Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae.

Bisson, Linda F.; Fraenkel, D G

doi:10.1073/pnas.80.6.1730

Cited by 300 publications

(226 citation statements)

References 28 publications

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“…This specific induction of carriers in response to glucose concentration has been found previously with stationary phase cultures of yeasts (no glucose present). In that case also carriers with higher affinity for glucose were induced (Spencer-Martins & van Uden, 1985a;van den Broek et al, 1986) (Table 3). The physiological rationale of introducing different carriers at different external glucose concentrations remains as yet unclear.…”

Section: Discussionmentioning

confidence: 99%

“…In order to avoid this problem substrate analogues have frequently been used to obtain information on the kinetics of transport in vivo (Kotyk & MichaljaniEova, 1978;Jaspers & van Steveninck, 1975;Barnett & Sims, 19763;Meredith & Romano, 1977;Franzusoff & Cirillo, 1982;van den Broek et al, 1986). Apart from the fact that such studies can only yield qualitative information with respect to the uptake of the real substrate, it must be emphasized that the results obtained with substrate analogues do not always mimic changes in the transport parameters of the real substrate (Barnett & Sims, 19763).…”

Section: Measurement Of Kinetic Parameters Of Glucose Transportmentioning

confidence: 99%

“…Previous studies have shown that hexokinases may play a role in glucose uptake in yeasts, and particularly in Saccharomyces cerevisiae (Meredith & Romano, 1977 ;Bisson & Fraenkel, 1983 …”

Section: Possible Efects Of Metabolism On Transport Parametersmentioning

confidence: 99%

“…In a variety of cases the dependence of the rate of transport on the assay sugar concentration yielded a biphasic kinetic plot indicating the presence of two uptake systems (Bisson & Fraenkel, 1983 ;Spencer-Martins & van Uden, 1985a;van den Broek et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Postma¹,

Scheffers²,

Dijken³

1988

Microbiology

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~~~ ~The relation between the kinetic parameters of glucose transport and the physiology of Candida utilis CBS 621 was studied in chemostat cultures. In glucose-limited cultures the transport parameters were dependent on the growth rate of the yeast. Three different transport systems were found which differed by an order of magnitude in their affinity constants, namely a highaffinity ( K , 25 p~), a medium-affinity ( K , 190 p~), and a low-affinity uptake system ( K , 2000 p~). Cells growing at a dilution rate of 0.45 h-l or less had the high-and medium-affinity uptake systems. At a dilution rate of 0.52 h-I the high-affinity system was absent and both the mediumand low-affinity systems were present. At a dilution rate close to pmax (0.57 h-l) only the lowaffinity system was detected. The in situ contribution of each of the transport systems to glucose consumption in glucose-limited cultures was estimated on the basis of their kinetic parameters ( K , and V,,,,,) and the residual glucose concentration in these cultures. The sum of the calculated rates of transport corresponded to the in situ rate of glucose consumption by the cultures as determined from the yield constant and the dilution rate. The dependence of the transport parameters on the growth rate and hence on the environmental sugar concentration was also evident in cells grown under nitrogen limitation. In contrast to carbon-limited cells, nitrogenlimited cultures growing at D = 0.15 h-' did not exhibit the high-affinity glucose uptake system, whereas the medium-and low-affinity systems were present.

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Section: Discussionmentioning

confidence: 99%

Section: Measurement Of Kinetic Parameters Of Glucose Transportmentioning

confidence: 99%

“…Previous studies have shown that hexokinases may play a role in glucose uptake in yeasts, and particularly in Saccharomyces cerevisiae (Meredith & Romano, 1977 ;Bisson & Fraenkel, 1983 …”

Section: Possible Efects Of Metabolism On Transport Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Postma¹,

