Investigation of liquid biopsy analytes in peripheral blood of individuals after SARS-CoV-2 infection

Qi, Elizabeth; Courcoubetis, George; Liljegren, Emmett; Herrera, Ergueen; Nguyen, Nathalie; Nadri, Maimoona; Ghandehari, Sara; Davoodi, Sayed Hossein; Reckamp, Karen L.; Merin, Noah; Merchant, Akil; Mason, Jeremy; Figueiredo, Jane C.; Shishido, Stephanie N.; Kühn, Peter

doi:10.1016/j.ebiom.2023.104519

Cited by 6 publications

(7 citation statements)

References 34 publications

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“…We did not calculate minimal sample size to achieve a given statistical power because this is an exploratory study ( 28 , 29 ) and we are unaware of other studies with a similar design and purpose. Recent studies of the protective effects of precooling and curcumin on organ injury among patients with EHS included only 8–12 participants ( 30 , 31 ).…”

Section: Methods and Analysismentioning

confidence: 99%

Comparison of immune cell profiles associated with heatstroke, sepsis, or cardiopulmonary bypass: Study protocol for an exploratory, case-control study trial

Yang

Wang

et al. 2023

Front. Med.

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IntroductionHeatstroke is a life-threatening illness involving extreme hyperthermia and multi-organ failure, and it is associated with high mortality. The immune profiles of heatstroke have not been fully elucidated, and diagnostic and prognostic biomarkers of heatstroke are lacking. This study will analyze immune profiles in heatstroke patients as they differ from profiles in patients with sepsis or aseptic inflammation patients in order to identify diagnostic and prognostic biomarkers.MethodsThis exploratory, case–control study will recruit patients with heatstroke, patients with sepsis, patients undergoing cardiopulmonary bypass as well as healthy controls at West China Hospital of Sichuan University from 1 January 2023 to 31 October 2023. The four cohorts will be profiled at one time point in terms of lymphocytes, monocytes, natural killer cells, and granulocytes using flow cytometry, and cell populations will be visualized in two dimensions using t-SNE and UMAP, then clustered using PhenoGraph and FlowSOM. Gene expression in the specific immune cell populations will also be compared across the four cohorts, as will levels of plasma cytokines using enzyme-linked immunosorbent assays. Outcomes in the cohorts will be monitored during 30-day follow-up.DiscussionThis trial is, to our knowledge, the first attempt to improve the diagnosis of heatstroke and prediction of prognosis based on immune cell profiles. The study is also likely to generate new insights into immune responses during heatstroke, which may help clarify the disease process and lay the foundation for immunotherapies.

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Section: Methods and Analysismentioning

confidence: 99%

Comparison of immune cell profiles associated with heatstroke, sepsis, or cardiopulmonary bypass: Study protocol for an exploratory, case-control study trial

Yang

Wang

et al. 2023

Front. Med.

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“…Researchers applied the liquid biopsy assay on peripheral blood specimens collected from patients who had a prior SARS‐CoV‐2 infection as well as non‐COVID‐19 peripheral blood samples drawn prepandemic. In samples suspected of long COVID, increased platelet structures and endothelial cells count were detected 195 …”

Section: Diagnosis Of Viral Persistence In Long Covid Patientsmentioning

confidence: 98%

“…In samples suspected of long COVID, increased platelet structures and endothelial cells count were detected. 195 In a case report, Goh et al 35 could detect residual SARS-CoV-2 antigens and RNA in tissues of the skin, appendix, and breast of two patients with long COVID, 23 and 61 weeks after symptom onset. 35 The skin and appendix tissues of the first patient (a 44-year-old woman who has peritonitis and appendiceal lymphoid hyperplasia) were collected 61 weeks after initial symptom onset.…”

