Involvement of Intracellular Calcium in Anaerobic Gene Expression and Survival of Maize Seedlings

Subbaiah, Chalivendra C.; Zhang, Junkai; Sachs, Martin M.

doi:10.1104/pp.105.1.369

Cited by 157 publications

(108 citation statements)

References 33 publications

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“…Recent evidence indicates that anoxia somehow elicits a signal transduction pathway involving Ca2'. The anoxic induction of ADH was blocked in maize roots by an inhibitor of organellar Ca2+ fluxes (Subbaiah et al, 1994b), whereas a drug that increased cytosolic [Ca2+] caused expression of ADH in the absence of anoxia (Subbaiah et al, 1994a). Since ethylene production is known to be blocked by strict anoxia, the pathway is presumably independent of ethylene.…”

mentioning

confidence: 98%

Transduction of an Ethylene Signal Is Required for Cell Death and Lysis in the Root Cortex of Maize during Aerenchyma Formation Induced by Hypoxia

1996

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Ethylene has been implicated in signaling cell death in the lysigenous formation of gas spaces (aerenchyma) in the cortex of adventitious roots of maize (Zea mays) subjected to hypoxia. Various antagonists that are known to modify particular steps in signal transduction in other plant systems were applied at low concentrations to normoxic and hypoxic roots of maize, and the effect on cell death (aerenchyma formation) and the increase in cellulase activity that precedes the appearance of cell degeneration were measured. 60th cellulase activity and cell death were inhibited in hypoxic roots in the presence of antagonists of inositol phospholipids, CaZ+-calmodulin, and protein kinases. By contrast, there was a parallel promotion of cellulase activity and cell death in hypoxic and normoxic roots by contact with reagents that activate G-proteins, increase cytosolic CaZ+, or inhibit protein phosphatases. Most of these reagents had no effect on ethylene biosynthesis and did not arrest root extension. These results indicate that the transduction of an ethylene signal leading to an increase in intracellular Ca2+ is necessary for cell death and the resulting aerenchyma development in roots of maize subjected to hypoxia.

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confidence: 98%

Transduction of an Ethylene Signal Is Required for Cell Death and Lysis in the Root Cortex of Maize during Aerenchyma Formation Induced by Hypoxia

1996

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“…In both maize and Arabidopsis, calcium has a key role in sustaining an appropriate response to hypoxia (Subbaiah et al, 1994a(Subbaiah et al, , 1994b and is involved in regulating secondary effects of hypoxia such as aerenchyma formation and tissue necrosis (Drew et al, 2000;Subbaiah et al, 2000). Calcium has also been implicated in the signal transduction associated with gravity orientation (Reddy et al, 1987;Merkys and Darginaviciene, 1997;Kiss, 2000).…”

Section: Discussionmentioning

confidence: 99%

Transgene Expression Patterns Indicate That Spaceflight Affects Stress Signal Perception and Transduction in Arabidopsis

et al. 2001

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The use of plants as integral components of life support systems remains a cornerstone of strategies for long-term human habitation of space and extraterrestrial colonization. Spaceflight experiments over the past few decades have refined the hardware required to grow plants in low-earth orbit and have illuminated fundamental issues regarding spaceflight effects on plant growth and development. Potential incipient hypoxia, resulting from the lack of convection-driven gas movement, has emerged as a possible major impact of microgravity. We developed transgenic Arabidopsis containing the alcohol dehydrogenase (Adh) gene promoter linked to the ␤-glucuronidase (GUS) reporter gene to address specifically the possibility that spaceflight induces the plant hypoxia response and to assess whether any spaceflight response was similar to control terrestrial hypoxia-induced gene expression patterns. The staining patterns resulting from a 5-d mission on the orbiter Columbia during mission STS-93 indicate that the Adh/GUS reporter gene was activated in roots during the flight. However, the patterns of expression were not identical to terrestrial control inductions. Moreover, although terrestrial hypoxia induces Adh/GUS expression in the shoot apex, no apex staining was observed in the spaceflight plants. This indicates that either the normal hypoxia response signaling is impaired in spaceflight or that spaceflight inappropriately induces Adh/GUS activity for reasons other than hypoxia.

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“…In contrast, the activity of the 28-, 18.6-, and 10-kD RNases was stimulated in the presente of EDTA and inhibited by certain ions, indicating no ion requirement. Second, we considered that changes in Ca2+ might influence RNase activity, since a measurable increase in cytosolic Ca2+ levels occurs within 15 min of O, deprivation of maize suspension-cultured-cells (Subbaiah et al, 1994). We found that physiological concentrations of Ca2+ (0.1-1 p~) had no effect on the activity of maize RNases (data not shown), although 2 mM Ca'+ inhibited the activity of the 16.2-, 11-, and 6-kD RNases, all with a pH 6.4 optimum.…”

Section: Discussionmentioning

confidence: 99%

RNase Activities Are Reduced Concomitantly with Conservation of Total Cellular RNA and Ribosomes in O2-Deprived Seedling Roots of Maize

1997

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RNase activities are modulated during plant development and in response to certain abiotic and biotic cues and are expected to play a role in the regulation of RNA levels and turnover (for reviews, see Wilson, 1975;Farkas, 1982;Green, 1994). The electrophoretic and biochemical nature of a number of individual plant RNases and nucleases have been described, and knowledge of the function of specific RNases is slowly accumulating. The best-characterized plant RNases are the extracellular S-RNases and S-like RNases. The S-RNases are glycoproteins of the floral stigma that play a role in the gametophytic self-incompatibility mechanism in several solanaceous species. Two RNases with biochemical characteristics of S-RNases are active in developing tomato (Lycopersicon esculentum L.) fruit (McKeon et al., 1991). Phylogenetic analysis confirmed that the S-like RNases of Arabidopsis (RNS1, RNS2, and RNS3) are evolutionarily related to S-RNases, but that these enzymes do not play a role in

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Involvement of Intracellular Calcium in Anaerobic Gene Expression and Survival of Maize Seedlings

Cited by 157 publications

References 33 publications

Transduction of an Ethylene Signal Is Required for Cell Death and Lysis in the Root Cortex of Maize during Aerenchyma Formation Induced by Hypoxia

Transduction of an Ethylene Signal Is Required for Cell Death and Lysis in the Root Cortex of Maize during Aerenchyma Formation Induced by Hypoxia

Transgene Expression Patterns Indicate That Spaceflight Affects Stress Signal Perception and Transduction in Arabidopsis

RNase Activities Are Reduced Concomitantly with Conservation of Total Cellular RNA and Ribosomes in O2-Deprived Seedling Roots of Maize

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