Investigation of chemical modification on tosyl-activated polystyrene microsphere magnetic particle surface by infrared microscopy

Byun, Chang Kyu

doi:10.5806/ast.2016.29.5.225

Cited by 3 publications

(8 citation statements)

References 17 publications

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“…The modified particles were partitioned into the DEX-rich layer (Figure 5B), as in our previous studies [4]. The IR spectrum of the DEX-modified microspheres [5] proved that there were chemical changes after the reaction with amino-dextran (see Figure S1). We tested the microspheres without the BSA reaction steps, which are normally used to replace unreacted tosyl residues on the microbeads.…”

Section: Partitioning Of Amino-dextran (Adex)-modified Microbeads In supporting

confidence: 76%

“…Figure 5D shows that the BSA-modified Dynabeads ® were partitioned into the DEX-rich layer. In addition, the IR spectrum for the modified particles revealed the attachment of BSA on the modified microparticles [5] (also see Figure S1). …”

Section: Partitioning Of Bovine Serum Albumin (Bsa)-modified Microbeamentioning

confidence: 96%

“…In addition, it has a similar average MW to that of the previously used aDEX (MW 10,000). The IR spectra of the PEG-modified Dynabeads ® , established that the methoxy-PEG-amine (mPEG) modification was successful according to the IR peak of the amino bond after the reaction [5] (see Figure S1). The PEG-modified Dynabead ® was partitioned into the PEG-rich upper layer ( Figure 5C), similar to the unmodified tosyl-activated Dynabead ® ( Figure 5A).…”

Section: Partitioning Of Methoxy-polyethylene Glycol Amine (Mpeg)-modmentioning

confidence: 98%

“…IR spectral investigation of the tosyl-activated Dynabeads ® [5] showed that the tosyl functional group exists on the Dynabead surface, and both polystyrene and the tosyl group on the Dynabead M-280 surface are hydrophobic, which is the driving force of partition to upper PEG-rich layer.…”

Section: Partitioning Of Unmodified Ps Microbeads In Peg-dex Atpsmentioning

confidence: 99%

“…Our motive and the challenge of studying partition was informed by these points of view. Recently, a prerequisite infrared (IR) study was attempted and published as a first step in solving the above questions [5]. Here, we aim to investigate the partition effect of surface modifying polystyrene (PS)-coated magnetic microparticles in a PEG-DEX aqueous two-phase systems (ATPS).…”

Section: Introductionmentioning

confidence: 99%

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Modulating the Partitioning of Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Biphasic System by Surface Modification

Byun

Kim

2018

Coatings

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Aqueous two-phase systems (ATPSs) or aqueous biphasic systems are useful for biological separation/preparation and cell micropatterning. Specifically, aqueous two-phase systems (ATPSs) are not harmful to cells or biomaterials; therefore, they have been used to partition and isolate these materials from others. In this study, we suggest chemically modifying the surface of target materials (micro/nanoparticles, for example) with polymers, such as polyethylene glycol and dextran, which are the same polymer solutes as those in the ATPS. As a simple model, we chemically coated polyethylene glycol or dextran to the surface of polystyrene magnetic particles and observed selective partitioning of the surface modified particles to the phase in which the same polymer solutes are dominant. This approach follows the principle "like dissolves like" and can be expanded to other aqueous biphasic or multiphasic systems while consuming fewer chemicals than the conventional modulation of hydrophobicities of solute polymers to control partitioning in aqueous biphasic or multiphasic systems.

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Section: Partitioning Of Amino-dextran (Adex)-modified Microbeads In supporting

confidence: 76%

Section: Partitioning Of Bovine Serum Albumin (Bsa)-modified Microbeamentioning

confidence: 96%

Section: Partitioning Of Methoxy-polyethylene Glycol Amine (Mpeg)-modmentioning

confidence: 98%

Section: Partitioning Of Unmodified Ps Microbeads In Peg-dex Atpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modulating the Partitioning of Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Biphasic System by Surface Modification

Byun

Kim

2018

Coatings

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A plasma ion bombardment process enabling reagent-free covalent binding of multiple functional molecules onto magnetic particles

Tran

Craggs²,

Smith³

et al. 2019

Materials Science and Engineering: C

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A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection

Razzino,

Sgobbi,

Cancino-Bernardi

et al. 2024

Front. Sens.

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Alzheimer’s disease is the most prevalent form of dementia and is primarily characterized by the accumulation of β-amyloid and phosphorylated tau proteins in the brain, along with the degeneration of nerve cells, which leads to impairment of various cognitive functions. A significant biomarker of Alzheimer’s disease is the decreased level of soluble β-amyloid peptide (1–42) (Aβ1-42) in cerebrospinal fluid (CSF), as pathology progresses when CSF-Aβ1-42 levels drop below 192 pg mL−1. In this study, we developed an amperometric immunosensor based on magnetic beads as the platform for constructing the immunosensor. Monoclonal antibodies are immobilized on the MBs, enabling selective detection of Aβ1-42. The detection antibody is conjugated with the enzyme horseradish peroxidase, which, in the presence of H2O2 and hydroquinone, catalyzes the decomposition of H2O2 and the oxidation of hydroquinone to p-quinone, generating an electric current measured at a potential of −200 mV (vs. the Ag pseudo-reference electrode) using screen-printed carbon electrodes. The amperometric sandwich-type immunosensor demonstrates a linear response in the concentration range of 10 to 10,000 pg mL−1, with a detection limit of 7.4 pg mL−1, exhibiting excellent selectivity against the assessed interferents. These findings suggest the potential application of this immunosensor in the early diagnosis of Alzheimer’s disease, offering a sensitive and specific tool for clinical analysis. Despite its high performance, further studies are required to validate its robustness and applicability in complex clinical samples.

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Investigation of chemical modification on tosyl-activated polystyrene microsphere magnetic particle surface by infrared microscopy

Cited by 3 publications

References 17 publications

Modulating the Partitioning of Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Biphasic System by Surface Modification

Modulating the Partitioning of Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Biphasic System by Surface Modification

A plasma ion bombardment process enabling reagent-free covalent binding of multiple functional molecules onto magnetic particles

A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection

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