Investigation into antibacterial and wound healing properties of platelets lysate against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections

Abstract

Background and aim Treatment of burn wound infections has become a global challenge due to the spread of multidrug-resistant bacteria; therefore, the development of new treatment options for the mentioned infections is essential. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections. Methods PdB was prepared through the freezing and thawing process and then, in vitro antibacterial effect was determined by disk diffusion and broth microdilution methods. Afterward, burn wound was inflicted on 56 rats, infected with both bacteria, and topical administration was performed to evaluate antibacterial effects and wound healing properties of PdB. Results In vitro results showed that PdB inhibited the growth of A. baumannii in the highest dose (0.5), while we did not detect any inhibitory effects against K. pneumoniae. By contrast, PdB significantly inhibited the growth of bacteria in treated animal wounds compared to the control groups (P value < 0.05). Macroscopic assessments pointed to the significant enhancement of wound closure in the treated animals. In addition, histopathological examination demonstrated that treatment of rats with PdB led to a considerable increase in re-epithelialization and attenuated the formation of granulation tissue (P value < 0.05). Conclusion The use of topical PdB is an attractive strategy for treating A. baumannii and K. pneumoniae burn wound infections because it inhibits bacterial growth and promotes wound healing properties.

A relative comparison of antibacterial strength between platelet lysate and platelet factor 4 in vitro

“…PdB could inhibit the growth of A. baumannii at the highest concentration (0.5), whereas no inhibitory effects were found against K. pneumoniae. In contrast, PdB could signi cantly inhibit the bacterial growth in treated animal wounds than the control groups (P value < 0.05) (33).…”

Antimicrobial Effects of Equine Platelet Lysate

“…An “extract liquid” obtained from activation and centrifugation of PRP was found to have a longer-lasting effect against S. aureus than activated PRP over 24 h ( 23 ). In other studies, activated PRP was shown to be effective against bacteria that are known to cause wound infections such as S. aureus and E. coli ( 20 , 24 , 25 ) but not against K. pneumonia, E. faecalis , or P. aeruginosa ( 24 , 26 ). Two independent studies showed that activated PRP had antibacterial effects against S. aureus , but not E. coli or Pseudomonas in human dermal ulcers ( 27 ) and in a rabbit spinal infection model ( 28 ).…”

Advances in antimicrobial hydrogels for dental tissue engineering: regenerative strategies for endodontics and periodontics

“…The selective antimicrobial action of the platelet lysates for specific types of bacteria was reported. 88 However, the antimicrobial mechanism of action of the platelet lysates has not been completely determined yet.…”