Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in

Drakulic, T; Temple, Mark D.; Guido, Ron; Jarolim, Stefanie; Breitenbach, Michael; Attfield, Paul V.; Dawes, Ian W.

doi:10.1016/j.femsyr.2005.06.001

Cited by 127 publications

(98 citation statements)

References 69 publications

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“…The glyoxalase pathway in the fermenting yeast mainly disposes of methylglyoxal, formed as a by-product of glycolysis from triose phosphates, and is dependent on glutathione [24]. Another important parameter, not evaluated in this study, is the redox potential of cellular glutathione, dependent on the concentration of both the reduced and oxidized forms of this compound [25,26]. The value of this parameter may also be dependent on the source of carbon in the yeast cultures.…”

Section: Resultsmentioning

confidence: 95%

The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Macierzyńska

Grzelak

Bartosz

2007

Cellular and Molecular Biology Letters

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Abbreviations used: DTNB -5,5'-dithiobis-(2-nitrobenzoic acid); H 2 R 123 -dihydrorhodamine 123; MES -2-morpholinoethanesulfonic acid; ROS -reactive oxygen species; SOD -superoxide dismutase; YPD medium -1% yeast extract, 1% Bacto-peptone, 2% glucose, YPG medium, 1% yeast extract, 1% Bacto-peptone,2% glycerol; YPE medium -1% yeast extract, 1% Bacto-peptone, 3% ethanol Abstract: We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.

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Section: Resultsmentioning

confidence: 95%

The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Macierzyńska

Grzelak

Bartosz

2007

Cellular and Molecular Biology Letters

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“…Although yeast cells, whether grown in SCD, CR or in SCG, did not show major differences in exponential phase, CR and SCG greatly extended the lifespan of chronologically aged cells as compared to SCD. When respiration was the only way to produce ATP, more than 40 % of the cells were still viable after 10 days; this might be related to the low production of ROS [36,37]. In fact an increase in the ROS production could clearly be observed in senescent cells grown in standard glucose concentration.…”

Section: Discussionmentioning

confidence: 99%

Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing

Magherini

Carpentieri

Amoresano

et al. 2009

Cell. Mol. Life Sci.

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Abstract. In this study, a proteomic approach that combines selective labelling of proteins containing reduced cysteine residues with two-dimensional electrophoresis/mass spectrometry was used to evaluate the redox state of protein cysteines during chronological ageing in Saccharomyces cerevisiae. The procedure was developed on the grounds that biotinconjugated iodoacetamide (BIAM) specifically reacts with reduced cysteine residues. BIAM-labelled proteins can then be selectively isolated by streptavidin affinity capture. We compared cells grown on 2 % glucose in the exponential phase and during chronological ageing and we found that many proteins undergo cysteine oxidation. The target proteins include enzymes involved in glucose metabolism. Both caloric restriction and growth on glycerol resulted in a decrease in the oxidative modification. Furthermore, in these conditions a reduced production of ROS and a more negative glutathione half cell redox potential were observed.

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“…2B, panel 5), a protein central for sulfur metabolism and glutathione synthesis (37). Glutathione is a known antioxidant in yeast (13,38). Accumulation of this protein in mitochondrial extracts hints to an increased oxidative stress in cdc48 S565G cells (see below).…”

Section: Identified Proteins Differentially Found In Mitochondrial Exmentioning

confidence: 99%

Crucial Mitochondrial Impairment upon CDC48 Mutation in Apoptotic Yeast

Braun¹,

Zischka

Madeo

et al. 2006

Journal of Biological Chemistry

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Mutation in CDC48 (cdc48 S565G ), a gene essential in the endoplasmic reticulum (ER)-associated protein degradation (ERAD) pathway, led to the discovery of apoptosis as a mechanism of cell death in the unicellular organism Saccharomyces cerevisiae. Elucidating Cdc48p-mediated apoptosis in yeast is of particular interest, because Cdc48p is the highly conserved yeast orthologue of human valosin-containing protein (VCP), a pathological effector for polyglutamine disorders and myopathies. Here we show distinct proteomic alterations in mitochondria in the cdc48 S565G yeast strain. These observed molecular alterations can be related to functional impairment of these organelles as suggested by respiratory deficiency of cdc48 S565G cells. Mitochondrial dysfunction in the cdc48 S565G strain is accompanied by structural damage of mitochondria indicated by the accumulation of cytochrome c in the cytosol and mitochondrial enlargement. We demonstrate accumulation of reactive oxygen species produced predominantly by the cytochrome bc 1 complex of the mitochondrial respiratory chain as suggested by the use of inhibitors of this complex. Concomitantly, emergence of caspase-like enzymatic activity occurs suggesting a role for caspases in the cell death process. These data strongly point for the first time to a mitochondrial involvement in Cdc48p/VCPdependent apoptosis.Fundamental cellular processes, such as the formation of organelles (ER,3 Golgi apparatus, and the nuclear envelope), or ubiquitin-dependent ER-associated protein degradation (ERAD) have been linked to the yeast protein Cdc48p and its highly conserved mammalian orthologue VCP (1-4). Mutations in VCP have been associated with "inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia" (IBMPFD), a dominant human disorder (5, 6). A genetic screening of a Drosophila model for human polyglutamine diseases, a class of inherited neurodegenerative disorders, identified the Drosophila homologue of Cdc48p/VCP as a modulator of apoptotic cell death (7), leading these authors to propose VCP as a pathological effector for polyglutamine-induced neurodegeneration. However, the cellular mechanisms underlying VCP-mediated cell death in these human disorders remain largely unknown. Apoptotic phenotypes in cells expressing mutated Cdc48p/ VCP have originally been described in budding yeast (8) and were thereafter confirmed in mammalian cell cultures (9, 10), in trypanosomes (11), and in zebrafish (12). Notably, Cdc48p was the first apoptotic mediator found in Saccharomyces cerevisiae (8). The expression of a point-mutated CDC48 gene (cdc48 S565G ) leads to a characteristic apoptotic phenotype: phosphatidylserine externalization, DNA fragmentation, chromatin condensation, nuclear fragmentation, and vacuolization (8, 13). These results obtained in the cdc48 S565G strain initiated the establishment of yeast as a model to study evolutionary conserved mechanisms of apoptotic regulation (14 -16).Mitochondria play a crucial role in many apoptotic pathways in ...

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Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in

Cited by 127 publications

References 69 publications

The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing

Crucial Mitochondrial Impairment upon CDC48 Mutation in Apoptotic Yeast

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