Involvement of in vivo induced <i>cheY‐4</i> gene of <i>Vibrio cholerae</i> in motility, early adherence to intestinal epithelial cells and regulation of virulence factors

Banerjee, Rajat; Das, Soumita; Mukhopadhyay, Kanchan; Nag, Sanjay; Chakrabortty, Amit; Chaudhuri, Keya

doi:10.1016/s0014-5793(02)03678-5

Cited by 27 publications

(29 citation statements)

References 22 publications

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“…Another possibility is that the modulated chemotaxis is due to varied gene expression regulated by CheY4. Indeed, the duplication of cheY4 increases the expression levels of ctxAB and tcpA (3). It has also been reported that motility and the expression of ctxAB are inversely regulated by bile (13).…”

Section: Discussionmentioning

confidence: 94%

“…Nonchemotactic mutants with counterclockwise-and clockwise-biased flagellar rotation were reported to show enhanced and attenuated infectivity, respectively (6). In an in vitro study (3), cells lacking or overproducing CheY4 exhibited a decreased or increased production of CT, respectively, and CheY4 was suggested to be involved in attachment to host cell layers and migration toward ingredients of the intestine. It was also reported that two MLPs (TcpI and AcfB) are involved in colonization (9,15) and that another MLP (HlyB) is involved in the secretion of hemolysin (1,18).…”

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confidence: 99%

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Only One of the Five CheY Homologs in Vibrio cholerae Directly Switches Flagellar Rotation

Hyakutake¹,

Homma²,

Austin

et al. 2005

J Bacteriol

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Vibrio cholerae has three sets of chemotaxis (Che) proteins, including three histidine kinases (CheA) and four response regulators (CheY) that are encoded by three che gene clusters. We deleted the cheY genes individually or in combination and found that only the cheY3 deletion impaired chemotaxis, reinforcing the previous conclusion that che cluster II is involved in chemotaxis. However, this does not exclude the involvement of the other clusters in chemotaxis. In other bacteria, phospho-CheY binds directly to the flagellar motor to modulate its rotation, and CheY overexpression, even without CheA, causes extremely biased swimming behavior. We reasoned that a V. cholerae CheY homolog, if it directly controls flagellar rotation, should also induce extreme swimming behavior when overproduced. This was the case for CheY3 (che cluster II). However, no other CheY homolog, including the putative CheY (CheY0) protein encoded outside the che clusters, affected swimming, demonstrating that these CheY homologs cannot act directly on the flagellar motor. CheY4 very slightly enhanced the spreading of an Escherichia coli cheZ mutant in semisolid agar, raising the possibility that it can affect chemotaxis by removing a phosphoryl group from CheY3. We also found that V. cholerae CheY3 and E. coli CheY are only partially exchangeable. Mutagenic analyses suggested that this may come from coevolution of the interacting pair of proteins, CheY and the motor protein FliM. Taken together, it is likely that the principal roles of che clusters I and III as well as cheY0 are to control functions other than chemotaxis.

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Only One of the Five CheY Homologs in Vibrio cholerae Directly Switches Flagellar Rotation

Hyakutake¹,

Homma²,

Austin

et al. 2005

J Bacteriol

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“…The bacterium inhabits nutrient-poor aquatic environments such as rivers and estuaries or occasionally intrudes into the nutrient-rich lumen of the human gastrointestinal tract (38). Chemotaxis of V. cholerae toward favorable locations is proposed to play critical roles in its survival (6) and pathogenicity (2,5,8,13,14,15,16,17,20,21,30,31). However, the external signals and cellular factors that mediate chemotaxis of V. cholerae are only poorly understood.…”

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confidence: 99%

Mlp24 (McpX) of Vibrio cholerae Implicated in Pathogenicity Functions as a Chemoreceptor for Multiple Amino Acids

et al. 2012

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ABSTRACTThe chemotaxis ofVibrio cholerae, the causative agent of cholera, has been implicated in pathogenicity. The bacterium has more than 40 genes for methyl-accepting chemotaxis protein (MCP)-like proteins (MLPs). In this study, we found that glycine and at least 18l-amino acids, including serine, arginine, asparagine, and proline, serve as attractants to the classical biotype strain O395N1. Based on the sequence comparison withVibrio parahaemolyticus, we speculated that at least 17 MLPs ofV. choleraemay mediate chemotactic responses. Among them, Mlp24 (previously named McpX) is required for the production of cholera toxin upon mouse infection.mlp24deletion strains of both classical and El Tor biotypes showed defects in taxis toward several amino acids, which were complemented by the expression of Mlp24. These amino acids enhanced methylation of Mlp24. Serine, arginine, asparagine, and proline were shown to bind directly to the periplasmic fragment of Mlp24. The structural information of its closest homolog, Mlp37, predicts that Mlp24 has two potential ligand-binding pockets per subunit, the membrane distal of which was suggested, by mutational analyses, to be involved in sensing of amino acids. These results suggest that Mlp24 is a chemoreceptor for multiple amino acids, including serine, arginine, and asparagine, which were previously shown to stimulate the expression of several virulence factors, implying that taxis toward a set of amino acids plays critical roles in pathogenicity ofV. cholerae.

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“…Phospho-CheY then interacts with the C-ring of the flagellum, which causes a reversion from CCW to CW rotation, resulting in a change of swimming direction. CheB and CheW are involved in modulating the signal transduction pathway (Freter and O'Brien 1981;Alm and Manning 1990;Everiss, Hughes et al 1994;Harkey, Everiss et al 1994;Lee, Butler et al 2001;Banerjee, Das et al 2002;Hyakutake, Homma et al 2005). …”

Section: Chemotaxis and Virulencementioning

confidence: 99%

Vibrio cholerae Flagellar Synthesis and Virulence

Anastasia¹,

Karl²

2012

Cholera

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Involvement of in vivo induced cheY‐4 gene of Vibrio cholerae in motility, early adherence to intestinal epithelial cells and regulation of virulence factors

Cited by 27 publications

References 22 publications

Only One of the Five CheY Homologs in Vibrio cholerae Directly Switches Flagellar Rotation

Only One of the Five CheY Homologs in Vibrio cholerae Directly Switches Flagellar Rotation

Mlp24 (McpX) of Vibrio cholerae Implicated in Pathogenicity Functions as a Chemoreceptor for Multiple Amino Acids

Vibrio cholerae Flagellar Synthesis and Virulence

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