Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

Tresha, Arunima Oarin; Arif, Mohammad; Islam, SK Shaheenur; Haque, Anowarul; Rahman, Md. Tanvir; Kabir, S. M. Lutful

doi:10.14202/vetworld.2021.2809-2816

Cited by 6 publications

(7 citation statements)

References 47 publications

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“…These study findings could not compare explicitly in country context due to the lack of substantial reference data on the prevalence rate of NE layer chicken in Bangladesh. However, a recent study confirmed a flock level prevalence of C. perfringens as 10.3% in broiler chicken (Tresha et al, 2021). Additionally, a few past studies established the incidence rate of C. perfringens that varies from 0.4 to 1% based on clinical findings and post mortem lesions (Hossain et al, 2012;Islam et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

Arif

Sultana

Islam

et al. 2022

Asian Australas. J. Biosci. Biotechnol.

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Necrotic enteritis (NE) is one of the important enteric diseases in poultry caused by Clostridium perfringens which leads to considerable economic losses for poultry raisers. The present research was carried out to estimate prevalence, risk factors and to confirm molecular pattern, antimicrobial susceptibility status of Clostridium perfringens in layer flocks in Mymensingh and Gazipur districts of Bangladesh, where a total of 287 samples were collected that comprised of water (30), workers’ hand washing (30), and feed (30) as environmental pooled samples; cloacal swab (150) and post mortem sample (intestine) (47) as individual samples, and evaluated by basic (culture, biochemical test), and finally toxinotyping multiplex PCR assays. The flock-level data on potential risk factors were collected through semi-structured interviews with the farm owners and evaluated via univariable and multivariable logistic regression analyses where a p-value of <0.05 was considered as statistically significant. Overall flock-level prevalence of Clostridium perfringens was confirmed to be 6.3% (95% confidence interval [CI]: 3.8-9.7%). The occurrence of C. perfringens was found to be higher in the intestine samples (12.8%, 95% CI: 4.8- 25.7%) followed by feed (6.7%, 95% CI: 0.8-22.1%), cloacal swabs (6.0%, 95% CI: 2.8-11.1%) and workers’ hand washing (3.3%, 95% CI: 0.1- 12.2%). History of immune-suppressive disease (like New castle disease/egg drop syndrome/infectious bursal disease) [Odds Ratio (OR) = 22.16, 95% CI: 1.40-349.36, P = 0.027] and flock size more than 3000 birds (OR = 11.16, 95% CI: 1.26-98.25, P = 0.029) were found to be associated with C. perfringens infection status in the layer flocks. In this study, C. perfringens toxinotype A is circulating in layer flocks with 72.2% of isolates were established as multidrug-resistant as they demonstrated resistance between 3 to 6 antimicrobial agents. Evidence-based control measures to be taken namely use of probiotics, prebiotic, organic acids, and plant extracts as natural alternatives including control of immune-suppressive diseases are needed to minimize the NE infection and antimicrobial resistance in low biosecurity layer flocks. Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 36-49

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Section: Discussionmentioning

confidence: 98%

Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

Arif

Sultana

Islam

et al. 2022

Asian Australas. J. Biosci. Biotechnol.

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“…Total genomic DNA was extracted from pure colonies of the bacteria using the Total DNA Extraction Kit as described by Tresha et al (2021). The size (236 bp of the beta toxin of C. perfringens) of the target gene (CPB gene) was amplified and the Polymerase Chain Reaction (PCR) was carried out in a Thermal Cycler (2720 Thermal Cycler; Applied Biosystem).…”

Section: Molecular Testmentioning

confidence: 99%

Toxin-producing Clostridium perfringens in cooked cereal food in restaurants in Bangladesh

MA,

et al. 2024

Bangl. vet.

