Invariant Natural Killer T Cells Play a Role in Chemotaxis, Complement Activation and Mucus Production in a Mouse Model of Airway Hyperreactivity and Inflammation

Karisola, Piia; Lehto, Maili; Kinaret, Pia Anneli Sofia; Ahonen, Niina; Haapakoski, Rita; Anthoni, Minna; Taniguchi, Masaru; Wolff, Henrik; Puustinen, Anne; Alenius, Harri

doi:10.1371/journal.pone.0129446

Cited by 3 publications

(1 citation statement)

References 44 publications

(48 reference statements)

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“…The duet CCR2/CCL2 was reduced in control mice vs. OVA-sensitized mice in a study that evaluated the expression of CD1d, an MHC-1 like molecule, responsible for presenting glycolipids to iTCR and iNKT cells conducting exacerbate airway inflammation and up-regulating IgE production ( 123 ). Along with other proteins, CXCL15 contributed to iNKT regulated AHR via altering leukocyte chemotaxis in a murine study ( 124 ). Data from animal studies (murine) demonstrate that iNKT cell-mediated XCL1-XCR1 axis promotes AHR by recruiting CD103 + DCs into the lung in patients with allergic asthma ( 125 ).…”

Section: Chemokinesmentioning

confidence: 99%

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review)

et al. 2020

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Allergic diseases have been classified in the last decades using various theories. The main classes of the newest classification in allergic respiratory diseases focus on the characterization of the endotype (which takes into account biomarkers related to determinant pathophysiological mechanisms) and of the phenotype (based on the description of the disease). Th2, Th1 and Th17 lymphocytes and the type of inflammatory response mediated by them represent the basis for Th2 and non-Th2 endotype classification. In addition, new lymphocytes were also used to characterize allergic diseases: Th9 lymphocytes, Th22 lymphocytes, T follicular helper cells (T FH) lymphocytes and invariant natural killer T (iNKT) lymphocytes. In the last decade, a growing body of evidence focused on chemokines, chemoattractant cytokines, which seems to have an important contribution to the pathogenesis of this pathology. This review presents the interactions between chemokines and Th lymphocytes in the context of Th2/non-Th2 endotype classification of respiratory allergies.

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Section: Chemokinesmentioning

confidence: 99%

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review)

et al. 2020

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NKT cells contribute to basal IL-4 production but are not required to induce experimental asthma

et al. 2017

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CD1d-deficiency results in a selective deletion of NKT cells in mice that is reported to prevent murine allergic airway disease (AAD). Because we find 2–3 fold lower basal IL-4 production in CD1d- mice than in wild-type (WT) mice, we hypothesized that the contribution made by NKT cells to AAD would depend on the strength of the stimulus used to induce the disease. Consequently, we compared CD1d-deficient mice to WT mice in the development of AAD, using several models of disease induction that differed in the type and dose of allergen, the site of sensitization and the duration of immunization. Surprisingly we found equivalent allergic inflammation and airway disease in WT and CD1d- mice in all models investigated. Consistent with this, NKT cells constituted only ~2% of CD4+ T cells in the lungs of mice with AAD, and IL-4-transcribing NKT cells did not expand with disease induction. Concerned that the congenital absence of NKT cells might have caused a compensatory shift within the immune response, we administered an anti-CD1d monoclonal Ab (mAb) to block NKT function before airway treatments, before or after systemic sensitization to antigen. Such Ab treatment did not affect disease severity. We suggest that the differences reported in the literature regarding the significance of NKT cells in the induction of allergic airway disease may have less to do with the methods used to study the disease and more to do with the animals themselves and/or the facilities used to house them.

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Intra-tracheal Administration of <em>Haemophilus influenzae</em> in Mouse Models to Study Airway Inflammation

Venuprasad

Theivanthiran

Cantarel

2016

JoVE

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Citation: Venuprasad, K., Theivanthiran, B., Cantarel, B. Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (109), e53964, doi:10.3791/53964 (2016). AbstractHere, we describe a detailed procedure to efficiently and directly deliver Haemophilus influenzae into the lower respiratory tracts of mice. We demonstrate the procedure for preparing H. influenzae inoculum, intra-tracheal instillation of H. influenzae into the lung, collection of bronchoalveolar lavage fluid (BALF), analysis of immune cells in the BALF, and RNA isolation for differential gene expression analysis. This procedure can be used to study the lung inflammatory response to any bacteria, virus or fungi. Direct tracheal instillation is mostly preferred over intranasal or aerosol inhalation procedures because it more efficiently delivers the bacterial inoculum into the lower respiratory tract with less ambiguity. Video LinkThe video component of this article can be found at

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Invariant Natural Killer T Cells Play a Role in Chemotaxis, Complement Activation and Mucus Production in a Mouse Model of Airway Hyperreactivity and Inflammation

Cited by 3 publications

References 44 publications

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review)

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review)

NKT cells contribute to basal IL-4 production but are not required to induce experimental asthma

Intra-tracheal Administration of <em>Haemophilus influenzae</em> in Mouse Models to Study Airway Inflammation

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