Involvement of laminin and integrins in adhesion and migration of junctional epithelium cells

Kinumatsu, Takashi; Hashimoto, Sadamitsu; Muramatsu, Takashi; Sasaki, Hodaka; Jung, Han‐Sung; Yamada, Satoru; Shimono, Masaki

doi:10.1111/j.1600-0765.2007.01036.x

Cited by 56 publications

(59 citation statements)

References 27 publications

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“…In the osteoblasts, fibronectin is primarily responsible for the adhesion between the substrate and cells 19,20) . In contrast, laminin is primarily related to the adhesion between the substrate and keratinocytes in addition to the fibronectin 21) . Another explanation may be underlying the differences in surface topography of the TZP used: a rough surface for osteoblast cultivation vs. a smooth surface for the keratinocytes in this study.…”

Section: Discussionmentioning

confidence: 99%

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

et al. 2014

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Initial attachment of human oral keratinocytes cultured on yttria-stabilized tetragonal zirconia polycrystal (TZP) surfaces that were subjected to UV or oxygen plasma (O2-plasma) treatment was investigated. The viability of the attached cells, mRNA expression of laminin γ2 and integrin β4, distribution of laminin γ2 and integrin β4, cell area, and cell morphology were assessed. The results showed that no differences in the viability of attached cells were recognized among the conditions. However, expression of laminin γ2 and integrin β4 as well as cell morphology were promoted only in O2-plasma specimens even though superhydrophilicity was obtained in both the UV and O2-plasma specimens compared with the untreated control specimen. The photocatalytic activity was believed to be closely involved in the above-mentioned differences. The results of this study suggest that TZP surface treated with oxygen plasma promotes the initial attachment capability of human oral keratinocytes with enhancing the extracellular matrix such as laminin γ2.

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Section: Discussionmentioning

confidence: 99%

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

et al. 2014

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“…The success of the transcutaneous interface around teeth and dental implants can be attributed to JE, and specifically to the α6β4 integrin component of hemidesmosomes (Masaoka et al, 2009;Oksonen et al, 2001;Shimono et al, 2003) and the laminin-332 component of the IBL (Ikeda et al, 2000;Fukano et al, 2006). The IBL has been shown to contain a 12-fold higher laminin-332 content compared with the rest of the JE (Kinumatsu et al, 2009). We hypothesised that the attachment characteristics of the cells from the two sources would have different attachment capabilities and that laminin-332 may be in part responsible for this.…”

Section: Discussionmentioning

confidence: 99%

“…The pocket that is formed allows bacterial colonisation leading to an increased risk of infection. Translation of d e n t a l implant technology into a solution for attaching artificial limbs for amputees, could overcome the problems associated with traditional stump-socket devices, which include tissue necrosis, poor socket fitting, pain and functional impairment (Dudek et al, 2005;Lyon et al, 2000). However, despite common design concepts, skin-penetrating transcutaneous implants used for attachment of artificial limbs have been reported to have much higher infection rates compared with dental implants; with infectious complications being reported in 40 % of patients during the first 3 years post-operation (Tillander et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro

Pendegrass

Lancashire²,

Fontaine³

et al. 2015

eCM

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Infection is the primary failure modality for transcutaneous implants because the skin breach provides a route for pathogens to enter the body. Intraosseous transcutaneous amputation prostheses (ITAP) are being developed to overcome this problem by creating a seal at the skinimplant interface. Oral gingival epithelial cell attachment creates an infection-free seal around dental implants. However, this has yet to be achieved consistently outside of the oral environment. Epithelial cells attach to metal substrates by means of hemidesmosomes and focal adhesions. Their density per unit cell is an indicator of attachment strength. We postulate that gingival epithelial cells express more hemidesmosomes and focal adhesions at earlier time points, compared with epidermal keratinocytes, and this increased speed and strength of attachment may be the reason why an infection-free seal is often achieved around dental implants but less frequently around ITAP. The aim of this study was to compare epidermal keratinocyte with oral gingival cell attachment on titanium alloy in vitro, to determine whether these two cell types differ in their speed and strength of attachment. We aimed to test the hypothesis that gingival cells up-regulate focal adhesion and hemidesmosome formation at earlier time points compared with extra-oral keratinocytes. To test this hypothesis we cultured epidermal keratinocytes and oral gingival cells on titanium alloy substrates and assessed cell attachment by focal adhesions and hemidesmosome expression at 4, 24, 48 and 72 hours. Formation and expression of hemidesmosomes temporally lagged behind that of focal adhesions in both cell types. Gingival derived cells up-regulated focal adhesion and hemidesmosome expression at earlier time points compared with epidermal keratinocytes. Hemidesmosome expression in oral gingival cells was 3 times greater compared with epidermal keratinocytes at 4 hours. Our findings indicate that earlier attachment may be key to the success of the dental implant transcutaneous interface.

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“…We suggest that titanium that has been air-polished with tooth surface abrasives with large average particle diameter and high biocompatibility causes inflammation of the gingival epithelial cells and enhances the migration of white blood cells. Integrin 6 and integrin ß4 are involved in the wound healing of gingival epithelial tissue 25,26) , and are also associated with the adhesion and migration of junctional epithelium cells. Miyata et al 27) also reported that integrin 6 and integrin ß4 are involved in the epithelial boundary surface of the implant.…”

Section: Gene Expressionmentioning

confidence: 99%

Behavior of Human Gingival Epithelial Cells on Titanium Following Abrasion of the Adjunctive Glycine Air Polishing Powder

Koishi

Taguchi

Okuda

et al. 2016

J. Hard Tissue Biology.

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Treatment to replace missing teeth with implants has been available for nearly 50 years. However, many implants are subject to peri-implantitis because of resorption of the bone supporting the implant. Therefore periodontal maintenance to avoid bacterial infection around an implant device is essential. There are several methods of maintenance available to remove bacterial infections. One of these methods involves the direct removal of bacterial infection in the implant sulcus using tooth surface abrasives. Abrasives that contain glycine are recommended because of their biocompatibility with the tooth surface. The purpose of this study was to examine the cellular behavior of human gingival epithelial cells on the surface after abrasion with adjunctive glycine air-polishing powder. We treated the titanium surface with air-polishing abrasives containing glycine or sodium bicarbonate, then examined the structural surface change and wettability, as well as the proliferative potency and gene expression of adherent gingival epithelial cells. The glycine-containing abrasives exhibited higher biocompatibility than the sodium bicarbonate abrasive, and the use of abrasives with small average particle size may be useful in the removal of bacterial infections around implants.

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Involvement of laminin and integrins in adhesion and migration of junctional epithelium cells

Cited by 56 publications

References 27 publications

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro

Behavior of Human Gingival Epithelial Cells on Titanium Following Abrasion of the Adjunctive Glycine Air Polishing Powder

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