Investigation of miR-136-5p key target genes and pathways in lung squamous cell cancer based on TCGA database and bioinformatics analysis

Chen, Gang; Li, Tiantian; Gan, Bin‐Liang; Gao, Xiang; Li, Gao; Hu, Xiaohua

doi:10.1016/j.prp.2018.03.028

Cited by 39 publications

(35 citation statements)

References 45 publications

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“…The previous works have identified multiplies miR-136-5p targets including UGT1A7, ADH7, and ROCK1 in cancers. 17,18 Next, we found CBX4 as a target for miR-136-5p. CBX4 belongs to the Polycomb group (PcG) family and revealed to promote lung cancer proliferation and metastasis through recruiting BMI-1, indicating CBX4 may be a possible therapeutic target in cancer.…”

Section: Discussionmentioning

confidence: 86%

“…16 In addition, miR-136-5p was found decreased expression in lung squamous cell cancer and it may interact with UGT1A7 and ADH7 to regulate cellular metabolism processes. 17 We validated the direct connection of FOXP4-AS1 and miR-136-5p using luciferase activity reporter assay. The previous works have identified multiplies miR-136-5p targets including UGT1A7, ADH7, and ROCK1 in cancers.…”

Section: Discussionmentioning

confidence: 97%

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<p>LncRNA FOXP4-AS1 Is Involved in Cervical Cancer Progression via Regulating miR-136-5p/CBX4 Axis</p>

Zhao¹,

Yang²,

Long³

2020

OTT

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Introduction: Cervical cancer (CC) is a major health threat to women worldwide. Long non-coding RNA (lncRNA) has been reported to play crucial roles in regulating carcinogenesis, including CC. Methods: In this work, levels of lncRNA forkhead box P4 antisense RNA 1 (FOXP4-AS1) in CC cell lines and normal cell lines were analyzed with quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) method. Effects of FOXP4-AS1 on CC cellular behaviors including proliferation, migration, and invasion were explored. Bioinformatic prediction tools and luciferase activity reporter assay were conducted to explore the downstream molecules for FOXP4-AS1. Results: We found FOXP4-AS1 expression was significantly higher in CC cell lines than in normal cell line. Functionally, force FOXP4-AS1 expression increased CC cell proliferation, migration, and invasion, while FOXP4-AS1 knockdown caused opposite effects. Mechanistically, we found FOXP4-AS1 acts as competing endogenous RNA (ceRNA) for microRNA-136-5p (miR-136-5p) to regulate chromobox 4 (CBX4) expression. Discussion: These findings indicated FOXP4-AS1 plays an oncogenic role in CC, which may provide novel therapeutic biomarker against CC.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 97%

<p>LncRNA FOXP4-AS1 Is Involved in Cervical Cancer Progression via Regulating miR-136-5p/CBX4 Axis</p>

Zhao¹,

Yang²,

Long³

2020

OTT

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“…Yan et al (2016) have shown miR‐136 inhibits the malignant process via RASAL2 in triple‐negative breast cancer. Xie et al (2018) recently demonstrated that miR‐136‐5p expression is downregulated in LUSC tissues. In our study, miR‐136‐5p also had a suppressive role on LUSC, which is consistent with existing research.…”

Section: Discussionmentioning

confidence: 99%

CircTIMELESS regulates the proliferation and invasion of lung squamous cell carcinoma cells via the miR‐136‐5p/ROCK1 axis

