Inulin-Ethylenediamine Coated SPIONs Magnetoplexes: A Promising Tool for Improving siRNA Delivery

Licciardi, Mariano; Volsi, Anna Li; Sardo, Carla; Mauro, Nicolò; Cavallaro, Gennara; Giammona, Gaetano

doi:10.1007/s11095-015-1726-y

Cited by 25 publications

(20 citation statements)

References 27 publications

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“…Another polysaccharide used recently in siRNA delivery is inulin. This polysaccharide made up of fructose and glucose units is usually processed with oligoamines such as ethylenediamine123 or diethylenetriamine and imidazole124,125 in order to generate cationic groups for siRNA binding and therefore favor endosomal escape.…”

Section: Delivery Systemsmentioning

confidence: 99%

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

Chalbatani¹,

Dana²,

Gharagouzloo³

et al. 2019

IJN

188

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Cancer is one of the most complex diseases that has resulted in multiple genetic disorders and cellular abnormalities. Globally, cancer is the most common health concern disease that is affecting human beings. Great efforts have been made over the past decades in biology with the aim of searching novel and more efficient tools in therapy. Thus, small interfering RNAs (siRNAs) have been considered one of the most noteworthy developments which are able to regulate gene expression following a process known as RNA interference (RNAi). RNAi is a post-transcriptional mechanism that involves the inhibition of gene expression through promoting cleavage on a specific area of a target messenger RNA (mRNA). This technology has shown promising therapeutic results for a good number of diseases, especially in cancer. However, siRNA therapeutics have to face important drawbacks in therapy including stability and successful siRNA delivery in vivo. In this regard, the development of effective siRNA delivery systems has helped addressing these issues by opening novel therapeutic windows which have allowed to build up important advances in Nanomedicine. In this review, we discuss the progress of siRNA therapy as well as its medical application via nanoparticle-mediated delivery for cancer treatment.

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Section: Delivery Systemsmentioning

confidence: 99%

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

Chalbatani¹,

Dana²,

Gharagouzloo³

et al. 2019

IJN

188

View full text Add to dashboard Cite

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“… 12 Furthermore, an amino inulin derivative has been used to coat SPIONs yielding stable magnetoplexes by complexation of inulin coated SPIONs with a model duplexed siRNA, for improving oligonucleotide transfection efficiency. 13 The ability to amino-modulate inulin renders it an attractive candidate for the functionalization of gold nanoparticles, taking advantage of the well documented thiol and amino chemistry at gold surfaces. 14 We propose here the use of PEG as a pre-stabilizing agent to avoid undesired nanoparticle aggregation, 15 , 16 whilst the amino-derived inulin based copolymer is the main biocompatible coating agent for the 40 nm gold nanospheres.…”

Section: Introductionmentioning

confidence: 99%

Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy

et al. 2016

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“…Moreover coated GNPs should guarantee a prolonged blood circulation time and provide active functional groups useful to conjugate targeting ligands to GNPs surface. Our group has synthetized many polymeric materials that may be used to coat metallic nanoparticles, including synthetic polyamino acid derivatives [16] and polysaccharides based constructs [17,18]. Among them, inulin, a natural, biocompatible, and biodegradable polysaccharide consisting of linear chains of -(2-1) fructose units that exhibit many hydroxyl functional groups that make inulin versatile and easy to functionalize, has been employed as coating agent for metal NPs and used in several biomedical applications [17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Inulin Coated Gold Nanoparticles for Selective Delivery of Doxorubicin to Breast Cancer Cells

Licciardi

Volsi

Mauro

et al. 2016

Journal of Nanomaterials

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A novel folate-targeted gold-based nanosystem for achieving selectivity towards folate receptor (FR) positive cells is proposed, by virtue of the fact that the FR is a molecularly targeted entity overexpressed in a wide spectrum of solid tumors. A new inulin-folate derivative (INU-FA) has been synthesized to act as coating agent for 40 nm gold nanoparticles. The obtained polymer-coated gold nanoparticles (Au@INU-FA) were characterized in terms of hydrodynamic radius, shape, zeta potential, and aqueous stability and were loaded with doxorubicin (Au@INU-FA/Doxo). Its release capability was tested in different release media. The selectivity of Au@INU-FA/Doxo system towards FRs-positive cancer cells was proved by the differences in the quantitative uptake using human breast cancer MCF7 as FR-positive cells and 16HBE epithelial as noncancer cell line. Furthermore, the folate-mediated uptake mechanism was studied by FRs-blocking experiments. On the whole Au@INU-FA/Doxo was able to be preferentially internalized into MCF7 cells proving a folate-mediated endocytosis mechanism which allowed a higher and selective cytotoxic effect towards cancer cells. The cytotoxicity profile was evaluated on both cancer and noncancer cell lines, displaying that folate-mediated targeting implied advantageous therapeutic effects, such as amplified drug uptake and increased anticancer activity towards MCF7 cancer cells.

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Inulin-Ethylenediamine Coated SPIONs Magnetoplexes: A Promising Tool for Improving siRNA Delivery

Abstract: These findings support the potential application of this system as a magnetically targeted drug delivery system. Graphical Abstract Magnetically improved siRNA transfection in cells under magnetofective conditions upon uptake enhancement of IC-SPIONs in the presence of an external magnet.

Cited by 25 publications

References 27 publications

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy

Preparation and Characterization of Inulin Coated Gold Nanoparticles for Selective Delivery of Doxorubicin to Breast Cancer Cells

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