Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

Jeffroy, F.; Paillard, Christine

doi:10.1016/j.fsi.2011.10.008

Cited by 23 publications

(13 citation statements)

References 36 publications

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“…This coincided with an increase of granulocytes associated with an increase in phagocytosis (Allam et al, 2001). Recently, it has been demonstrated that levels of nitric oxide in the hemolymph increased in proportion with quantities of circulating V. tapetis (Jeffroy and Paillard, 2011).…”

Section: Tapetismentioning

confidence: 85%

Bacterial diseases in marine bivalves

Travers

Miller

Roque

et al. 2015

Journal of Invertebrate Pathology

146

121

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Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.

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Section: Tapetismentioning

confidence: 85%

Bacterial diseases in marine bivalves

Travers

Miller

Roque

et al. 2015

Journal of Invertebrate Pathology

146

121

View full text Add to dashboard Cite

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“…In marine invertebrates, significant increase of NO/nitrite has contributed on the innate immunity as shown in T. japonicus. NO production in hemolymph and extrapallial fluid of the Manila clam Ruditapes philippinarum was dose dependent and was increased in response to Vibrio tapetis challenge (Jeffroy and Paillard, 2011). The total activity of NOS and NO concentration in the haemolymph of scallop C. farreri was increased significantly after LPS stimulation (Jiang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Identification and molecular characterization of nitric oxide synthase (NOS) gene in the intertidal copepod Tigriopus japonicus

Jeong

Kang

Seo

et al. 2016

Gene

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“…The results revealed that the expression of CfNOS mRNA could be quickly triggered by different immune stimuli, suggesting the inducible activity of CfNOS during scallop immune defense and the involvement of CfNOS in a broad-spectrum of immune responses against the fungi, Gram-negative and -positive bacteria. Mollusc NO has been reported to be involved in the immune defense [46–48], and the iNOS-like activity was detected after the stimulation of LPS in the haemocytes of scallop C . farreri [49].…”

Section: Discussionmentioning

confidence: 99%

A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense

et al. 2013

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BackgroundNitric oxide synthase (NOS) is responsible for synthesizing nitric oxide (NO) from L-arginine, and involved in multiple physiological functions. However, its immunological role in mollusc was seldom reported.MethodologyIn the present study, an NOS (CfNOS) gene was identified from the scallop Chlamys farreri encoding a polypeptide of 1486 amino acids. Its amino acid sequence shared 50.0~54.7, 40.7~47.0 and 42.5~44.5% similarities with vertebrate neuronal (n), endothelial (e) and inducible (i) NOSs, respectively. CfNOS contained PDZ, oxygenase and reductase domains, which resembled those in nNOS. The CfNOS mRNA transcripts expressed in all embryos and larvae after the 2-cell embryo stage, and were detectable in all tested tissues with the highest level in the gonad, and with the immune tissues hepatopancreas and haemocytes included. Moreover, the immunoreactive area of CfNOS distributed over the haemocyte cytoplasm and cell membrane. After LPS, β-glucan and PGN stimulation, the expression level of CfNOS mRNA in haemocytes increased significantly at 3 h (4.0-, 4.8- and 2.7-fold, respectively, P < 0.01), and reached the peak at 12 h (15.3- and 27.6-fold for LPS and β-glucan respectively, P < 0.01) and 24 h (17.3-fold for PGN, P < 0.01). In addition, TNF-α also induced the expression of CfNOS, which started to increase at 1 h (5.2-fold, P < 0.05) and peaked at 6 h (19.9-fold, P < 0.01). The catalytic activity of the native CfNOS protein was 30.3 ± 0.3 U mgprot-1, and it decreased significantly after the addition of the selective inhibitors of nNOS and iNOS (26.9 ± 0.4 and 29.3 ± 0.1 U mgprot-1, respectively, P < 0.01).ConclusionsThese results suggested that CfNOS, with identical structure with nNOS and similar enzymatic characteristics to nNOS and iNOS, played the immunological role of iNOS to be involved in the scallop immune defense against PAMPs and TNF-α.

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Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

Cited by 23 publications

References 36 publications

Bacterial diseases in marine bivalves

Bacterial diseases in marine bivalves

Identification and molecular characterization of nitric oxide synthase (NOS) gene in the intertidal copepod Tigriopus japonicus

A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense

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