Zhang

Shi

Chuan-hao

et al. 2020

Journal Cellular Physiology

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Numerous studies demonstrate that circular RNAs (circRNAs) are critical regulators of the occurrence and progression of tumors. However, research on the involvement of circRNAs in lung squamous cell carcinoma (LUSC) is limited. In our study, circTIMELESS (also named hsa_circ_0000408 in the Human circRNA Database) was upregulated in both LUSC tissues and LUSC cells, and circTIMELESS expression was positively associated with the TNM stage. Moreover, circTIMELESS silencing markedly suppressed invasion in vitro and disrupted proliferation in vitro as well as in vivo. Additional investigations have shown that circTIMELESS functions as a miR-136-5p "sponge" and regulates miR-136-5p expression. Furthermore, the impact of miR-136-5p upregulation was consistent with the results of circTIMELESS silencing, both of which inhibited the proliferation and invasion of LUSC cells.Additional results showed that Rho-associated coiled-coil containing protein kinase 1 (ROCK1) is targeted by miR-136-5p. The results of recovery experiments showed that ROCK1 overexpression partly rescued the impact of circTIMELESS silencing and miR-136-5p upregulation on proliferation and invasion. Consequently, our findings confirmed that circTIMELESS exists in LUSC and acts as a tumor promoter through the miR-136-5p/ROCK1 axis. Based on these findings, circTIMELESS may be potentially utilized as a therapeutic target for LUSC. K E Y W O R D S circTIMELESS, lung squamous cell carcinoma, miR-136-5p, ROCK1 CircTIMELESS regulates the proliferation and invasion of lung squamous cell carcinoma cells via the miR-136-5p/ROCK1 axis.

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“…Samples from patients who had received radiotherapy or chemotherapy prior to their surgery were excluded from the present study. To study the expression of GLTSCR1 at the mRNA level and complete a survival analysis, clinicopathological features from TCGA database, a freely opened public platform, is a source for abundant cancer-related data (19), including 499 prostate cancer tissues and 52 normal prostate tissues, were also gathered. These features are summarized in Table I.…”

Section: Methodsmentioning

confidence: 99%

High levels of glioma tumor suppressor candidate region gene 1 predicts a poor prognosis for prostate cancer

Zhu

et al. 2018

Oncol Lett

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Glioma tumor suppressor candidate region gene 1 (GLTSCR1) is associated with the progression of oligodendroglioma. However, there has been little study of GLTSCR1 in prostate cancer. In the present study, the association between the expression of GLTSCR1, and the progression and prognosis of tumors in patients with prostate cancer was assessed. An immunohistochemical analysis was performed using a human tissue microarray for GLTSCR1 at the protein expression level and the immunostaining results were evaluated against clinical variables of patients with prostate cancer. Subsequently, The Cancer Genome Atlas (TCGA) was used to validate the analysis results at the mRNA level and to study the prognostic value of GLTSCR1 in prostate cancer. Immunohistochemistry and TCGA data analysis revealed that GLTSCR1 expression in the prostate cancer tissues was significantly higher than that in the benign prostate tissues (immunoreactivity score, P=0.015; mRNA levels: cancer, 447.7±6.45 vs. benign, 343.5±4.21; P<0.001). Additionally, the increased GLTSCR1 protein expression was associated with certain clinical variables in the prostate cancer tissues, including advanced clinical stage (P<0.001), enhanced tumor invasion (P=0.003), lymph node metastasis (P=0.003) and distant metastasis (P=0.001). TCGA data revealed similar results, demonstrating that the upregulation of GLTSCR1 mRNA expression was associated with the Gleason score (P<0.001), enhanced tumor invasion (P=0.011), lymph node metastasis (P=0.001) and distant metastasis (P=0.002). Furthermore, Kaplan-Meier analysis suggested that among all patients, high GLTSCR1 expression indicated a decreased overall survival (P=0.028) and biochemical recurrence (BCR)-free survival (P=0.004), compared with patients with low GLTSCR1 expression. Finally, multivariate analysis revealed that the expression of GLTSCR1 was an independent predictor of poor BCR-free survival (P=0.049). The present study suggested that the increased expression of GLTSCR1 was associated with the progression of prostate cancer. Furthermore, GLTSCR1 may be a novel biomarker that is able to predict the clinical outcome in prostate cancer patients.

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Investigation of miR-136-5p key target genes and pathways in lung squamous cell cancer based on TCGA database and bioinformatics analysis

Cited by 39 publications

References 45 publications

<p>LncRNA FOXP4-AS1 Is Involved in Cervical Cancer Progression via Regulating miR-136-5p/CBX4 Axis</p>

<p>LncRNA FOXP4-AS1 Is Involved in Cervical Cancer Progression via Regulating miR-136-5p/CBX4 Axis</p>

CircTIMELESS regulates the proliferation and invasion of lung squamous cell carcinoma cells via the miR‐136‐5p/ROCK1 axis

High levels of glioma tumor suppressor candidate region gene 1 predicts a poor prognosis for prostate cancer

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