Investigation of Heavy Metals Biosorption on Pseudomonas aeruginosa Strain MCCB 102 Isolated from the Persian Gulf

Zolgharnein, Hossein; Karami, Kazem; Assadi, Mahnaz Mazaheri; Sohrab, Ali Dadolahi

doi:10.3923/ajbkr.2010.99.109

Cited by 26 publications

(8 citation statements)

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“…Study of the morphological characteristics of the arsenic treated cells showed the appearance of long bacilli under SEM depicting the mode of response of bacteria to arsenic stress. The result is consistent with the studies of Zolgharnein et al [38] who has reported an increase in cell size of Pseudomonas aeruginosa coupled with Cd and Pb accumulation. Cell uptake metals by increasing the area of contact which is a positive response to well adapt to the toxic environment [6,24].…”

Section: Discussionsupporting

confidence: 93%

Arsenic resistance and accumulation by two bacteria isolated from a natural arsenic contaminated site

Pandey

Bhatt

2015

J. Basic Microbiol.

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Forty-three indigenous arsenic resistant bacteria were isolated from arsenic rich soil of Rajnandgaon district in the state of Chhattisgarh, India by enrichment culture technique. Among the isolates, two of the bacteria (As-9 and As-14) exhibited high resistance to As(V) [MIC ≥ 700 mM] and As(III) [MIC ≥ 10 mM] and were selected for further studies. Both these bacteria grew well in the presence of arsenic [20 mM As(V) and 5 mM As(III)], but the isolate As-14 strictly required arsenic for its survival and growth and was characterized as a novel arsenic dependent bacterium. The isolates contributed to 99% removal of arsenic from the growth medium which was efficiently accumulated in the cell. Quantitative estimation of arsenic through Atomic Absorption Spectrophotometer revealed that there was >60% accumulation of both As(V) and As(III) by the two isolates. Scanning Electron Microscopic analysis showed a fourfold increase in bacterial cell volume when grown in the presence of arsenic and the results of Transmission Electron Microscopy and energy-dispersive X-ray spectroscopy proved that such an alteration was due to arsenic accumulation. Such arsenic resistant bacteria with efficient accumulating property could be effectively applied in the treatment of arsenic contaminated water.

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Section: Discussionsupporting

confidence: 93%

Arsenic resistance and accumulation by two bacteria isolated from a natural arsenic contaminated site

Pandey

Bhatt

2015

J. Basic Microbiol.

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“…In this study, Bacillus Licheniformis (71.3 %, 70.1 %, 66.5 %), Pseudomonas aeruginosa (69.3 %, 68.6 %, 74.1 %), Aspergillus flavus (68.4 %, 58.6 %, 61.8 %) and Aspergillus niger (72.0 %, 69.7 %, 78.3 %) were found to have a very high bio-accumulation capacity for Cu, Pb and Zn. The high accumulation ability of these organisms may be due to the presence of teichoic and teichuronic acids, which were the principal sites of metal binding in the microbial cell walls [30]. This ability of Bacillus licheniformis to have very high bioaccumulation ability in this study was similar to the result Sulaimon et al [6] who also reported Bacillus licheniformis to have bioaccumulation percentage of 90.4 % for both Cu and As.…”

Section: Discussionsupporting

confidence: 88%

“…The highest adsorption in this study was observed for Cu. Zolgharnein [30] reported significant uptake of heavy metals ions by Pseudomonas aeruginosa strain MCCB 102 isolated from the Persian Gulf; however, in the current study, the highest adsorption was observed for Cu, Zn, and Pb.…”

Section: Discussioncontrasting

confidence: 76%

Bioaccumulation of Heavy Metals using Selected Organisms Isolated from Electronic Waste Dumpsite of two South-Western States in Nigeria

et al. 2017

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Heavy metals from electronic wastes can accumulate to alarming concentrations in soils, causing significant detrimental impacts on human life and the environment. Bioaccumulation of heavy metals by bacteria and fungi has been a major focus of most bioremediation studies owing to the excellent metal-binding properties. The current study was conducted to isolate the most promising Zn, Cu and Pb tolerant microorganisms from contaminated soils, and to assess their metal accumulating abilities. Bacillus licheniformis, B. polymyxa, Pseudomonas aeruginosa, Micrococcus roseus, Aspergillus niger and A. flavus were selected for the bioaccumulation study, based on their known tolerance to heavy metals. Bacillus licheniformis was most efficient in the removal of Cu (71.3 %) and Pb (70.1 %). Pb accumulation for Aspergillus flavus was 65.76 %. Zn accumulation for Pseudomonas aeruginosa and Aspergillus niger were 74.1 % and 78.3 %, respectively. This study concluded that all these microorganisms have potential for bioremediating soil environments contaminated with heavy metals.

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“…Moreover, the data of AAS was substantiated by SEM. It had been reported earlier that SEM analysis of Pseudomonas aeruginosa strain MCCB 102 showed an increase in cell size due to Cd and lead accumulation in the cell wall and along the external cell surfaces (Zolgharnein et al, 2010). The effect of metal on cell morphology was also demonstrated by transmission electron microscopy analysis of P. putida strain 62BN which showed an increase in size of the cells grown in the presence of Cd and also showed intracellular and periplasmic accumulation of metal in the cells (Rani et al, 2009).…”

Section: Atomic Absorption Spectroscopy and Scanning Electron Microscopymentioning

confidence: 60%

Mechanism of arsenic resistance prevalent in Bacillus species isolated from soil and ground water sources of India

Saluja¹,

Gupta²,

Goel³

2011

Ekologija

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Arsenic is a naturally occurring element and its contamination of soil and groundwater is a major threat to the environment and human health. The ubiquity of arsenic in the environment has led to the evolution of the arsenic defence mechanism in certain microbes. The present study highlights identical mechanisms of arsenic resistance in two bacterial strains, namely AG24 and AGM13, which were taken from two different ecosources and states of India. The phylogenetic analysis of the strains using 16S rDNA sequence revealed both strains to be under the genus 'Bacillus'. Furthermore, arsC gene was amplified from the strains, cloned and sequenced. The homology of the sequences of the two strains, when compared with available database using BLASTn search, showed that the 300bp amplicons obtained in both strains possess a partial arsC gene sequence which codes for arsenate reductase, an enzyme involved in the reduction of arsenate to arsenite, which is then effluxed out of the cell. Nevertheless, atomic absorption spectroscopy and scanning electron microscopy analysis of both strains also strengthen the presence of the efflux mechanism. Thus, the presence of a similar mechanism of resistance in both strains suggests the possible role of lateral gene transfer from soil to water system and vice versa which is an alarming situation for global concern.

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Investigation of Heavy Metals Biosorption on Pseudomonas aeruginosa Strain MCCB 102 Isolated from the Persian Gulf

Cited by 26 publications

References 0 publications

Arsenic resistance and accumulation by two bacteria isolated from a natural arsenic contaminated site

Arsenic resistance and accumulation by two bacteria isolated from a natural arsenic contaminated site

Bioaccumulation of Heavy Metals using Selected Organisms Isolated from Electronic Waste Dumpsite of two South-Western States in Nigeria

Mechanism of arsenic resistance prevalent in Bacillus species isolated from soil and ground water sources of India

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