Investigation of siRNA Nanoparticle Formation Using  Mono-Cationic Detergents and Its Use in Gene Silencing in Human HeLa Cells

Yamada, Yuma; Suzuki, Ryosuke; Harashima, Hideyoshi

doi:10.3390/cancers5041413

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…To date, we have been successful in efficiently packaging nucleic acids, including oligo DNA [13,14] and siRNA [15,16], and showed that the MEND system achieved efficient gene silencing and RNA knockdown [14][15][16][17][18][19]. These results prompted us to consider the possibility that a MEND system could be used to achieve the efficient cytosolic delivery of nucleic acids targeted to impregnable MIN6 cells.…”

Section: Introductionmentioning

confidence: 99%

A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

et al. 2014

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Pancreatic β cells secrete insulin in response to glucose levels and thus are involved in controlling blood glucose levels. A line of pancreatic β cells "MIN6" has been used in studies related to the function of β cells and diabetes therapy. Regulating gene expression in MIN6 cells could accelerate these studies, but an efficient method for the transfection of nucleic acids targeted to MIN6 cells is required. We report here on a liposome-based carrier targeted to pancreatic β cells (Multifunctional envelope-type nano device for pancreatic β cells, β-MEND). We identified a lipid composition for use in preparing the β-MEND, which permits the particles to be efficiently internalized into MIN6, as evidenced by flow cytometry analyses. Intracellular observation by confocal laser scanning microscopy showed that the β-MEND efficiently delivered the oligo nucleic acids to the cytosol of MIN6 cells. Moreover, using a β-MEND encapsulating a 2'-O-Methyl RNA complementary to a microRNA that suppresses insulin secretion, the knockdown of the targeted microRNA and an up-regulation of insulin secretion were observed in MIN6. Thus, the β-MEND holds promise as an efficient system for delivering nucleic acids targeted to MIN6 and can contribute to research and therapy aimed at diabetes.

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Section: Introductionmentioning

confidence: 99%

A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

et al. 2014

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“…The percentage of gene silencing efficiency (%) of ticks injected with longicin dsRNA compared to Luc dsRNA-injected ticks was calculated as follows: Gene silencing efficiency (%) = (1–L Long /L Luc ) × 100. Where, L Long and L Luc represent the normalized longicin expressions of longicin dsRNA-injected and Luc dsRNA-injected ticks, respectively [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus

et al. 2016

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BackgroundLongicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat virus (LGTV), a tick-borne flavivirus.MethodsLongicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and virus yield. Additionally, longicin P4 was also tested for its activity against human adenovirus, a non-enveloped virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed.ResultsLongicin P4 produced significant viral foci reduction and lower virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenovirus. In addition, longicin-silenced ticks showed significantly higher virus titer after 7 days post-infection but a significantly lower titer was detected after an additional 14 days of observation as compared to the Luc dsRNA-injected ticks. Mortality in both groups did not show any significant difference.ConclusionOur results suggest that longicin P4 has in vitro antiviral activity against LGTV but not against a non-enveloped virus such as adenovirus. Likewise, though most cationic antimicrobial peptides like longicin act directly on target membranes, the exact mechanism of membrane targeting of longicin P4 in enveloped viruses, such as LGTV, requires further investigation. Lastly, while the in vitro virucidal capacity of longicin P4 was confirmed in this study, the role of the endogenous tick longicin in the antiviral defense of H. longicornis against LGTV still remains to be demonstrated.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1344-5) contains supplementary material, which is available to authorized users.

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“…Inorganic functional nanomaterials have emerged as reliable and adaptable nano scaffolds for gene delivery [ 1 , 2 ]. The synthesis of numerous primary proteins in microbes and living cells is the focus of cutting-edge recombinant techniques that have replaced outdated methods and the current trends in bio-nanotechnology.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle-Mediated Gene Therapy

Kanu

Parambath

Odeh

et al. 2022

Cancers

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Gold nanoparticles (AuNPs) have gained increasing attention as novel drug-delivery nanostructures for the treatment of cancers, infections, inflammations, and other diseases and disorders. They are versatile in design, synthesis, modification, and functionalization. This has many advantages in terms of gene editing and gene silencing, and their application in genetic illnesses. The development of several techniques such as CRISPR/Cas9, TALEN, and ZFNs has raised hopes for the treatment of genetic abnormalities, although more focused experimentation is still needed. AuNPs, however, have been much more effective in trending research on this subject. In this review, we highlight recently well-developed advancements that are relevant to cutting-edge gene therapies, namely gene editing and gene silencing in diseases caused by a single gene in humans by taking an edge of the unique properties of the AuNPs, which will be an important outlook for future research.

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Investigation of siRNA Nanoparticle Formation Using Mono-Cationic Detergents and Its Use in Gene Silencing in Human HeLa Cells

Cited by 4 publications

References 26 publications

A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus

Gold Nanoparticle-Mediated Gene Therapy

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