Investigation of Phytocomponents and Hypoglycaemic Effect of Hydro-methanolic Leaf Extract of Cnidoscolus aconitifolius(Spinach Tree) in Streptozotocin Induced - Diabetic Wistar Rats

Iyke, Weleh Ikechukwu; Nwafor, A; Njoku, Bestman; Uchenna, Nwoke Kyrian; Numbara, Deebii

doi:10.9734/jocamr/2018/26858

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…According to the grouping, animals in group 1 served as the Negative Control group and only received distilled water while those in groups 2 and 3 which served as the Extract Treatment groups received 200 and 400 mg/kg BW of the HMLECA respectively. The entire administration was by oral gavage once daily for 58 days, while the dose of the extract used was based on that of Iyke et al, 22 and the toxicity (LD50) based on the findings of Adebiyi et al 6…”

Section: Experimental Animal and Designmentioning

confidence: 99%

Hepato-Renal Activities of Hydro-Methanol Leaf Extract of Cnidoscolus aconitifolius in Adult Male Wistar Rats

Ezebuiro

Ododo

2021

J. Drug Delivery Ther.

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Introduction: Medicinal plants such as Cnidoscolus aconitifolius (CA) have been studied over the years for their protective and curative potentials against a myriad of common global health challenges such as hepatorenal injuries. Objectives: To ascertaining the effects of the hydromethanol leaf extract of CA (HMLECA) on hepato-renal parameters in adult male wistar rats. Method: A total of 18 adult male wistar rats were divided into 3 groups of six rats each. Group I served as the negative control which received distilled water while groups 2 and 3 served as extract treatment groups which received 200 and 400 mg/kg BW of the HMLECA respectively. The administration was daily for a period of 58 days while blood sample for the biochemical analysis was drawn via cardiac puncture at the end of the study following light chloroform anaesthesia. Results and Discussion: The results showed that the administration of both doses of the extract produced no significant (P<0.05) effect on the levels of serum liver enzyme (AST, ALT, ALP), TP, ALB, electrolytes (K+, Na+ and HCO3–), Creatinine and Urea while that of the 400 mg/kg BW produced significant (P<0.05) decrease in the level of TB and Cl –. Hence, the administration of the leaf extract in this study did not elicit any toxic effect on both the liver and kidney functions and could be effective as hepato-renal protective agent. Keywords: Cnidoscolus aconitifolius, Liver function, Liver enzymes, Renal function, Serum biochemicals, Serum electrolytes

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Section: Experimental Animal and Designmentioning

confidence: 99%

Hepato-Renal Activities of Hydro-Methanol Leaf Extract of Cnidoscolus aconitifolius in Adult Male Wistar Rats

Ezebuiro

Ododo

2021

J. Drug Delivery Ther.

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“…Adeniran et al [ 10 ] showed that the ethanolic extracts of its roots, stems, and leaves had obvious inhibitory effects on Bacillus subtilis and Escherichia coli , and the antibacterial effects of all extracts were better than those of some common commercial antibiotics, such as tetracycline. C. aconitifolius extracts also showed the potential to lower blood sugar, protect the stomach, prevent precancerous lesions of the colon in rats, and protect the liver [ 11 , 12 , 13 , 14 ]. In addition, the addition of C. aconitifolius pellets to conventional feeds can significantly increase the nutrient content and crude protein digestibility of feeds [ 15 , 16 , 17 ], which can help to solve the world feed resource shortage problem.…”

Section: Introductionmentioning

confidence: 99%

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Jiang

et al. 2022

Plants

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Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (p < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.

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Renal and Hepatic Disease:Cnidoscolus aconitifoliusas Diet Therapy Proposal for Prevention and Treatment

Valdez

2020

Journal of the American College of Nutrition

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Investigation of Phytocomponents and Hypoglycaemic Effect of Hydro-methanolic Leaf Extract of Cnidoscolus aconitifolius(Spinach Tree) in Streptozotocin Induced - Diabetic Wistar Rats

Cited by 4 publications

References 9 publications

Hepato-Renal Activities of Hydro-Methanol Leaf Extract of Cnidoscolus aconitifolius in Adult Male Wistar Rats

Hepato-Renal Activities of Hydro-Methanol Leaf Extract of Cnidoscolus aconitifolius in Adult Male Wistar Rats

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Renal and Hepatic Disease:Cnidoscolus aconitifoliusas Diet Therapy Proposal for Prevention and Treatment

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