2009
DOI: 10.1186/1475-2867-9-6
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Intrinsic anticarcinogenic effects of Piper sarmentosum ethanolic extract on a human hepatoma cell line

Abstract: Background: Piper sarmentosum, locally known as kaduk is belonging to the family of Piperaceae. It is our interest to evaluate their effect on human hepatoma cell line (HepG2) for the potential of anticarcinogenic activity.

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Cited by 68 publications
(35 citation statements)
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“…Formation of DNA fragmentation is one of the characteristic features observed in apoptotic cells and it is generally considered as the biochemical hallmark of apoptosis [36]. The formation of a DNA ladder correlates with the early morphological signs of apoptosis and has been widely used as a distinctive marker of the apoptosis process [37]. The ladder-like appearance of DNA observed in the HepG2 cells treated with CD-3, was also another indicator of the apoptosis inducing capability of the CD-3.…”
Section: Discussionmentioning
confidence: 99%
“…Formation of DNA fragmentation is one of the characteristic features observed in apoptotic cells and it is generally considered as the biochemical hallmark of apoptosis [36]. The formation of a DNA ladder correlates with the early morphological signs of apoptosis and has been widely used as a distinctive marker of the apoptosis process [37]. The ladder-like appearance of DNA observed in the HepG2 cells treated with CD-3, was also another indicator of the apoptosis inducing capability of the CD-3.…”
Section: Discussionmentioning
confidence: 99%
“…Three independent cell counts (counting a minimum of 200 total cells) were obtained on the basis of differential staining of nuclei. Acridine orange is taken up by both live and dead cells and emits green fluorescent, whereas ethidium bromide stains only dead cells which lost their membrane integrity and emits red fluorescent [20]. …”
Section: Methodsmentioning
confidence: 99%
“…Plate was washed with PBS three times and 400 μL of acridine orange (0.01% in PBS pH‐7.4) was added and incubated at 37 °C for 20 min. The plate was washed thrice with PBS and observed under the fluorescent microscope for any nuclear morphological changes (9).…”
Section: Methodsmentioning
confidence: 99%