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Clostridium perfringens causes food poisoning in humans worldwide and C. perfringens beta toxin is associated with improperly heated or reheated cooked food. A study was undertaken to determine the prevalence of beta toxin-producing C. perfringens in cooked Plain rice, Pulao, and Biryani in different types of restaurants (general, well furnished) of four districts [Dhaka (north and south city corporation area), Cumilla, Narayangonj, Gazipur)] of Bangladesh. A total of 200 food samples were examined for the presence of C. perfringens and its beta toxin. The positive samples were further tested for the CPB gene (236 bp) of beta toxin-producing C. perfringens using PCR assay. Three samples had C. perfringens (1.5%), in food samples of the restaurants in Dhaka South City Corporation (DSCC). Beta toxin-producing C. perfringens was in one sample (0.5%) in a sample of pulao in the same area during the winter. It is suggested that the prevalence of beta toxin-producing C. perfringens was low but, further studies are required in other cities in Bangladesh. Bangl. vet. 2023. Vol. 40, No. 1-2, 8–15

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“…The improper antimicrobial drug used for humans is diffusive in developing countries and is a significant contributor to growing the public health threat of AMR-resistant bacteria (Roess et al, 2013). In recent years, the indiscriminate use of antibiotics in animals and poultry is thought to be an important factor to develop of AMR Al-Salauddin et al, 2015;Sohidullah et al, 2016;Rahman et al, 2016;Talukder and Ahmed, 2016;Roy et al, 2017;Jahan et al, 2017;Kabir et al, 2018a;Kabir et al, 2018b;Kamal et al, 2018;Alam et al, 2019;Rumi et al, 2019;Saif et al, 2019;Masud et al, 2020;Alam et al, 2020;Sarker et al, 2020;Mridha et al, 2020;Matubber et al, 2021;Uddin et al, 2021;Tresha et al, 2021;Eashmen et al, 2021;Haque et al, 2021;Hosain et al, 2021;Arif et al, 2022;Hoque et al, 2022). In Bangladesh, the most common reason for choosing an antimicrobial is personal experience and perception (68%), rather than the cultural sensitivity test which may be due to a lack of vet diagnostic facilities and the unwillingness of the veterinary personnel (Pokharel et al, 2020).…”

Section: Development Of Antimicrobial Resistance (Amr)mentioning

confidence: 99%

Recent trends and scenario of antibiotic use in veterinary practices for livestock production in Bangladesh: a review

Hosain

Islam

Kamal

et al. 2022

Asian Australas. J. Biosci. Biotechnol.

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Antibiotics are used for the treatment and control of diseases as well as a growth promoter in livestock production in Bangladesh. The frequent use of these antibiotics in veterinary practices may lead to residue and creates some potential problems not only in livestock but also in public health issues. The presence of residues of antibiotics in animal-derived foodstuffs may induce carcinogenic and mutagenic effects and leads to the condition of an allergic reaction, and the development of antibiotic resistance in human gut bacteria. Although misuse of antibiotics in human medicine is the principal cause of the problems, some antibiotic-resistant bacteria originating in animals may also be the contributory factors to the resistance. However, some of the antibiotic resistance problems can be attributed to the transfer of resistant bacteria from animals to humans and resistance genes from animal pathogens and commensal bacteria to human pathogens. Even though the antibiotics and their residues in feed and food products of animal origin cause serious public health problems but little is known by society due to the lack of proper information. So, this is important to review the uses of antibiotics in food animals and insight their public health significance in Bangladesh. However, the safety levels of animal feeds and food of animal origin must be strictly observed and the antibiotics should be used in accordance with the labeled directions public awareness should be developed about the indiscriminate use of antibiotics in animals and their hazardous residual impacts on the human body. Therefore, the present review focuses on the aspects of antibiotic use in the livestock production of Bangladesh. Asian Australas. J. Biosci. Biotechnol. 2022, 7 (3), 90-106

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Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

Cited by 6 publications

References 47 publications

Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

Toxin-producing Clostridium perfringens in cooked cereal food in restaurants in Bangladesh

Recent trends and scenario of antibiotic use in veterinary practices for livestock production in Bangladesh: a review

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