Investigation of the elution behavior of dissociating itaconic acid on a hydrophobic polymeric adsorbent using in-line Raman spectroscopy

“…Most of the itaconic acid puri cation techniques require the free acid. Therefore, the fully protonated state is required to obtain high yields during DSP (14,21,(23)(24)(25)(26). As a result, the pH needs to be lowered after fermentation by acid addition.…”

Section: Introductionmentioning

confidence: 99%

Balancing pH and Yield: Exploring Itaconic Acid Production in Ustilago cynodontis from an Economic Perspective

Ernst,

Saur,

Kiefel

et al. 2024

Preprint

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Background: Itaconic acid is a promising bio-based building block for the synthesis of polymers, plastics, fibers and other materials. In recent years, Ustilago cynodontis has emerged as an additional itaconate producing non-conventional yeast, mainly due to its high acid tolerance, which significantly reduces saline waste coproduction during fermentation and downstream processing. As a result, this could likely improve the economic viability of the itaconic acid production process with Ustilaginaceae. Results: In this study, we characterized a previously engineered itaconate hyper-producing Ustilago cynodontis strain in controlled fed-batch fermentations to determine the minimal and optimal pH for itaconate production. Under optimal fermentation conditions, the hyper-producing strain can achieve the theoretical maximal itaconate yield during the production phase in a low-density fermentation at pH 3.6, but at the expense of considerable base addition. Base consumption is strongly reduced at the pH of 2.8, but at cost of production yield, titer, and rate. A techno-economic analysis based on the entire process demonstrated that savings due to an additional decrease in pH control reagents and saline waste costs cannot compensate the yield loss observed at the highly acidic pH value 2.8. Conclusions: Overall, this work provides novel data regarding the individual strain properties and production capabilities, contributing to a better understanding of the itaconic acid production process with Ustilago cynodontis, especially from an economic perspective.

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Model-based investigation of the pH-dependent chromatographic separation of itaconic acid from aqueous solution using strongly hydrophobic adsorbents

Biselli,

Reifsteck,

Tesanovic

et al. 2024

Journal of Chromatography A

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Investigation of the elution behavior of dissociating itaconic acid on a hydrophobic polymeric adsorbent using in-line Raman spectroscopy

Cited by 5 publications

References 30 publications

Review of quantitative and qualitative methods for monitoring photopolymerization reactions

Review of quantitative and qualitative methods for monitoring photopolymerization reactions

Balancing pH and Yield: Exploring Itaconic Acid Production in Ustilago cynodontis from an Economic Perspective

Model-based investigation of the pH-dependent chromatographic separation of itaconic acid from aqueous solution using strongly hydrophobic adsorbents

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