Invertase in Cell Walls of Plant Tissue Cultures

Straus, Jacob

doi:10.1104/pp.37.3.342

Cited by 57 publications

(18 citation statements)

References 19 publications

(12 reference statements)

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“…Invertase I activity continues to rise during incubation preceding the period of greatest growth (i.e., cell elongation), and it then declines. In the absence of exogenous substrate and hormone, cell division activity within the excised intercalary meristem rapidly declines (12 (15), in contrast to other tissues where the bulk invertase activity is associated with the cell wall fraction (6,16,28). Invertase activity could not be removed from the cell wall fraction by the procedures tested.…”

Section: Methodsmentioning

confidence: 83%

Multiple Forms of Invertase in Developing Oat Internodes

Jones¹,

Kaufman²

1975

Plant Physiol.

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Section: Methodsmentioning

confidence: 83%

Multiple Forms of Invertase in Developing Oat Internodes

Jones¹,

Kaufman²

1975

Plant Physiol.

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“…Invertase activity has been reported to be present in the soluble and insoluble (cell wall) fractiotis (Straus, 1962;Straus & Campbell, 1963;Copping & Street, 1971;Krishnan, Blanchette & Okita, 1985). Our preliminary studies showed that the orchid invertase was also present in the cell wall fraction.…”

Section: Discussionmentioning

confidence: 99%

“…In tbe orcbid culture medium containing sucrose as tbe sole carbon source, tbere was an increase in glucose concentration witb a concomitant decrease in total carbohydrate, niie same bad been reported for some otber plant tissues in culture, and invertase activity was implicated (Straus, 1962;Straus & Campbell, 1963;Coppling & Street, 1972;Thorpe & Meier, 1973). To date, tbere is no report of invertase activity in orchid tissue culture under culture conditions.…”

Section: N T K O D L' C T [ O Nmentioning

confidence: 99%

“…The isolation of invertase was carried out according to the method of Straus (1962) and Schaffer (1986), with some modifications. The tissues were homogenized for 3 min in 15 ml of 25 mM HEPESNaOH buffer (pH 7.2) containing 0-5 mM EDTA, 5 mM MgCl.^, 3 mM dithiothreitol, 2 mM diethyldithiocarbamate and 2% polyvinylpyrollidone in an Ultra-Turrax TP18/12 homogenizer (JankeKunkel).…”

Section: Isolation and Assay Of Invertase Activitymentioning

confidence: 99%

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Sugar uptake and invertase activity in Dendrobium tissues

Hew

Mah

1989

New Phytologist

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S U M M .'\ R YLJndifferentiiited and differentiated tissues ot" Dcudrobitim exhibited no difference in their preference for sugars. The uptake of sugars was more rapid by undifferentiated tissue. I-'ructose provided the best carbon source for botli tissues. Sucrose was hydrolysed before it was taken up, as the result of invertase acti\ ity. Both acid and alkaline invertase were present and invertase activity was detected in the soluble and cell wall traction. There was a gratlual increase in invertase activity of tissues grown in sucrose-containing medium with time but not in tissues grown in fructose-containing medium.

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“…Reports of the activities of sucrose-degrading enzymes in plant tissue cultures (3,14,15), for example, vary in the degree of, correspondence observed between enzyme activity and growth rate. Growth rate may be limited by substrate concentration as well as by enzyme concentration, and a simple correspondence between enzyme activity and growth is unlikely to exist throughout a culture period during which the carbohydrate supply in the medium may become completely exhausted (16).…”

Section: Sucrosementioning

confidence: 99%

Carbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus

Hunt

Loomis²

1976

Plant Physiol.

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Logistic curves were fitted to sigmoidal growth data obtained from tobacco (Nicotiana rustica L.) callus grown on media prepared with 0.1, 0.03, 0.01, and 0.003 M sucrose. Analysis of the growth curves indicated that final yields and specific growth rates were influenced by the initial sucrose concentration. Growth yields from the four treatments were similar (0. MATERIALS AND METHODSTobacco callus cultures grown on Linsmaier and Skoog's medium (10) were used following preliminary tests with a large number of cultivars of four species on four basic media with a range of hormonal concentrations. The Linsmaier and Skoog medium was developed with a view towards optimizing medium requirements for growth (10, 11) and, with tobacco, gave us the highest growth rates of relatively undifferentiated callus.The callus was generated from the culture of internodal pith explants from greenhouse-grown tobacco plants (Nicotiana rustica L.). After several subcultures on Linsmaier and Skoog's medium (10) in darkness at 27 C, three vigorously growing pieces were dissected for inoculum.Ninety-six, 125-ml Erlenmeyer flasks containing 40 ml of Linsmaier and Skoog's medium were prepared for inoculation with four treatment sucrose concentrations (0.1, 0.03, 0.01, and 0.003 M). Cytokinin was added at a nonlimiting concentration (0.93 uM kinetin). All flasks were inoculated with four evenly spaced pieces of tobacco callus (3 mm3 per piece). The callus was firm to cut but disintegrated easily. Loose tissue was not used as inoculum as the size could not be estimated. The flasks were incubated in darkness at 27 C.Fresh and dry weight measurements were made of 20 pieces of inoculum (collectively) and of the callus from six randomly selected flasks for each treatment on four sampling dates: 7, 28, 43, and 55 days after inoculation. Dry weights were determined after drying for 24 hr in a Thermo-Vac RD-6 freeze-dryer. RESULTSThe growth of tobacco callus for the four sucrose supply treatments is illustrated in Figure 1. Yields followed a sigmoidal pattern with time and weighted logistic growth curves were fitted to the data by an iterative program that minimized the residual variance of the data points from the curve.

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Invertase in Cell Walls of Plant Tissue Cultures

Cited by 57 publications

References 19 publications

Multiple Forms of Invertase in Developing Oat Internodes

Multiple Forms of Invertase in Developing Oat Internodes

Sugar uptake and invertase activity in Dendrobium tissues

Carbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus

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