Investigation of flexible electrodes modified by TiN, Pt black and IrO x

Li, Xiaoqian; Tang, Rongyu; Gui, Qiang; Guo, Kai; Wang, Yu; Chen, Hongda

doi:10.1007/s11431-011-4436-7

Cited by 14 publications

(13 citation statements)

References 20 publications

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“…That is in line with our result for sputtered TiN, 2.0 mC/cm 2 . On the contrary, Gerwig et al (2012) and Li et al (2011) have reported both only 0.45 mC/cm 2 . So, if Janders' record is ignored, IBAD TiN seems to perform well against sputtered TiN with its CTC of 3.3 mC/cm 2 also in this aspect.…”

Section: Discussionmentioning

confidence: 92%

Ion Beam Assisted E-Beam Deposited TiN Microelectrodes—Applied to Neuronal Cell Culture Medium Evaluation

et al. 2018

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Microelectrode material and cell culture medium have significant roles in the signal-to-noise ratio and cell well-being in in vitro electrophysiological studies. Here, we report an ion beam assisted e-beam deposition (IBAD) based process as an alternative titanium nitride (TiN) deposition method for sputtering in the fabrication of state-of-the-art TiN microelectrode arrays (MEAs). The effects of evaporation and nitrogen flow rates were evaluated while developing the IBAD TiN deposition process. Moreover, the produced IBAD TiN microelectrodes were characterized by impedance, charge transfer capacity (CTC) and noise measurements for electrical properties, AFM and SEM for topological imaging, and EDS for material composition. The impedance (at 1 kHz) of brand new 30 μm IBAD TiN microelectrodes was found to be double but still below 100 kΩ compared with commercial reference MEAs with sputtered TiN microelectrodes of the same size. On the contrary, the noise level of IBAD TiN MEAs was lower compared with that of commercial sputtered TiN MEAs in equal conditions. In CTC IBAD TiN electrodes (3.3 mC/cm2) also outperformed the sputtered counterparts (2.0 mC/cm2). To verify the suitability of IBAD TiN microelectrodes for cell measurements, human pluripotent stem cell (hPSC)-derived neuronal networks were cultured on IBAD TiN MEAs and commercial sputtered TiN MEAs in two different media: neural differentiation medium (NDM) and BrainPhys (BPH). The effect of cell culture media to hPSC derived neuronal networks was evaluated to gain more stable and more active networks. Higher spontaneous activity levels were measured from the neuronal networks cultured in BPH compared with those in NDM in both MEA types. However, BPH caused more problems in cell survival in long-term cultures by inducing neuronal network retraction and clump formation after 1–2 weeks. In addition, BPH was found to corrode the Si3N4 insulator layer more than NDM medium. The developed IBAD TiN process gives MEA manufacturers more choices to choose which method to use to deposit TiN electrodes and the medium evaluation results remind that not only electrode material but also insulator layer and cell culturing medium have crucial role in successful long term MEA measurements.

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Section: Discussionmentioning

confidence: 92%

Ion Beam Assisted E-Beam Deposited TiN Microelectrodes—Applied to Neuronal Cell Culture Medium Evaluation

et al. 2018

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“…MEA are commonly coated with high porosity materials to improve performances.. The commonly used coating materials include platinum black, titanium nitride (TiN) [1], iridium oxide (IrOx) [2,3], carbon nanotubes (CNT) [4,5] and conductive polymers [6,7]. The shared purposes of these coatings were to increase the effective surface areas (ESA) and therefore to reduce the interfacial impedance, thermal noise and raise the sensitivity of the microelectrodes.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of strongly adherent platinum black coatings on microelectrodes array

Tang

Chen

Zhao

et al. 2013

Sci. China Inf. Sci.

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Microscope in situ FTIRS studies of CO adsorption on an array of platinum micro-electrodes Chinese Science Bulletin 46, 1612 (2001); Fabrication of nanometer-sized gold flower microelectrodes for electrochemical biosensing applications SCIENTIA SINICA Chimica 45, 1214 (2015); Fabrication of sub-micro size PZT array and their ferroelectric properties Science in China Series E-Technological Sciences 49, 693 (2006);. RESEARCH PAPER. SCIENCE CHINA Information Sciences

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“…Surprisingly, despite this advantage, ITO is largely under-used compared to gold. Moreover, the electrode coatings explored to enhance the performance of electrodes for neural applications [6] are mostly considered as a strategy to modify the sensitivity of ECIS electrodes [7]. In this paper, a comparison between IDEs of ITO and ITO coated with IrOx is presented and their sensitivities are studied in response to the proliferation of the human breast cancer cell line, MCF-7.…”

Section: Introductionmentioning

confidence: 99%

Comparison of ITO and IrOx-Modified ITO Interdigitated Electrodes for Electrical Cell-Substrate Impedance Sensing (ECIS) Applications

Martínez

Montalibet

McAdams

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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This study compares the sensitivity of interdigitated electrodes (IDEs) used in Electrical CellSubstrate Impedance Sensing (ECIS). IDEs made of indium tin oxide (ITO) and ITO coated with iridium oxide (IrOx) were used for ECIS measurements with the human breast cancer cell line, MCF-7. The results indicate that IrOx-modified ITO electrodes provide both biocompatibility and higher cell sensitivity compared to ITO electrodes. We also found that the quantity of IrOx required to generate such a sensitivity improvement is sufficiently low to not interfere with the visualization of the cells under study. IrOx-modified ITO electrodes are therefore promising sensors for ECIS applications.

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Investigation of flexible electrodes modified by TiN, Pt black and IrO x

Cited by 14 publications

References 20 publications

Ion Beam Assisted E-Beam Deposited TiN Microelectrodes—Applied to Neuronal Cell Culture Medium Evaluation

Ion Beam Assisted E-Beam Deposited TiN Microelectrodes—Applied to Neuronal Cell Culture Medium Evaluation

Fabrication of strongly adherent platinum black coatings on microelectrodes array

Comparison of ITO and IrOx-Modified ITO Interdigitated Electrodes for Electrical Cell-Substrate Impedance Sensing (ECIS) Applications

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