Investigating the role of the thiol‐regulated enzyme sedoheptulose‐1,7‐bisphosphatase in the control of photosynthesis

Raines, Christine A.; Harrison, Elizabeth; Ölçer, Hülya; Lloyd, Julie C.

doi:10.1111/j.1399-3054.2000.1100303.x

Cited by 23 publications

(25 citation statements)

References 51 publications

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“…The motif CGGTAC existing in redox-active groups of a number of enzymes has also been observed in SlSBPase. Cys116 and Cys121 in this motif are predicted to be involved in redox-regulated activation of tomato SBPase as observed in wheat26, rice27, cucumber28 and Arabidopsis 15.…”

Section: Resultsmentioning

confidence: 96%

Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants

Ding

Wang

Zhang

et al. 2016

Sci Rep

118

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Sedoheptulose-1, 7-bisphosphatase (SBPase) is an important enzyme involved in photosynthetic carbon fixation in the Calvin cycle. Here, we report the impact of changes in SBPase activity on photosynthesis, growth and development, and chilling tolerance in SBPase antisense and sense transgenic tomato (Solanum lycopersicum) plants. In transgenic plants with increased SBPase activity, photosynthetic rates were increased and in parallel an increase in sucrose and starch accumulation was evident. Total biomass and leaf area were increased in SBPase sense plants, while they were reduced in SBPase antisense plants compared with equivalent wild-type tomato plants. Under chilling stress, when compared with plants with decreased SBPase activity, tomato plants with increased SBPase activity were found to be more chilling tolerant as indicated by reduced electrolyte leakage, increased photosynthetic capacity, and elevated RuBP regeneration rate and quantum efficiency of photosystem II. Collectively, our data suggest that higher level of SBPase activity gives an advantage to photosynthesis, growth and chilling tolerance in tomato plants. This work also provides a case study that an individual enzyme in the Calvin cycle may serve as a useful target for genetic engineering to improve production and stress tolerance in crops.

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Section: Resultsmentioning

confidence: 96%

Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants

Ding

Wang

Zhang

et al. 2016

Sci Rep

118

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“…For example, RuBisCO activity is controlled by proton levels and magnesium ions (Tapia et al 2000, Andersson 2008). Fructose-1,6-bisphosphatase, seduheptulose-1,7-bisphosphatase (SBPase) and Phosphoribulokinase are all controlled by the redox state through the thioredoxin-ferredoxin system, and also by pH (Chiadmi et al 1999, Raines et al 2000, Raines 2003). Furthermore, Hendriks et al showed the light dependency of the ADP-glucose pyrophosphorylase (Hendriks et al 2003), which is part of the lumped reaction v Starch in our model.…”

Section: Resultsmentioning

confidence: 99%

“…This allows for a systematic variation of the energy demand by simulating accelerated or decelerated carbon fixation activity. Performing this variation under different light conditions gives insight into the synchronization of ATP and NADPH production and consumption rates, and thus enables a more profound analysis of the supply-demand regulation of photosynthesis (Brandes et al 1996, Chiadmi et al 1999, Raines et al 2000. For the following steady-state analysis, the conceptual Ru5P influx reaction is not included.…”

Section: Resultsmentioning

confidence: 99%

Balancing energy supply during photosynthesis – a theoretical perspective

Matuszyńska

Saadat

Ebenhöh

2019

Physiologia Plantarum

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The photosynthetic electron transport chain (PETC) provides energy and redox equivalents for carbon fixation by the Calvin‐Benson‐Bassham (CBB) cycle. Both of these processes have been thoroughly investigated and the underlying molecular mechanisms are well known. However, it is far from understood by which mechanisms it is ensured that energy and redox supply by photosynthesis matches the demand of the downstream processes. Here, we deliver a theoretical analysis to quantitatively study the supply–demand regulation in photosynthesis. For this, we connect two previously developed models, one describing the PETC, originally developed to study non‐photochemical quenching, and one providing a dynamic description of the photosynthetic carbon fixation in C3 plants, the CBB Cycle. The merged model explains how a tight regulation of supply and demand reactions leads to efficient carbon fixation. The model further illustrates that a stand‐by mode is necessary in the dark to ensure that the carbon fixation cycle can be restarted after dark–light transitions, and it supports hypotheses, which reactions are responsible to generate such mode in vivo.

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“…Sedoheptulose 1,7-bisphosphatase (SBPase; EC 3.1.3.37) catalyzes the dephosphorylation of sedoheptulose-1,7-bisphosphate in the regenerative phase of the Calvin-Benson cycle. Such a substrate-specific SBPase is found only in oxygenic photosynthetic eukaryotes and is unique to the Calvin-Benson cycle (220,221).…”

Section: (59)mentioning

confidence: 99%