Involvement of lytic enzymes and secondary metabolites produced by Trichoderma spp. in the biological control of Pythium myriotylum

Tchameni, Séverin Nguemezi; Cotârleț, Mihaela; Ghinea, Ioana Otilia; Bedine, Marie Amperes Boat; Sameza, Modeste Lambert; Borda, Daniela; Bahrim, Gabriela; Dinică, Rodica Mihaela

doi:10.1007/s10123-019-00089-x

Cited by 35 publications

(13 citation statements)

References 29 publications

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“…In this study, whole cells of COW3 showed in vitro antagonistic activity against P. myriotylum CMR1 with clear zones of inhibition after two days, coupled with mycophagy after four days of co-incubation with the pathogen. A recent study reports that Trichoderma strains with in vitro antagonism against P. myriotylum all produced cellulases, proteases, and xylanases [42]. It remains to be investigated whether this is also the case for our bacterial strain Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

et al. 2019

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Pseudomonas species are metabolically robust, with capacity to produce secondary metabolites including cyclic lipopeptides (CLPs). Herein we conducted a chemical analysis of a crude CLP extract from the cocoyam rhizosphere-derived biocontrol strain Pseudomonas sp. COW3. We performed in silico analyses on its whole genome, and conducted in vitro antagonistic assay using the strain and purified CLPs. Via LC-MS and NMR, we elucidated the structures of four novel members of the bananamide group, named bananamides D-G. Besides variability in fatty acid length, bananamides D-G differ from previously described bananamides A-C and MD-0066 by the presence of a serine and aspartic acid at position 6 and 2, respectively. In addition, bananamide G has valine instead of isoleucine at position 8. Kendrick mass defect (KMD) allowed the assignment of molecular formulae to bananamides D and E. We unraveled a non-ribosomal peptide synthetase cluster banA, banB and banC which encodes the novel bananamide derivatives. Furthermore, COW3 displayed antagonistic activity and mycophagy against Pythium myriotylum, while it mainly showed mycophagy on Pyricularia oryzae. Purified bananamides D-G inhibited the growth of P. myriotylum and P. oryzae and caused hyphal distortion. Our study shows the complementarity of chemical analyses and genome mining in the discovery and elucidation of novel CLPs. In addition, structurally diverse bananamides differ in their antimicrobial activity.

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Section: Discussionmentioning

confidence: 99%

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

et al. 2019

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“…Tr ichoderma sp. also produce metabolites with antimicrobial activity and different enzymes such as chitinases, glucanases, proteases and pectinases that degrade the cell wall of pathogenic fungi (Verma et al 2007; Tchameni et al 2019). Besides antibiosis and enhanced host–plant resistance, they also deploy other antagonist mechanisms such as parasitism and competition (Benítez et al 2004; Schuster and Schmoll 2010; Guzmán‐Guzmán et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata , Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges ( Citrus sinensis L. (Osbeck))

et al. 2020

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Aims: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporioides are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. Methods and Results: Seven strains were isolated and identified as Trichoderma harzianum, T. guizhouense, T. atroviride and T. koningiopsis through morphological and molecular analyses. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC-30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80% after 3 weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activities. Conclusions: Trichoderma harzianum IC-30 is an optimal antagonist for the control of green mould spreading and other pathogens in post-harvest citrus fruits. Significance and Impact of the Study: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides.

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“…Screening of antifungal activity was carried out using the dual culture method on PDA (Tchameni et al 2020). Test bacteria were streaked in a straight line at the center of a plate.…”

Section: Isolation Identi Cation and Antifungal Screeningmentioning

confidence: 99%

Antifungal compound from marine Serratia marcescens BKACT and its potential activity against Fusarium sp.

2022

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Ecofriendly biocontrol agents to control of pathogenic fungi are in demand globally. The present study was evaluated the antifungal potentials of marine bacteria Serratia marcescens BKACT against eight different Fusarium species. A highest 75.5 ± 0.80% of mycelial inhibition was observed against Fusarium foetens NCIM 1330. Structural characterization of the puri ed compound was analyzed by GC-MS and NMR techniques, based on the analysis it is con rmed as 2, 4-di-tert butyl phenol (2, 4-DTBP) with chemical structure C 14 H 22 O. at 0.53mM concentration puri ed compound inhibited complete spore germination of F. foetens NCIM 1330. In vitro assay showed complete inhibition of F. foetens NCIM 1330 on the wheat seeds. Tested concentration doesn't show any toxic effect on germination of the seeds. By this study we conclude that, 2, 4-DTBP a suitable candidate to be used as biocontrol agent against Fusarium infection.

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Involvement of lytic enzymes and secondary metabolites produced by Trichoderma spp. in the biological control of Pythium myriotylum

Cited by 35 publications

References 29 publications

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata , Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges ( Citrus sinensis L. (Osbeck))

Antifungal compound from marine Serratia marcescens BKACT and its potential activity against Fusarium sp.

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