Scheffers²,

Dijken³

1988

Microbiology

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“…At high glucose concentrations yeast exhibits lowaffinity glucose transport, and at low glucose concentrations or in the absence of glucose (e.g. after the diauxic shift of a glucose-grown batch culture) the affinity displayed by yeast for glucose is high (Bisson and Fraenkel, 1984;Walsh et al, 1994). These differences in affinity presumably reflect the substrate affinities of the individual hexose transport proteins synthesized under these conditions (Reifenberger et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Expression and activity of the Hxt7 high‐affinity hexose transporter of Saccharomyces cerevisiae

Berden

Dam

et al. 2001

Yeast

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High-affinity hexose transport is required for efficient utilization of low hexose concentrations by the baker's yeast Saccharomyces cerevisiae. These low concentrations occur during the late exponential phase of batch growth on hexoses, during hexose-limited chemostat or fed-batch culture, or during growth on sugars such as sucrose and raffinose that are hydrolysed to hexoses outside the cell. The expression of the Hxt7 high-affinity glucose transporter of S. cerevisiae was examined during batch growth on glucose medium in a wild-type strain and a strain expressing only HXT7 (i.e. with null mutations in HXT1-HXT6). In the wild-type strain, HXT7 transcription was repressed at high glucose and was detected when the glucose in the culture approached depletion. In the HXT7-only strain, transcription of HXT7 was constitutive throughout the glucose growth phase and was increased further at low glucose concentrations. After glucose depletion, the levels of HXT7 mRNA declined rapidly in both strains. In contrast, the Hxt7 protein was relatively stable after glucose depletion. By monitoring the subcellular localization of an Hxt7::GFP fusion protein it was observed that Hxt7 was localized in the plasma membrane, even when expressed at high glucose concentrations in the HXT7-only strain. After glucose depletion Hxt7 was gradually endocytosed and targeted to the vacuole for degradation. The Hxt7::GFP fusion protein was a fully functional hexose transporter with a catalytic centre activity of approximately 200/sec. It is concluded that repression of HXT7 and degradation of Hxt7 at high glucose concentrations is dependent on a high glucose transport capacity.

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High-affinity glucose uptake in Saccharomyces cerevisiae is not dependent on the presence of glucose-phosphorylating enzymes

Smits

Postma

et al. 1996

Yeast

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Glucose uptake in Saccharomyces cerevisiae is believed to consist of two kinetically distinguishable components, the affinity of which is modulated during growth on glucose. It has been reported that triple hexose‐kinase deletion mutants do not exhibit high‐affinity glucose uptake. This raises the question of whether and how high‐affinity glucose uptake is related to the presence of glucose‐phosphorylating enzymes. In this study the kinetics of glucose uptake in both wild‐type cells and cells of hexose‐kinase deletion mutants, grown on either glycerol or galactose, were determined using a rapid‐uptake method. In wild‐type cells glucose uptake measured over either 5 s or 200 ms exhibited high affinity. In contrast, in cells of hexose‐kinase deletion mutants the apparent affinity of glucose uptake was dependent on the time scale during which uptake was measured. Measurements on the 5‐s scale showed apparent low‐affinity uptake whereas measurements on the 200‐ms scale showed high‐affinity uptake. The affinity and maximal rate of the latter were comparable to those in wild‐type cells. Using a simple model for a symmetrical facilitator, it was possible to simulate the experimentally determined relation between apparent affinity and the time scale used. The results suggest that high‐affinity glucose transport is not necessarily dependent on the presence of glucose‐phosphorylating enzymes. Apparent low‐affinity uptake kinetics can arise as a consequence of an insufficient rate of removal of intracellular free glucose by phosphorylation. This study underlines the need to differentiate between influences of the translocator and of metabolism on the apparent kinetics of sugar uptake in yeast.

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Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae.

Cited by 300 publications

References 28 publications

Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Expression and activity of the Hxt7 high‐affinity hexose transporter of Saccharomyces cerevisiae

High-affinity glucose uptake in Saccharomyces cerevisiae is not dependent on the presence of glucose-phosphorylating enzymes

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