Introgression of A- and B-genome of tetraploid triticale chromatin into tetraploid rye

Abstract

An improvement of rye is one of the mainstream goals of current breeding. Our study is concerned with the introduction of the tetraploid triticale (ABRR) into the 4x rye (RRRR) using classical methods of distant crossing. One hundred fifty BC1F9 hybrid plants [(4x rye × 4x triticales) × 4x rye] obtained from a backcrossing program were studied. The major aim of this work was to verify the presence of an introgressed A- and B- genome chromatin of triticale in a collection of the 4x rye-tiritcale hybrids and to determine their chromosome compositions. In the present study, karyotypes of the previously reported BC1F2s and BC1F3s were compared with that of the BC1F9 generation as obtained after several subsequent open pollinations. The genomic in situ hybridisation (GISH) allowed us to identify 133 introgression forms in which chromosome numbers ranged between 26 and 32. Using four DNA probes (5S rDNA, 25S rDNA, pSc119.2 and pAs1), the fluorescence in situ hybridisation (FISH) was carried out to facilitate an exact chromosome identification in the hybrid plants. The combination of the multi-colour GISH with the repetitive DNA FISH singled out five types of translocated chromosomes: 2A.2R, 4A.4R, 5A.5R, 5B.5R and 7A.7R among the examined BC1F9s. The reported translocation lines could serve as valuable sources of wheat chromatin suitable for further improvements of rye.

Similarities and differences in the nuclear genome organization within Pooideae species revealed by comparative genomic in situ hybridization (GISH)

“…The occurrence of signals detected in phylogenetically distant species may indicate strong relationships between repetitive sequences in selected grasses. For example, the pSc119.2 sequence from rye is widely used in the identification of wheat chromosomes and its relatives (Cuadrado and Jouve 1994 ; Schneider et al 2003 , 2005 ; Wiśniewska et al 2013 ).…”

Transmission of the Aegilops ovata chromosomes carrying gametocidal factors in hexaploid triticale (×Triticosecale Wittm.) hybrids

“…Image processing was carried out using Olympus Cell-F (version 3.1; Olympus Soft Imaging Solutions GmbH, Münster, Germany) imaging software and PaintShop Pro X5 software (version 15.0.0.183; Corel Corporation, Ottawa, Canada). The identification of particular chromosomes was made by comparing the signal pattern of 5S rDNA, 25S rDNA, pSc119.2 and pAs1 probes according to a previous study (Kwiatek et al 2013 ) and similar cytogenetic analysis (Cuadrado and Jouve 1994 ; Schneider et al 2003 , 2005 ; Wiśniewska et al 2013 ).…”

Characterization of morphology and resistance to Blumeria graminis of winter triticale monosomic addition lines with chromosome 2D of Aegilops tauschii

“…Image processing was carried out using the Olympus Cell-F (version 3.1; Olympus Soft Imaging Solutions GmbH: Münster, Germany) imaging software and PaintShop Pro X5 software (version 15.0.0.183; Corel Corporation, Ottawa, Canada). The identification of particular chromosomes was made by comparing the signal pattern of selected probes (5S rDNA, 25S rDNA, pSc119.2, and pAs1) according to a previous study (Kwiatek et al 2013 , 2015 ) and similar cytogenetic analysis (Cuadrado and Jouve 1994 ; Schneider et al 2003 , 2005 ; Wiśniewska et al 2013 ).…”