Investigation of the recognition interaction between glycated hemoglobin and its aptamer by using surface plasmon resonance

Sun, Dalin; Wu, Yi Xi; Chang, Shwu-Jen; Liu, Jen‐Tsai

doi:10.1016/j.talanta.2020.121466

Cited by 26 publications

(13 citation statements)

References 44 publications

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“…We note that the K D values found for our engineered GHA immobilized on the Type-1 and Type-2 surfaces are similar to those for gHb-targeted aptamer reported in other works. ,,, However, the K D value for our Type-3 surface, 0.32 nM, is substantially lower than many values reported in literature for gHb-targeted aptamers. − Taken together and in the context of previous literature, our results support the assertion that the inclusion of 11MUA and DMOL surface modifications into aptamer-based SPR biosensing surfaces can improve the inherent specific affinities engineered into aptamers, through their aptamer enhancing and antifouling properties.…”

Section: Resultssupporting

confidence: 90%

Adsorption Kinetics of Glycated Hemoglobin on Aptamer Microarrays with Antifouling Surface Modification

et al. 2021

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Aptamers are oligonucleotides that bind with high affinity to target molecules of interest. One such target is glycated hemoglobin (gHb), a biomarker for assessing glycemic control and diabetes diagnosis. By the coupling of aptamers with surface plasmon resonance (SPR) sensing surfaces, a fast, reliable and inexpensive assay for gHb can be developed. In this study, we tested the affinity of SPR-sensing surfaces, composed of aptamers and antifouling self-assembled monolayers (SAMs), to hemoglobin (Hb) and gHb. First, we developed a gHb-targeted aptamer (GHA) through a modified Systematic Evolution of Ligands by EXponential (SELEX) enrichment process and tested its affinity to gHb using the Nano-Affi protocol. GHA was used to produce three distinct SAM-SPR-sensing surfaces: (Type-1) a SAM of GHA directly attached to a sensor surface; (Type-2) GHA attached to a SAM of 11-mercaptoundecanoic acid (11MUA) on a sensor surface; (Type-3) GHA attached to a binary SAM of 11MUA and 3,6-dioxa-8-mercaptooctan-1-ol (DMOL) on a sensor surface. Type-2 and Type-3 surfaces were characterized by cyclic voltammetry and electrochemical impedance spectroscopy to confirm that GHA bound to the underlying SAMs. The adsorption kinetics for Hb and gHb interacting with each SPR sensing surface were used to quantify their respective affinities. The Type-1 surface without antifouling modification had a dissociation constant ratio (K D,Hb / K D,gHb ) of 9.7, as compared to 809.3 for the Type-3 surface, demonstrating a higher association of GHA to gHb for sensor surfaces with antifouling modifications than those without. The enhanced selectivity of GHA to gHb can likely be attributed to the inclusion of DMOL in the SAM-modified surface, which reduced interference from nonspecific adsorption of proteins. Results suggest that pairing aptamers with antifouling SAMs can significantly improve their target affinity, potentially allowing for the development of novel, low cost, and fast assays.

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Section: Resultssupporting

confidence: 90%

Adsorption Kinetics of Glycated Hemoglobin on Aptamer Microarrays with Antifouling Surface Modification

et al. 2021

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“…Surface plasmon resonance (SPR) is a well-known sensing technology used for characterising kinetics of ligand–receptor interactions and these types of sensors offer unique real-time and label-free measurement capabilities with high detection sensitivity [ 44 , 45 , 46 ]. Figure 2 A shows a typical SPR biosensor configuration where biorecognition elements, such as antibodies, are immobilised on the surface of a thin gold film.…”

Section: Latest Developed Biosensors For Covid-19mentioning

confidence: 99%

Emerging Biosensors to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A Review

2021

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Coronavirus disease (COVID-19) is a global health crisis caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) is the gold standard test for diagnosing COVID-19. Although it is highly accurate, this lab test requires highly-trained personnel and the turn-around time is long. Rapid and inexpensive immuno-diagnostic tests (antigen or antibody test) are available, but these point of care (POC) tests are not as accurate as the RT-PCR test. Biosensors are promising alternatives to these rapid POC tests. Here we review three types of recently developed biosensors for SARS-CoV-2 detection: surface plasmon resonance (SPR)-based, electrochemical and field-effect transistor (FET)-based biosensors. We explain the sensing principles and discuss the advantages and limitations of these sensors. The accuracies of these sensors need to be improved before they could be translated into POC devices for commercial use. We suggest potential biorecognition elements with highly selective target-analyte binding that could be explored to increase the true negative detection rate. To increase the true positive detection rate, we suggest two-dimensional materials and nanomaterials that could be used to modify the sensor surface to increase the sensitivity of the sensor.

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“…Yet, in certain cases, the target itself has been shown to adsorb onto the nanostructures. For instance, Ochratoxin A (OTA), one of the most common food contaminants, an extremely toxic molecule for living organisms, can also adsorb onto AuNPs, thus making accurate detection difficult [ 71 , 72 ]. This phenomenon was first described by Liu et al, during their attempt to widen the detection range of OTA by doubling the calibration curve of their gold NP aptamer-based localized surface plasmon resonance (LSPR) sensor [ 71 ].…”

Section: Aptameric Structure-based Plasmon Biosensormentioning

confidence: 99%

Recent Advances in DNA Nanotechnology for Plasmonic Biosensor Construction

et al. 2022

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Since 2010, DNA nanotechnology has advanced rapidly, helping overcome limitations in the use of DNA solely as genetic material. DNA nanotechnology has thus helped develop a new method for the construction of biosensors. Among bioprobe materials for biosensors, nucleic acids have shown several advantages. First, it has a complementary sequence for hybridizing the target gene. Second, DNA has various functionalities, such as DNAzymes, DNA junctions or aptamers, because of its unique folded structures with specific sequences. Third, functional groups, such as thiols, amines, or other fluorophores, can easily be introduced into DNA at the 5′ or 3′ end. Finally, DNA can easily be tailored by making junctions or origami structures; these unique structures extend the DNA arm and create a multi-functional bioprobe. Meanwhile, nanomaterials have also been used to advance plasmonic biosensor technologies. Nanomaterials provide various biosensing platforms with high sensitivity and selectivity. Several plasmonic biosensor types have been fabricated, such as surface plasmons, and Raman-based or metal-enhanced biosensors. Introducing DNA nanotechnology to plasmonic biosensors has brought in sight new horizons in the fields of biosensors and nanobiotechnology. This review discusses the recent progress of DNA nanotechnology-based plasmonic biosensors.

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Investigation of the recognition interaction between glycated hemoglobin and its aptamer by using surface plasmon resonance

Cited by 26 publications

References 44 publications

Adsorption Kinetics of Glycated Hemoglobin on Aptamer Microarrays with Antifouling Surface Modification

Adsorption Kinetics of Glycated Hemoglobin on Aptamer Microarrays with Antifouling Surface Modification

Emerging Biosensors to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A Review

Recent Advances in DNA Nanotechnology for Plasmonic Biosensor Construction

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