Intrinsic enzyme mimicking activity of gold nanoclusters upon visible light triggering and its application for colorimetric trypsin detection

Enzyme Mimics: Advances and Applications

“…Colourimetric biosensors depend on visual observations or spectrometric measurements of colourc hanges associated with bioanalytes.T hey have the advantages of low cost, simplicity,a nd portability and, in some cases, are instrument-free, for example, pregnancy test strips. Many colorimetric biosensors employing enzyme mimics rely on the catalytic oxidation of peroxidase substrates, such as tetramethylbenzidine( TMB), in the presenceo fH 2 O 2 .B ased on this process, colorimetric biosensors for trypsin, [227] DNA, [228] thrombin [229] and glucose [71,[230][231][232][233][234] have been developed with enzymem imics such as gold nanoclusters, [227] carboxyl-modified graphene oxide, [71] Fe 3 O 4, [229,230] CuS, [230] and Co x Fe 3Àx O 4 [232] NPs. For example, Yan and co-workers discovered that biologically and chemically inert Fe 3 O 4 magnetic NPs exhibit peroxidase-like activity.…”

Gold nanoclusters-based chemiluminescence resonance energy transfer method for sensitive and label-free detection of trypsin

“…Au NCs can be stabilized by the steric of the bulkiness of BSA [32]. When BSA templates were hydrolyzed by trypsin, the structure of Au NCs would be destroyed [30,31]. The CL signal of the system was significantly decreased when adding 1.0 mg mL À 1 trypsin into above CL system.…”

“…As illustrating in Scheme 1, the reaction between TCPO and H 2 O 2 generates energy-rich intermediate 1,2-dioxetanedione, which transfers the energy to BSA-stabilized Au NCs and produces intense CL signal. BSA molecule can be cleft by trypsin [30,31]. When BSA molecule was degraded, the molecule structure of Au NCs was destroyed.…”

Resonance Rayleigh Scattering and SERS Spectral Detection of Trace Hg(II) Based on the Gold Nanocatalysis

“…Since Fe 3 O 4 nanoparticles have been found to have intrinsic peroxidase-like activity [3], nanoenzyme have become interesting to people. They have vast application prospects with advantages of easy production process, good stability and recycling, low-cost of storage and transport, and high adaptability of heat, acid, and alkali [1,2,3,4,5,6,7,8,9,10,11]. It is a new study filed, that is, how to organically combine nanoenzyme catalysis with its physical and chemical properties to create more novel functions.…”

“…It is a new study filed, that is, how to organically combine nanoenzyme catalysis with its physical and chemical properties to create more novel functions. In analytical chemistry, nanoenzymes have been used in absorption, fluorescence, and chemiluminescence analysis [3,4,5,6,7,8,9,10,11,12,13]. Gold nanoparticles have peroxidase activity that catalyze the colored redox reaction of 3,3′,5,5′-tetramethylbenzidine (TMB)-H 2 O 2 [12].…”