Involvement of oligosaccharides and sucrose-related genes on sucrose retention in strawberries from ripening to shelf-life

Olmo, Iván del; Bałanda, Maria; Romero, Irene; Vázquez-Hernández, María; Sanchez-Ballesta, Marı́a T.; Escribano, M. Isabel; Merodio, Carmen

doi:10.1016/j.postharvbio.2020.111301

Cited by 10 publications

(10 citation statements)

References 56 publications

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“…Also, there is a decrease in the expression of FvH4_2g12370, encoding an α-galactosidase 1. The present results follow the quantitative determination of raffinose family of oligosaccharides (RFOs; raffinose and stachyose) in strawberries (del Olmo et al, 2020), indicating a sustained synthesis of RFOs in stressed air-cold stored strawberries. Accumulation of trisaccharide raffinose has been also described as a cold-inducible biosynthetic route in other fruit and vegetables (Bustamante et al, 2016;Blanch et al, 2017) and different functional roles have been suggested for RFOs (Elsayed et al, 2014).…”

Section: Discussionsupporting

confidence: 83%

“…GDP-mannose and GDP-galactose are also involved in the first steps of the ascorbic acid biosynthesis (Badejo et al, 2008). The metabolism of activated sugars is closely related to sucrose catabolism, which we already reported as being controlled by high CO 2 treatment (del Olmo et al, 2020). UDP-glucose can be converted to other activated sugars and it is also an entrance step to the synthesis of RFOs.…”

Section: Discussionmentioning

confidence: 93%

“…For the contribution to osmotic adjustment for turgor maintenance and cell wall structure, the metabolism of sugars, especially sucrose, the main reserve carbohydrate in strawberries, takes on particular importance. We previously reported that in the diploid Mara des Bois strawberries, other beneficial responses were found concerning high CO 2 treatment, primarily sucrose accumulation and water retention (del Olmo et al, 2020).…”

Section: Introductionmentioning

confidence: 95%

“…A calibration curve of H 2 O 2 (Sigma-Aldrich) from 0 to 150 μM was prepared (y = 0.0011x-0.0015; R 2 = 0.9997). For sugar analyses, 2 g of frozen strawberry powder (wet) were extracted with 5 ml of distilled water and soluble glucose, fructose, sucrose and xylose were determined as described by del Olmo et al (2020). Experimental data represent the mean and SD of the three replicates.…”

Section: Glutathione Lignin H 2 O 2 and Major Soluble Sugarsmentioning

confidence: 99%

“…We selected the hexoses/sucrose ratio as indicator of senescence-like process During stress and in senescing leaves, hexose sugars often accumulate, resulting in an increased hexose/ sucrose ratio (Wingler and Roitsch, 2008). In addition, we previously reported that CO 2 -treated strawberries reduced sucrose degradation primarily linked to a down-regulation of vacuolar invertase (FvVINV2) and cell wall invertase (FvCWINV1) transcripts (del Olmo et al, 2020). Although little is known about senescence-specific marker in fruit, we suggest that the relative ratio between hexoses and sucrose rather than the absolute concentration of sugars may be a good marker of senescence-like process in fruit.…”

Section: Strawberries With Contrasting Cold Storage Exhibiting Differ...mentioning

confidence: 99%

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Transcriptomic analysis of CO2-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption

Olmo

Romero²,

Álvarez³

et al. 2022

Front. Plant Sci.

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One of the greatest threats to wild strawberries (Fragaria vesca Mara des Bois) after harvest is the highly perishability at ambient temperature. Breeders have successfully met the quality demands of consumers, but the prevention of waste after harvest in fleshy fruits is still pending. Most of the waste is due to the accelerated progress of senescence-like process after harvest linked to a rapid loss of water and firmness at ambient temperature. The storage life of strawberries increases at low temperature, but their quality is limited by the loss of cell structure. The application of high CO2 concentrations increased firmness during cold storage. However, the key genes related to resistance to softening and cell wall disassembly following transference from cold storage at 20°C remain unclear. Therefore, we performed RNA-seq analysis, constructing a weighted gene co-expression network analysis (WGCNA) to identify which molecular determinants play a role in cell wall integrity, using strawberries with contrasting storage conditions, CO2-cold stored (CCS), air-cold stored (ACS), non-cold stored (NCS) kept at ambient temperature, and intact fruit at harvest (AH). The hub genes associated with the cell wall structural architecture of firmer CO2-treated strawberries revealed xyloglucans stabilization attributed mainly to a down-regulation of Csl E1, XTH 15, Exp-like B1 and the maintenance of expression levels of nucleotide sugars transferases such as GMP and FUT as well as improved lamella integrity linked to a down-regulation of RG-lyase, PL-like and PME. The preservation of cell wall elasticity together with the up-regulation of LEA, EXPA4, and MATE, required to maintain cell turgor, is the mechanisms controlled by high CO2. In stressed air-cold stored strawberries, in addition to an acute softening, there is a preferential transcript accumulation of genes involved in lignin and raffinose pathways. Non-cold stored strawberries kept at 20°C after harvest are characterized by an enrichment in genes mainly involved in oxidative stress and up-expression of genes involved in jasmonate biosynthesis. The present results on transcriptomic analysis of CO2-treated strawberries with enhanced resistance to softening and oxidative stress at consumption will help to improve breeding strategies of both wild and cultivated strawberries.

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