Section: Innatementioning

confidence: 99%

“…184 The possibility that patients carry a liquid biopsy signature in their peripheral blood after SARS-CoV-2 infection was investigated. 195 Researchers applied the liquid biopsy assay on peripheral blood specimens collected from patients who had a prior SARS-CoV-2 infection as well as non-COVID-19 peripheral blood samples drawn prepandemic. In samples suspected of long COVID, increased platelet structures and endothelial cells count were detected.…”

Section: Innatementioning

confidence: 99%

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Intrinsic factors behind long COVID: III. Persistence of SARS‐CoV‐2 and its components

El‐Baky,

Amara,

Uversky

et al. 2023

J of Cellular Biochemistry

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Considerable research has been done in investigating SARS‐CoV‐2 infection, its characteristics, and host immune response. However, debate is still ongoing over the emergence of post‐acute sequelae of SARS‐CoV‐2 infection (PASC). A multitude of long‐lasting symptoms have been reported several weeks after the primary acute SARS‐CoV‐2 infection that resemble several other viral infections. Thousands of research articles have described various post‐COVID‐19 conditions. Yet, the evidence around these ongoing health problems, the reasons behind them, and their molecular underpinnings are scarce. These persistent symptoms are also known as long COVID‐19. The persistence of SARS‐CoV‐2 and/or its components in host tissues can lead to long COVID. For example, the presence of viral nucleocapsid protein and RNA was detected in the skin, appendix, and breast tissues of some long COVID patients. The persistence of viral RNA was reported in multiple anatomic sites, including non‐respiratory tissues such as the adrenal gland, ocular tissue, small intestine, lymph nodes, myocardium, and sciatic nerve. Distinctive viral spike sequence variants were also found in non‐respiratory tissues. Interestingly, prolonged detection of viral subgenomic RNA was observed across all tissues, sometimes in multiple tissues of the same patient, which likely reflects recent but defective viral replication. Moreover, the persistence of SARS‐CoV‐2 RNA was noticed throughout the brain at autopsy, as late as 230 days following symptom onset among unvaccinated patients who died of severe infection. Here, we review the persistence of SARS‐CoV‐2 and its components as an intrinsic factor behind long COVID. We also highlight the immunological consequences of this viral persistence.

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“…41 In infectious diseases, liquid biopsy techniques could hasten pathogen detection and genomic tracing, leading to quicker therapeutic responses. 64 The potential for transformative discoveries in rheumatology, cardiology, psychiatry, and other disciplines is on the horizon as liquid biopsy uncovers molecular nuances indicative of disease emergence or progression.…”

Section: Future D Irec Ti On In Multiflu Id B Iops Ie Smentioning

confidence: 99%

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

Bao,

Zhang,

Guo

et al. 2024

Cancer Science

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Liquid biopsy is emerging as a pivotal tool in precision oncology, offering a noninvasive and comprehensive approach to cancer diagnostics and management. By harnessing biofluids such as blood, urine, saliva, cerebrospinal fluid, and pleural effusions, this technique profiles key biomarkers including circulating tumor DNA, circulating tumor cells, microRNAs, and extracellular vesicles. This review discusses the extended scope of liquid biopsy, highlighting its indispensable role in enhancing patient outcomes through early detection, continuous monitoring, and tailored therapy. While the advantages are notable, we also address the challenges, emphasizing the necessity for precision, cost‐effectiveness, and standardized methodologies in its broader application. The future trajectory of liquid biopsy is set to expand its reach in personalized medicine, fueled by technological advancements and collaborative research.

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Investigation of liquid biopsy analytes in peripheral blood of individuals after SARS-CoV-2 infection

Cited by 6 publications

References 34 publications

Comparison of immune cell profiles associated with heatstroke, sepsis, or cardiopulmonary bypass: Study protocol for an exploratory, case-control study trial

Comparison of immune cell profiles associated with heatstroke, sepsis, or cardiopulmonary bypass: Study protocol for an exploratory, case-control study trial

Intrinsic factors behind long COVID: III. Persistence of SARS‐CoV‐2 and its components

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

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