Investigation of Campylobacter in Reared Game Birds

Soncini, G.; Valnegri, L.; Vercellotti, L.; Colombo, Fabio; Valle, Denise; Franzoni, M.; Bersani, C.

doi:10.4315/0362-028x-69.12.3021

Cited by 9 publications

(7 citation statements)

References 9 publications

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“…This result could be due to the small sample size, however, it is confirmed by other surveys on Campylobacter, that show a low risk of infection for consumers. Indeed, C. jejuni was detected in 3.44% meat samples and 5.26% pigeon neck skin in Italy (Soncini et al, 2006), 13% intestinal contents in Japan (Ito et al, 1988), while no positive samples were observed in pigeon carcasses from retail markets in Egypt (Abd el Aziz et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Campylobacter jejuni, Campylobacter coli and enteric Helicobacter in domestic and free living birds in North-Western Italy

Robino¹,

Tomassone²,

Tramuta³

et al. 2010

Schweizer Archiv für Tierheilkunde

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In order to investigate the prevalence of some thermophilic Campylobacter (C. jejuni and C. coli) and enteric Helicobacter (H. pullorum and H. canadensis) in domestic and wild birds, a total of 278 bird caecal samples were analyzed over a 2 year period in North-Western Italy. Samples were collected from poultry raised in intensive farming at the slaughterhouse (n=102, group A) and in small scale rural farms (n=60, group B) as well as from wild birds (n=116, group C). PCR amplifications were carried out on DNA extracted from caecal samples. Molecular assays targeted the hipO gene for C. jejuni, the asp gene for C. coli and the 16S rRNA gene of H. pullorum/H. canadensis. To differentiate H. pullorum from H. canadensis, PCR products were subjected to an ApaLI digestion assay. Prevalence of thermophilic Campylobacter and enteric Helicobacter was significantly different among groups (p<0.0001). Campylobacter infections were detected in all three bird groups (78.4% group A, 18.3% group B and 38.8% group C, respectively), Helicobacter infections were only detected in poultry, with H. pullorum infecting 68.6% of group A and 21.7% of group B birds. H. canadensis was detected in Guinea fowls (group A) and for the first time in pheasants (group B). Mixed infections by enteric Campylobacter and Helicobacter were shown in 53.9% of group A and in 5.0 % of group B. Our results show that both microorganisms commonly infect poultry, especially intensive farming animals. Only hooded crows among the wild bird group (group C), proved to be highly sensitive to Campylobacter infection.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Campylobacter jejuni, Campylobacter coli and enteric Helicobacter in domestic and free living birds in North-Western Italy

Robino¹,

Tomassone²,

Tramuta³

et al. 2010

Schweizer Archiv für Tierheilkunde

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“…More than 600 million commercial pigeons are sold annually. Pigeon meat is consumed as a kind of nutritional food, and is gaining popularity among consumers in Europe mainly France and Italy 1 , 2 , United States 3 , Pakistan 4 and Japan 5 . Additionally, pigeon is also popular as racing and fancy bird in many areas of the world.…”

Section: Introductionmentioning

confidence: 99%

Changes in the diversity and composition of gut microbiota in pigeon squabs infected with Trichomonas gallinae

Feng

Zhang

Shao

et al. 2020

Sci Rep

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Pigeons, as the only altricial birds in poultry, are the primary Trichomonas gallinae (T. gallinae) host. To study the effects of T. gallinae infection on gut microbiota, we compared the microbiota diversity and composition in gastrointestinal (GI) tracts of pigeons at the age of 14 and 21 day with different degrees of T. gallinae infection. Thirty-six nestling pigeons were divided into three groups: the healthy group, low-grade and high-grade trichomonosis group. Then, the crop, small intestine and rectum contents were obtained for sequencing of the 16S rRNA gene V3–V4 hypervariable region. The results showed that the microbiota diversity was higher in crop than in small intestine and rectum, and the abundance of Lactobacillus genus was dominant in small intestine and rectum of healthy pigeons at 21 days. T. gallinae infection decreased the microbiota richness in crop at 14 days. The abundance of the Firmicutes phylum and Lactobacillus genus in small intestine of birds at 21 days were decreased by infection, however the abundances of Proteobacteria phylum and Enterococcus, Atopobium, Roseburia, Aeriscardovia and Peptostreptococcus genus increased. The above results indicated that crop had the highest microbiota diversity among GI tract of pigeons, and the gut microbiota diversity and composition of pigeon squabs were altered by T. gallinae infection.

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“…This pathogen is typically located in the feces and gastrointestinal tracts of warm-blooded animals [3], [4], [5], [6], which are considered reservoirs for human infection. Human campylobacteriosis is most often sporadic; although poorly prepared food has often been implicated, it is generally difficult to determine the source since C. jejuni easily loses cultivability in food specimens under aerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

Molecular Evidence for the Thriving of Campylobacter jejuni ST-4526 in Japan

et al. 2012

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Campylobacter jejuni is a leading cause of human gastroenteritis worldwide. This study aimed at a better understanding of the genetic diversity of this pathogen disseminated in Japan. We performed multilocus sequence typing (MLST) of Campylobacter jejuni isolated from different sources (100 human, 61 poultry, and 51 cattle isolates) in Japan between 2005 and 2006. This approach identified 62 sequence types (STs) and 19 clonal complexes (CCs), including 11 novel STs. These 62 STs were phylogenetically divided into 6 clusters, partially exhibiting host association. We identified a novel ST (ST-4526) that has never been reported in other countries; a phylogenetic analysis showed that ST-4526 and related STs showed distant lineage from the founder ST, ST-21 within CC-21. Comparative genome analysis was performed to investigate which properties could be responsible for the successful dissemination of ST-4526 in Japan. Results revealed that three representative ST-4526 isolates contained a putative island comprising the region from Cj0737 to Cj0744, which differed between the ST-4526 isolates and the reference strain NCTC11168 (ST-43/CC-21). Amino acid sequence alignment analyses showed that two of three ST-4526 isolates expressed 693aa- filamentous hemagglutination domain protein (FHA), while most of other C. jejuni strains whose genome were sequenced exhibited its truncation. Correspondingly, host cell binding of FHA-positive C. jejuni was greater than that of FHA-truncated strains, and exogenous administration of rFHA protein reduced cell adhesion of FHA-positive bacteria. Biochemical assays showed that this putative protein exhibited a dose-dependent binding affinity to heparan sulfate, indicating its adhesin activity. Moreover, ST-4526 showed increased antibiotic-resistance (nalidixic acid and fluoroquinolones) and a reduced ability for DNA uptake. Taken together, our data suggested that these combined features contributed to the clonal thriving of ST-4526 in Japan.

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Investigation of Campylobacter in Reared Game Birds

Cited by 9 publications

References 9 publications

Prevalence of Campylobacter jejuni, Campylobacter coli and enteric Helicobacter in domestic and free living birds in North-Western Italy

Prevalence of Campylobacter jejuni, Campylobacter coli and enteric Helicobacter in domestic and free living birds in North-Western Italy

Changes in the diversity and composition of gut microbiota in pigeon squabs infected with Trichomonas gallinae

Molecular Evidence for the Thriving of Campylobacter jejuni ST-4526 in Japan

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