Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model

Adipose mesenchymal stem cells in the field of bone tissue engineering

“…A clear trend has emerged toward the use of composite scaffolds due to their superior properties and structures [80][81][82] derived from the combination of two or more materials [83][84][85][86][87] . The study of hASCs for bone regeneration has largely involved the insertion of biomaterials in rat and nude mouse models [88][89][90][91][92] . Furthermore, a femoral defect in nude rats is available and calvarial defect models have been described for other species, to demonstrate the application and optimization of hASCs in regenerative medicine [93][94][95][96][97] .…”

Phenotypic differences during the osteogenic differentiation of single cell-derived clones isolated from human lipoaspirates

“…The osteogenic potential of hASCs has been further supported by preliminary studies using different scaffold materials in animal models (Hicok et al , 2004). Additionally, it has been demonstrated that the treatment of these cells in the presence of inductive factors enhanced the osteogenic differentiation of subcutaneously implanted cells (Justesen et al , 2004; Leong et al , 2006). The observed differentiation of hASCs towards the possible precursors of neurogenic (Safford et al , 2002) or hepatic (Seo et al , 2005) lineages under specific conditions suggests a plasticity of these cells which could prove to be of more interest in regenerative medicine (Helder et al , 2007).…”

Characterization of novel akermanite:poly-ϵ-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering

“…Of the adult pluripotent cell sources used in scaffold assisted‐bone strategies, human adipose‐derived stem cells (hASC) are of particular interest (Lendeckel et al ., ; Gaalen et al ., ) since they provide a readily autologous adult stem cell source (Gaalen et al ., ; Gimble et al ., ) available through minimally invasive surgical procedures (Gaalen et al ., ). Additionally, hASC osteogenesis has been recently investigated in a variety of bone‐substitute scaffolds (Hattori et al ., ; Leong et al ., ; Scherberich et al ., ; Dégano et al ., ; Jeon et al ., ; Liu et al ., ; De Girolamo et al ., ; Haimi et al ., ; McCullen and Zhu, ; Zhang et al ., ; Frohlich et al ., ; Gastaldi et al ., ; Marino et al ., ; Muller and Mehrkens, ; Wang et al ., ; Lee and Lee, ; Weinand et al ., ). While clinical trials have successfully demonstrated the bone growth and tissue repair benefits of scaffold‐hASC in humans (Lendeckel et al ., ; Mesimäki et al ., ; Gimble et al ., ; Thesleff et al ., ), further investigations using scaffolds with improved osteogenic capacity are still needed.…”

“…Ideal scaffold materials for future hASC bone tissue engineering strategies should be biocompatible, absorbable, easy to handle and capable of supporting hASC growth and differentiation while providing predictable mechanical and degradation properties during the healing process (Reichert and Hutmacher, ). Among the materials currently used in synthetic hASC bone tissue engineering strategies, β‐tricalcium phosphate (β‐TCP) is the most common (Leong et al ., ; Liu et al ., ; Marino et al ., ; McCullen and Zhu, ; Zhang et al ., ; Wang et al ., ; Weinand et al ., ). β‐TCP is a biocompatible synthetic apatite Ca 3 (PO 4 ) 2 (Gaalen et al ., ), and when used as scaffolding material, β‐TCP has been demonstrated under certain experimental conditions to have osteoconductive and osteoinductive properties superior to natural bone hydroxyapatite (HA) (Gaalen et al ., ; Muller and Mehrkens, ; Wang et al ., ).…”