Introduction of New Computer Softwares for Classification and Prediction Purposes of Bioactive Peptides: Case Study in Antioxidative Tripeptides

Abstract

Antioxidative tripeptides were successfully classified according to their structure by using Sequence Principal Component Similarity (SPCS) software. During SPCS computation, hydrophobicity was the main characteristic of peptide residues in aspect of antioxidative activity to inhibit lipid peroxidation in linoleic acid model system. The prediction made by Homology Similarity Search (HSS)-BIOPEP combined software indicated that C-hordein fraction from barley protein showed the greatest potential to be antioxidative protein source followed by prolamin fraction from rice protein. Moreover, these homology segments of C-hordein were resistant against the digestion of mixed gastrointestinal enzymes in BIOPEP digestive model system.

Partial Characterization of Ultrafiltrated Soy Protein Hydrolysates with Antioxidant and Free Radical Scavenging Activities

“…Although this characteristic was negatively correlated ( P < 0.001) to FRSA, a closer look at the hydrophobicity and FRSA of ultrafiltrated fractions (Table and , respectively) within each hydrolysate makes noticeable a trend of a higher surface hydrophobicity as the MWCO decreased (significant only for C3), which may be related to the enhanced ability of low‐MW fractions to scavenge DPPH. The importance of hydrophobicity on the ability of small peptide fractions to trap free radicals has also been described by Chanput and others () and Tang and others ().…”

“…Park and others () purified a potent antioxidant peptide fraction, from an Alcalase soy hydrolysate, with a considerably high amount of Phe (55%). This amino acid has also been identified in several sequences of antioxidant peptides purified from other protein sources (Rajapakse and others ; Chanput and other ; Chen and others ). Antioxidant activity of Phe can be attributed to the susceptibility of its aromatic ring to oxidation (Udenigwe and Aluko ).…”

“…The most antioxidant peptide fractions in this study had a MWCO <3 kDa (F3 and C3). Several peptide fractions within this molecular size range have been reported to have the abilty to decrease lipid oxidation in model systems (Chanput and others ; Park and others ; Ajibola and others ). According to Kong and Xiong (), small peptides can easily diffuse to the water–oil interface in the liposome or emulsion system where oxidation occurs.…”

Antioxidant Properties of Tripeptides Revealed by a Comparison of Six Different Assays

“…The Quantitative Structure-Activity Relationships (QSAR) concept has been extended to antioxidant peptides by two research groups (Chanput et al, 2010;Li et al, 2011a;Li et al, 2011b). They used the same experimental results reported by Saito et al (2003); i.e.…”

“…The one research group indicated that hydrophobic amino acids such as Ile, Leu, Phe, Trp, Tyr, and Val were required for antioxidant peptide segments (Chanput et al, 2010). The other research group showed that the N-terminal amino acid should be a high hydrophobic and low electronic amino acid (such as Ala, Gly, Val, and Leu); the center amino acid would be an amino acid that possesses a high hydrogen bond property (such as basic amino acids Arg, Lys, and His) (Li et al, 2011a;Li et al, 2011b).…”