Involvement of Hydrogen Peroxide in Cotton Leaf Abscission Induced by Thidiazuron

A LcDOF5.6–LcRbohD regulatory module controls the reactive oxygen species‐mediated fruitlet abscission in litchi

“…Before organ abscission, ROS accumulation has been found in leaf AZ of several species. For instance, ROS accumulation in leaf AZ was found in ethephon‐induced leaf abscission in olive and thidiazuron‐induced leaf abscission in cotton (Goldental‐Cohen et al., 2017; Li et al., 2020). In this study, we determined the dynamics of ROS accumulation in FAZ during the GPD‐induced fruitlet abscission, and found that ROS accumulation in FAZ was constantly enhanced after GPD treatment (Figure 1).…”

“…In Arabidopsis, genetic evidence indicated that the NADPH oxidases RbohD and RbohF are involved in lignin formation, which produces a honeycomb structure acting as a mechanical “brace” to localize cell wall breakdown and required for precise floral organ abscission (Lee et al., 2018). In cotton, it was reported that thidiazuron‐induced leaf abscission is associated with the H 2 O 2 production in leaf, petiole, and AZ, probably due to the induced expression of RbohA (Li et al., 2020). However, the mechanism underlying how RBOHs are regulated in the process of organ abscission remains unknown.…”

“…Increasing evidence has shown that ROS plays a critical role in regulating organ abscission. First, ROS accumulation has been reported during abscission in various species, such as the pedicel abscission in tobacco, leaf abscission in pepper, olive, cassava, and cotton, and fruitlet abscission in apple (Botton et al., 2011; Eccher et al., 2013; Goldental‐Cohen et al., 2017; Henry et al., 1974; Li et al., 2020; Liao et al., 2016; Sakamoto et al., 2008a). Secondly, it was found that the application of ROS inducers or scavengers could either accelerate or decelerate, respectively, the process of organ abscission (Li et al., 2020; Liao et al., 2016; Sakamoto et al., 2008a).…”

“…First, ROS accumulation has been reported during abscission in various species, such as the pedicel abscission in tobacco, leaf abscission in pepper, olive, cassava, and cotton, and fruitlet abscission in apple (Botton et al., 2011; Eccher et al., 2013; Goldental‐Cohen et al., 2017; Henry et al., 1974; Li et al., 2020; Liao et al., 2016; Sakamoto et al., 2008a). Secondly, it was found that the application of ROS inducers or scavengers could either accelerate or decelerate, respectively, the process of organ abscission (Li et al., 2020; Liao et al., 2016; Sakamoto et al., 2008a). Furthermore, genes responsible for ROS metabolism are found to be preferentially expressed in AZ cells, and are required to fine‐tune the timing and precisely accomplish the floral organ abscission in Arabidopsis (Y. Lee et al., 2018; J. Lee et al., 2022).…”

The effect of cytokinins on shoot proliferation, biochemical changes and genetic stability of Rhododendron ‘Kazimierz Odnowiciel’ in the in vitro cultures

“…In the case of TDZ it is a frequent plant response due to strong action of this synthetic cytokinin. At first, TDZ was used as a defoliant, the recent investigations show that its action is associated with stress and increased H 2 O 2 concentration in plant cells what results in leaf abscission (Li et al 2020). A high hydrogen concentration is harmful as it damages structural cell elements due to their oxidation and degradation what in consequence provokes death of cells and the whole organism.…”