Investigation of interaction between the drug and cell membrane by capillary electrophoresis

Abstract

By introducing cell membrane into electrophoretic buffer as pseudo-stationary phase, a novel capillary electrophoresis method was established to explore the interaction between drugs and cell membrane, where the interaction between citalopram and rabbit red blood cell membrane was used as an example. A series of concentrations of cell membrane were suspended into the running buffer by peak-shift method. The binding constant of citalopram to rabbit red blood cell membrane of 0.977 g -1 ·L was obtained after treatment of Scatchard plot. This method could provide not only a new way for the investigation on the interactions between drugs and cell membrane, but also a new approach for high throughput screening of the drug membrane permeability, biological activity, and evaluating drugs in vivo.

Thermodynamics of Antimicrobial Peptide JCpep8 Binding to Living Staphylococcus aureus as a Pseudo-stationary Phase in Capillary Electrochromatography and Consequences for Antimicrobial Activity

“…Consequently, the binding constant measured in our paper was the total value of the binding constant, which contained all of the binding capacities of the sum of the binding sites. Similar results were reported by Berthod et al 26 and Xia et al 32 The reproducibility of the results was confirmed by repeating the whole experiment at different temperatures, and all of the repeating measurements were performed in the same day with the same batch of bacteria.…”

Characterizations of interaction between antimicrobial peptide (JCpep7) and living staphylococcus aureus used as pseudostationary phase in capillary electrochromatography

“…The binding constant of JCpep7 to LSACs determined from this linear plot was 3.85 Â 10 12 M À1 at 298 K, 5.04 Â 10 12 M À1 at 303 K and 6.73 Â 10 12 M À1 at 309 K, respectively. Similar results were reported by Berthod et al 14 (the binding constant of vancomycin to B.infantis cells, 1.0 AE 0.1 Â 10 13 M À1 ) and Xia et al 35 (the binding constant of citalopram to rabbit red blood cell membrane, 5.4 Â 10 4 M À1 ). The results show that the interaction between JCpep7 and LSACs was significant.…”