Investigation of association between IL-8 serum levels and IL8 polymorphisms in Chinese patients with sepsis

Hu, Donghai; Wang, Haiyan; Huang, Xia; Jiang, Yujie; Qin, Yueqiu; Xiong, Bin; Qin, Gang; Sooranna, Suren R.; Pinhu, Liao

doi:10.1016/j.gene.2016.09.024

Cited by 18 publications

(17 citation statements)

References 39 publications

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“…Interestingly enough, we also found a correlation between the genetic polymorphism and the serum CXCL8 level in this study. Similar results were found in Chinese patients with sepsis and the different genotypes within three SNPs are more susceptible to sepsis (Hu et al 2016). In another study involving a Chinese Holstein population, CXCL8 gene polymorphisms significantly correlated with the somatic cell score trait and different genotypes had significantly different mRNA expression levels (Chen et al 2015).…”

Section: Association Analysis Of the Porcine Cxcl8 Genesupporting

confidence: 72%

“…Akiba et al (2001) found that the serum CXCL8 level was markedly elevated in most patients with hepatocellular carcinoma compared with healthy subjects and a high serum CXCL8 level significantly correlated with a more aggressive tumour behaviour in patients with resectable hepatocellular carcinoma. The serum CXCL8 protein level was significantly higher in septic patients than in healthy patients (Hu et al 2016). Similarly, the serum CXCL8 level in individuals with a Helicobacter pylori infection also associated strongly with a degree of gastritis (Siregar et al 2015).…”

Section: Association Analysis Of the Porcine Cxcl8 Genementioning

confidence: 87%

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A single nucleotide polymorphism of the porcine CXCL8 gene is associated with serum CXCL8 level

Wang

Zhu

et al. 2019

Italian Journal of Animal Science

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2019) A single nucleotide polymorphism of the porcine CXCL8 gene is associated with serum CXCL8 levelABSTRACT C-X-C motif chemokine ligand 8 (CXCL8) gene, a crucial cytokine with roles in the immune system, functions in the inflammatory response by promoting the activation and migration of neutrophils. Previous studies have reported that the CXCL8 gene resides within the quantitative trait locus (QTL). In this report, the porcine CXCL8 gene was selected to investigate its effect on serum immune traits. CXCL8 mRNA expression was also detected by quantitative real-time polymerase chain reaction (PCR). A SNP (NC_010450.4:g.69934997T > C, rs81218904) of the CXCL8 gene was identified by direct nucleotide sequencing and the SNP was genotyped by MALDI-TOF MS in the three pig populations, which consisted of 300 piglets distributed in three pig breeds, Landrace (68 piglets), Large White (158 piglets) and Songliao Black (74 piglets). An indepth analysis indicated that there was a significant correlation between the SNP and the serum CXCL8 level (20 days) (14 days after vaccination) (p < .05). Our results suggest that the CXCL8 gene may be served as a genetic marker with effects on serum CXCL8 level in the pig disease resistance breeding. HIGHLIGHTSGenomic variant identification and tissues expression analysis of porcine CXCL8 gene. The SNP in porcine CXCL8 gene was significantly associated with serum CXCL8 level in three pig populations. SNP genotyping and association analysis results provided significant evidence for association on serum cytokine trait of porcine CXCL8 gene. ARTICLE HISTORY

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Section: Association Analysis Of the Porcine Cxcl8 Genesupporting

confidence: 72%

Section: Association Analysis Of the Porcine Cxcl8 Genementioning

confidence: 87%

A single nucleotide polymorphism of the porcine CXCL8 gene is associated with serum CXCL8 level

Wang

Zhu

et al. 2019

Italian Journal of Animal Science

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“…Elevated levels of IL-8 in fluids from the respiratory environment are closely associated with the neutrophilic asthma phenotype, among other inflammatory diseases (Simpson et al, 2008). However, it is also noted that IL-8 polymorphisms have been associated with differential IL-8 gene expression and disease [e.g., - sepsis (Hu et al, 2016), viral bronchiolitis (Pinto et al, 2016)]. In the case of asthma, Esposito et al (2014a) have previously reported an association between an IL-8 polymorphism and increased odds of wheezing among infants.…”

