Investigation of Biogenic Amines in Dried Bonito Flakes from Different Countries Using High-Performance Liquid Chromatography

The bacterial diversity of salted mackerel “one‐night courtyard” at soft frozen area (−7℃–0℃) storage was studied. The fish samples at 0, 14, 21, 28, and 35 days were analysis for bacterial structure using high‐throughput sequencing technologies (HTS) and biogenic amines using high‐performance liquid chromatography (HPLC). The analysis results of HTS showed that the dominant bacteria species was varied gradually following with storage time. On the 0th, 21st, and 28th days of storage, dominant Vibrionaceae was accounting for 71.70%, 59.16%, and 70.68% of the total sequences analyzed, respectively. On the 14th and 35th days, Shewanellaceae was the dominant bacterial, accounting for 87.53% and 70.95% of the total sequences analyzed, respectively. In addition, 21st and 28th days, an abundance of Operational Taxonomic Units (OTUs) was top. The dominant bacterial of Proteobacteria, Firmicutes, was producer of biogenic amines. Furthermore, the analysis results of HPLC shown the total biogenic amines of maximum amount 363.01 mg/kg in the sample of HY.14 lower than 1000 mg/kg of the FDA regulation. The range ability of cadaverine was obvious following with the storage time. Cadaverine was 87.36 mg/kg on the 0th day, and it was maximum amount of 276.89 mg/kg on the 14th days. Putrescine was 20 mg/kg on the 0th day and maximum amount of 55.04 mg/kg on the 28thdays of storage. The tyramine was smallest amount of production, and the largest amount was 38.99 mg/kg on 28th, and the smallest amount was 11.97 mg/kg on 35th. Nevertheless, the maximum amount of histamine was 55.04 mg/kg on the 0th day and about 23.14 mg/kg of histamine was little change from 14th to 35th days of storage. Dominant bacteria affect the change of biogenic amines. The study can help understand the interaction between microbial flora and biogenic amines in the salted mackerel of one‐night courtyard.

show abstract

“…The analysis methods and HPLC systems for biogenic amines determination were described in a previous research paper (Qiao et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

Interaction between bacterial diversity and biogenic amines production in a salted mackerel stored at soft frozen (−7℃–0℃) storage

Tao

Liu

et al. 2021

Food Science & Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

“…Acetonitrile was then added to make the final volume 5 ml. 1.0 ml mixture was filtered through a 0.45 μm filter for high-performance liquid chromatography (HPLC) analysis (Qiao et al, 2020). The concentrations of BAs were performed using a HPLC (Agilent, G1260, Agilent Technologies Co. Ltd., US) equipped with a C18 column (15 mm × 4.6 mm inner ID 5 μm) (Agilent Technologies Co. Ltd., US) and an ultraviolet detector.…”

Section: Bas Degradation and Generationmentioning

confidence: 99%

Evaluation of degradation capability of nitrite and biogenic amines of lactic acid bacteria isolated from pickles and potential in sausage fermentation

Chen

et al. 2021

Food Processing Preservation

Self Cite

View full text Add to dashboard Cite

To obtain nitrite‐degrading strains, 7 isolates of lactic acid bacteria (LABs) were selected and identified from pickles. Two Lactobacillus. plantarum strains presented considerably high biogenic amines (BAs) degradation abilities. Their degradation abilities of nitrite and BAs, growth rates and pH changes in Man‐Rogosa‐Sharpe (MRS) broth and the simulated medium (SM) for sausage fermentation were conducted, respectively. DPPH• scavenging activity was used for the evaluation of antioxidant activity. The results showed that they have both higher activity in degrading nitrite and BAs in MRS broth than SM. This probably was due to the lower population, and higher pH and salty in SM. The two L. plantarum isolates also showed excellent antioxidant capacity in the forms of culture of isolates and the extraction of broken cells. Therefore, the two L. plantarum isolates demonstrated the potential to be used as starters with degradation capability on some undesirable substances in fermentation of sausage. Novelty impact statement Two L. plantarum strains from Chinese pickles had high degradation abilities of nitrite and BAs. Degradation abilities of two L. plantarum strains were weaker in SM than MRS broth. Two L. plantarum strains had high antioxidant capability.

show abstract

“…Several attempts have been made to analyze BAs in various food products by using chromatographic instruments, including gas chromatography-mass spectrometry (GC-MS) [ 12 , 13 ] and high performance liquid chromatography [ 14 , 15 ]. Other analytical techniques, including capillary electrophoresis [ 16 ], liquid chromatography tandem-mass spectrometry (LC-MS/MS) [ 17 ], and ultra-high performance liquid chromatography [ 18 ] are also applied for the measurement of BAs.…”

Section: Introductionmentioning

confidence: 99%

The Measurement of Hazardous Biogenic Amines in Non-Alcoholic Beers: Efficient and Applicable Miniaturized Electro-Membrane Extraction Joined to Gas Chromatography-Mass Spectrometry

Kamankesh

Barzegar

Shariatifar

et al. 2023

Foods

View full text Add to dashboard Cite

The determination of biogenic amines (BAs) as serious food contaminants and chemical indicators of unwanted microbial contamination or deficient processing conditions in non-alcoholic beers is of great interest for the beverage industries. In the present investigation, the combination of hollow fiber-electro-membrane extraction (HF-EME) and dispersive liquid–liquid microextraction (DLLME) followed by gas chromatography-mass spectrometry (GC/MS) was applied for the analysis of histamine, putrescine, tyramine, cadaverine in non-alcoholic beers. EME is fundamentally based on the electrostatic attraction, diffusion and solvability of analytes in a selected acceptor phase. This membrane-based extraction technique promoted selectivity and the enrichment factor. The DLLME process reduced the volumes of organic solvents and make the coupling of HF-EME to the CG/MS conceivable. The leading variables, which have a great effect on extraction recovery, were optimized. The relative standard deviation was achieved between 4.9 and 7.0%. The recoveries were between 94% and 98%. The limit of detection and limit of quantification were found to be 0.92–0.98 ng mL−1 and 3.03–3.23 ng mL−1, respectively. The enrichment factor was calculated in the range 36–41. The achievements revealed that putrescine and tyramine, with concentrations of 3.87 and 2.33 µg g−1, were at the highest concentration in non-alcoholic beers. This offered method with great benefits could help beverage industries to monitor the concentration of BAs in beers and control them.

show abstract

Investigation of Biogenic Amines in Dried Bonito Flakes from Different Countries Using High-Performance Liquid Chromatography

Cited by 13 publications

References 33 publications

Interaction between bacterial diversity and biogenic amines production in a salted mackerel stored at soft frozen (−7℃–0℃) storage

Interaction between bacterial diversity and biogenic amines production in a salted mackerel stored at soft frozen (−7℃–0℃) storage

Evaluation of degradation capability of nitrite and biogenic amines of lactic acid bacteria isolated from pickles and potential in sausage fermentation

The Measurement of Hazardous Biogenic Amines in Non-Alcoholic Beers: Efficient and Applicable Miniaturized Electro-Membrane Extraction Joined to Gas Chromatography-Mass Spectrometry

Contact Info

Product

Resources

About