Investigation and Application Progress of Vero Cell Serum-free Culture

Chen, Tian; Chen, Keping

doi:10.5539/ijb.v1n2p41

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…The culture medium is the most direct and dominant environmental factor that affects in vitro cell metabolism, proliferation and survival (14). The general culture conditions for EC/ES cells (15) Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation include a high-glucose formulation medium containing animal serum in the form of fetal calf serum (FCS)/fetal bovine serum (FBS).…”

Section: Introductionmentioning

confidence: 99%

“…It is a source of nutrients, hormones, vitamins, growth factors and protease inhibitors that enable the survival and proliferation of cells in vitro. It also facilitates the attachment and spreading of cells, provides non-specific protection against mechanical damage, and it is able to bind to toxic compounds (14,17). However, besides these positive properties, serum has some major disadvantages.…”

Section: Introductionmentioning

confidence: 99%

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Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

et al. 2015

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Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

et al. 2015

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History of Vaccine Process Development

Pujar

Sagar

Lee³

2014

Vaccine Development and Manufacturing

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The Effect of Non Freeze-dried Hydrogel-CHA on Fibroblast Proliferation

Wahyudi

Nurwadji

2015

J Dent Indones

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Bone damage can be caused by variety of surgical procedures. Bone reconstruction has been developed lately is tissue engineering techniques. One of materials that proved to be effective as a scaffold in tissue engineering is a hydrogel. The addition of carbonate apatite (CHA) will produce a hydrogel-CHA material which is believed to improve the mechanical properties and biological similarities with the original bone. Scaffold is considered an important aspect in the field of tissue engineering, because it's ability to mimic extracellular matrix of the damaged tissue. Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelial-mesenchymal interactions, secreting various growth factors and cytokines. On certain condition, Fibroblast will differentiate into bone-forming cells, osteoblasts. Objective: to determine the effect of non freezedried hydrogels -CHA on the number of fibroblasts. Methods: In the treatment groups (hydrogel and hydrogel-CHA group), the static seeding, where cells and scaffolds were simply brought into contact, was performed. The other group contained only cells and growth media. Cells were seeded at a density of 2x10 4 cells/ml in a 96-well plate. Number of fibroblasts cell in each group was observed by light microscopy and quantitified by MTT assay on days 1, 2 and 3 post-application. Results: Proliferation of fibroblasts increased significantly on day 3 rd after application of non freeze-dried hydrogel -CHA (p< 0.05). Conclusion: Application of non freeze-dried hydrogel -CHA may induce fibroblasts proliferation. ABSTRAKPengaruh aplikasi non freeze-dried hidrogel-CHA terhadap prolifrasi fibroblas. Kerusakan tulang dapat disebabkan oleh berbagai prosedur bedah. Rekonstruksi tulang yang banyak dikembangkan akhir-akhir ini adalah teknik rekayasa jaringan. Material yang terbukti efektif sebagai perancah dalam rekayasa jaringan adalah hidrogel. Penambahan Karbonat apatit (CHA) akan menghasilkan material hidrogel-CHA yang diyakini dapat meningkatkan sifat mekanis dan kemiripan biologis dengan tulang. Perancah merupakan aspek penting dalam bidang rekayasa jaringan, karena kemampuannya menyerupai matriks ekstraseluler pada jaringan yang rusak. Fibroblas merupakan sel mesenkim yang dengan mudah dibiakkan di laboratorium dan memiliki peran penting dalam interaksi epitel-mesenkimal, mensekresi berbagai faktor pertumbuhan dan sitokin. Pada kondisi tertentu fibroblas akan berdiferensiasi menjadi sel pembentuk tulang yaitu osteoblas. Tujuan: untuk mengetahui pengaruh non freeze-dried hidrogel-CHA terhadap jumlah sel fibroblas. Metode: Pada kelompok perlakuan (kelompok hidrogel dan hidrogel-CHA), penyemaian statis yaitu sel dan perancah dikontakkan. Pada kelompok lain hanya berisi sel dan media pertumbuhan. Sel disemai dengan kepadatan 2x10 4 sel/ml dalam 96 sumuran. Jumlah sel fibroblas dalam tiap kelompok diamati dengan menggunakan mikroskop dan dihitung dengan uji MTT pada hari 1, 2,dan 3 setelah aplikasi perlakuan. Hasil: Proliferasi...

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Investigation and Application Progress of Vero Cell Serum-free Culture

Cited by 4 publications

References 16 publications

Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

History of Vaccine Process Development

The Effect of Non Freeze-dried Hydrogel-CHA on Fibroblast Proliferation

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