Section: Discussionmentioning

confidence: 99%

Whole Blood Cytokine Response to Local Traffic-Related Particulate Matter in Peruvian Children With and Without Asthma

et al. 2017

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This study sought to investigate if acute phase immune responses of whole blood from Peruvian children with controlled and uncontrolled asthma differed from children without asthma, following exposure to traffic-related particulate matter (TRPM). TRPM, including particulate matter from diesel combustion, has been shown to stimulate acute airway inflammation in individuals with and without asthma. For this study, a whole blood assay (WBA) was used to test peripheral whole blood samples from 27 children with asthma, and 12 without asthma. Participant blood samples were stimulated, ex vivo, for 24-h with an aqueous extract of TRPM that was collected near study area highways in Lima, Peru. All participant blood samples were tested against the same TRPM extract, in addition to purified bacterial endotoxin and pyrogen-free water, which served as positive and negative WBA controls, respectively. The innate and adaptive cytokine responses were evaluated in cell-free supernatants of the whole blood incubations. Comparatively similar levels were recorded for nine out of the 10 cytokines measured [e.g., – Interleukin (IL)-1β, IL-6, IL-10], regardless of study participant asthma status. However, IL-8 levels in TRPM-stimulated blood from children with uncontrolled asthma were diminished, compared to subjects without asthma (633 pg/ml vs. 1,023 pg/ml, respectively; p < 0.01); IL-8 responses for subjects with controlled asthma were also reduced, but to a lesser degree (799 pg/ml vs. 1,023 pg/ml, respectively; p = 0.10). These relationships were present before, and after, adjusting for age, sex, obesity/overweight status, C-reactive protein levels, and residential proximity to the study area’s major roadway. For tests conducted with endotoxin, there were no discernible differences in cytokine response between groups, for all cytokines measured. The WBA testing conducted for this study highlighted the capacity of the TRPM extract to potently elicit the release of IL-8 from the human whole blood system. Although the small sample size of the study limits the capacity to draw definitive conclusions, the IL-8 responses suggest that that asthma control may be associated with the regulation of a key mediator in neutrophil chemotaxis, at a systemic level, following exposure to PM derived from traffic-related sources.

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“…The heterozygote AT genotype has been associated with increased risk of developing severe sepsis [197]. In a more recent study, the male population carrying the homozygote TT genotype was found to be more susceptible to sepsis, while no association was determined between the 251A/T allele and IL-8 serum levels in septic patients [198].…”

Section: Il-8mentioning

confidence: 96%

“…Toll-like receptors (TLRs) -D299G allele of TLR4 gene associated with increased susceptibility to severe bacterial infections and Gram-negative sepsis [192,193] IL-8 -The 251A/T allele has been associated with survival [194], protection against sepsis [195], and also increased risk of sepsis [196] -The heterozygote AT genotype has been associated with increased risk of developing sepsis [197] -Male T allele carriers are more susceptible to sepsis [198] TNF-α -G-to-A polymorphism associated with sepsis [201] -G-to-A polymorphism associated with adverse outcomes in patients with severe sepsis and septic shock [199,200] -No association of G-to-A polymorphism and development of sepsis [202,203] -G-to-A polymorphism not associated with sepsis mortality [201] IL-6 -174 G/C polymorphism showed modest association with neonatal sepsis [209] -174 G/C polymorphism was associated with improved survival rates in patients with sepsis [207] -174 G/C polymorphism was not associated with a difference in survival [208] Angiotensin-converting enzyme (ACE)…”

Section: Diagnostic Significance Prognostic Significancementioning

confidence: 99%

Clinical Assays in Sepsis: Prognosis, Diagnosis, Outcomes, and the Genetic Basis of Sepsis

Vassiliou¹,

Orfanos²,

AnastasiaKotanidou³

2017

Sepsis

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Sepsis is the most widespread medical disorder of the intensive care unit (ICU) and the most common cause of death in hospitalized patients. Several endothelium-related molecules have been investigated as potential biomarkers for early diagnosis and/or prognosis of sepsis, providing different results depending on study designs. Therefore, it seems that we are still far from the right combination of sepsis markers to be used in clinical practice. It is more probable that a panel of diverse biomarkers will be more efficient in clinical practice. More recently, the potential use of genetic biomarkers for prognostic purposes started emerging for sepsis, in the form of genome-wide association studies. The successful use of modern molecular diagnostics could enable rapid identification of particularly susceptible or less susceptible individuals, leading to tailored therapeutic treatments.

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Investigation of association between IL-8 serum levels and IL8 polymorphisms in Chinese patients with sepsis

Cited by 18 publications

References 39 publications

A single nucleotide polymorphism of the porcine CXCL8 gene is associated with serum CXCL8 level

A single nucleotide polymorphism of the porcine CXCL8 gene is associated with serum CXCL8 level

Whole Blood Cytokine Response to Local Traffic-Related Particulate Matter in Peruvian Children With and Without Asthma

Clinical Assays in Sepsis: Prognosis, Diagnosis, Outcomes, and the Genetic Basis of Sepsis

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