline reproductive adipose tissue is a reasonable source of MSCs for eventual therapeutic applications with superior cell density, viability and expansion

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“…Cell therapy is a promising alternative for the treatment of several pathologies in animal species. For instance, adipose tissue derived from mesenchymal stem cells (AMSCs) has been successfully applied to experimentally treat domestic cats affected by chronic kidney disease, asthma and gingivostomatitis (Quimby et al 2015, Trzil et al 2015, Arzi et al 2016. This treatment was also used to restore elbow functionality in the cougar (Puma concolor) (Gómez et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Differentiation and multipotential characteristics of mesenchymal stem cells derived from adipose tissue of an endangered wild cat (Leopardus guigna)

Echeverry

Rojas

Aguilera

et al. 2019

Austral j. vet. sci.

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Adipose tissue derived mesenchymal stem cells (AMSCs) had been isolated and used for cell therapy in domestic cats. For wild cats, the isolation of AMSCs has only been reported in the black-footed cat (Felis nigripes). AMSCs obtained from wild cats may be useful to treat injuries of endangered cat species that remain in captivity or arrive at wildlife rehabilitation centers. Additionally, AMSCs might allow improvement of cloning techniques or assist in derivation of induced pluripotent stem cells. Endangered wild cats such as the guigna (Leopardus guigna), an endemic and endangered species from Chile and Argentina, might benefit greatly from the development of novel treatments or techniques that can be applied for its conservation. The objective of this study was to characterise putative AMSCs from guigna in terms of their main biological attributes, particularly, growth kinetics, differentiation ability and surface marker expression. Results obtained from this characterisation were compared with AMSCs isolated from domestic cats. AMSCs were isolated from peritoneal adipose tissue of female cats and subcutaneous tissue from a female guigna. Migration potential, colony-forming unit assay, mesodermal differentiation and surface marker expression (CD45, CD44, CD90, MHCI and MHCII) were evaluated. Domestic cat and guigna AMSCs displayed similar growth properties in culture. Both AMSC types showed mesodermal differentiation potential, in vitro homing potential and similar surface marker expression. These results indicate that AMSCs from subcutaneous tissue of guigna could have potential use as regenerative treatment for this species and might be considered for use in other biotechnological applications.

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Intravenous adipose-derived mesenchymal stem cell therapy for the treatment of feline asthma: a pilot study

Abstract: MSCs may have a delayed effect in reducing airway inflammation, airway hyper-responsiveness and remodeling in experimentally induced asthmatic cats. Results warrant additional investigation of MSC therapy for asthma in cats.

Cited by 52 publications

References 35 publications

The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses

The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses

Viability, Yield and Expansion Capability of Feline MSCs Obtained from Subcutaneous and Reproductive Organ Adipose Depots

Differentiation and multipotential characteristics of mesenchymal stem cells derived from adipose tissue of an endangered wild cat (Leopardus guigna)

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