Investigation of Gene Polymorphisms of Vaspin, Visfatin and Chemerin in Diabetic Obese and Non-Diabetic Obese Patients.

Abstract

Diabetes mellitus and obesity are very similar in terms of pathogenesis and pathophysiology. Most obese patients have adipose tissue dysfunction caused by genetic and environmental factors. Adipose tissue is the source of adipokines secreted from adipocytes. Vaspin (visceral adşpose tissue-derived serine protease inhibitor), Visfatin (pre-B cell enhancing factor) and Chemerin are adipokines discovered in recent years. The study consists of 43 non-diabetic obese and 51 diabetic obese individuals. The PCR-RFLP method and agarose gel electrophoresis techniques were used to detect gene polymorphisms of Chemerin rs17173608, Vaspin rs2236242 and Visfatin rs2110385 from DNA samples. In our study, when diabetic obese and non-diabetic obese patient groups were examined in terms of Vaspin gene polymorphism, statistically significant results were obtained (22% → 7%, p=0.048, respectively). The distribution of Chemerin or Visfatin gene variants were not different in study groups (p>0.05). Our results indicate that Chemerin rs17173608 and Visfatin rs2110385 gene polymorphisms were not risk factors for development of diabetes in obese individuals, however, Vaspin rs2236242 gene polymorphism may be a contributory risk of development of diabetes in obese individuals.

Mitofaji ve Obezite

“…Increased accumulation of fatty acid causes adipocyte hypertrophy and impaired bloodstream by triggering the production of proinflammatory cytokines, particularly via the nuclear factor kappa B (NF-κB) signaling pathway. As well as enlargement of the volume of infiltrated macrophages causes synthesis of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), adiponectin, visfatin, interleukin-1β (IL-1β), and interleukin-6 (IL-6), in adipocytes that are secreted from infiltrated macrophages that lead to the process described as low-grade chronic inflammation (7,17,18). Low-level chronic inflammation in the long-term may turn into chronic systemic inflammation and lead to the occurrence and advancement of metabolism-related disorders, such as IR, hyperlipidemias, hypercholesterolemia, T2DM, cardiovascular diseases, and atherosclerosis (10)(11)(12).…”

Genetic Investigation of the Trail Mechanism in Diabetic and Non-diabetic Obese Patients

“…Diabetes is a metabolic disease that is characterized by elevated blood glucose levels because of impaired insulin secretion and/or activity in cells in the Langerhans Islets of the pancreas [5]. Studies have shown that the relationship between diabetes and obesity is the main cause of insulin resistance which results from body fat distribution, TNF-α, free fatty acids, and gene defects of the β3-adrenergic receptor in the body [6,7].…”