Involvement of peroxidases in structural changes of barley stem

Begović, Lidija; Lepeduš, Hrvoje; Lalić, Alojzije; Štolfa, Ivna; Jurković, Zorica; Kovačević, Josip; Cesar, Vera

doi:10.1590/1678-4499.155

Cited by 6 publications

(7 citation statements)

References 29 publications

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“…It was shown, that in barley stem in mature internode cationic isoforms of peroxidases were active, while in young internode-anionic isoforms [19]. Our data confirm this: we have found the high BPOX activity at the early stages of zinnia development, and GPOX was active in mature tissues.…”

Section: Discussionsupporting

confidence: 90%

“…Thus, at the stage of intensive elongation the lignin content was lower than in old stem tissues. It was shown that anionic as well as cationic peroxidases are involved in the lignification, but they use different phenolics as substrates [4,5,19]. H2O2 is also the substrate for peroxidases, involved in lignin synthesis, and its content increased in internode tissues for 40 days by 33.2%, and then did not change up to 60 days (see Table 1).…”

Section: Resultsmentioning

confidence: 99%

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Role of Class III Peroxidases in Stem Lignification of Zinnia elegans Jacq.

Tugbaeva

Ermoshin

Plotnikov

et al. 2020

The 1st International Electronic Conference on Plant Science

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Class III peroxidases (EC 1.11.1.7) have use a wide range of substrates and perform numerous functions, including synthesis of monolignols, lignin precursors. The activity and tissue localization of cationic guaiacol (GPOX) and anionic benzidine peroxidases (BPOX) were studied in the first internode of zinnia plants of different ages. The lignin in cross sections of plant tissues was stained by phloroglucinol-HClTthe lignin content was determined by Klason. Enzyme activity and the H2O2 amount were determined spectrophotometrically. The hypocotyl grew for 40 days and the lignin content for 60 days. In 20-days-old plants, lignin was detected in protoxylem, and in 60-days-old—in sclerenchyma, protoxylem, and metaxylem. Enzyme histochemistry revealed that BPOX was localized in endoderm, phloem, and protoxylem, while GPOX—in the metaxylem and sclerenchyma. A moderate increase in GPOX activity during internode growth was shown. In contrast, BPOX activity was high at the initial growth stage, and declined to the 60th day. Thus, the most intense lignification in mechanical tissue and xylem occurred during the period from 20 to 40 days of plant growth. BPOX is likely involved in the process at the early stages of growth, while GPOX is responsible for sclerenchyma and metaxylem lignification at the later stages.

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Section: Discussionsupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Role of Class III Peroxidases in Stem Lignification of Zinnia elegans Jacq.

Tugbaeva

Ermoshin

Plotnikov

et al. 2020

The 1st International Electronic Conference on Plant Science

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“…Peroxidase is present in almost all living organisms. In plants, they participate in myriad biological processes, such as stress response, lignification, and most importantly, the defense against pathogens. ,, This oxidoreductase enzyme performs a protective activity due to its capability to catalyze the oxidative cross-linking of proteins and phenolics in the plant cell wall, protecting the plant from degradation. , The peroxidase superfamily comprises three classes in which class III contains the most representative barley grain peroxidase group, BP1. The structure of the peroxidase BP1 (Figure c) was determined by Henriksen et al, revealing a heme group sandwiched between a distal N-terminal domain and a proximal C-terminal domain.…”

Section: Resultsmentioning

confidence: 99%

“…In plants, they participate in myriad biological processes, such as stress response, lignification, and most importantly, the defense against pathogens. 59,76,77 This oxidoreductase enzyme performs a protective activity due to its capability to catalyze the oxidative cross-linking of proteins and phenolics in the plant cell wall, protecting the plant from degradation. 59,78 The peroxidase superfamily comprises three classes in which class III contains the most representative barley grain peroxidase group, BP1.…”

Section: Effect Of Hop Bitter Acids On Wort Composition Andmentioning

confidence: 99%

Addition of Hop (Humulus Lupulus L.) Bitter Acids Yields Modification of Malt Protein Aggregate Profiles during Wort Boiling

Pinto

Schmidt

Rappsilber

et al. 2023

J. Agric. Food Chem.

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Hop bitter acids are used in the brewing industry to give beer bitterness. However, much of this bitterness is lost during processing, specifically during the wort boiling step. One of the major causes might be the interaction with protein–protein complexes. Therefore, the aim of this study was to clarify the role of hop bitter acids in protein aggregate formation using a proteomic approach. The effect of hop addition on protein composition was analyzed by liquid chromatography–mass spectrometry/MS (LC-MS/MS), and further analyses were performed to characterize the wort before and after boiling. Addition of hop bitter acids yielded a change in wort protein profiles, and hop bitter acids were found to bind primarily to less abundant proteins which are not related to beer quality traits, such as foam or haze. Wort protein aggregate profiles were revealed, and findings from this study suggested the precipitation of particular proteins in the aggregates during boiling when hops were added.

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“…Phenols can act in different ways to prevent the development of diseases. Phytoalexins have fungicidal activity 39 while monolignols are substrates for GPX enzymes during the synthesis of lignin 40 . Lignin deposition leads to the thickening of the cell wall and prevents the penetration of the disease agents into the cells 41 .…”

Section: Discussionmentioning

confidence: 99%

Paraffin oil induces resistance against powdery mildew in grapevine through salicylic acid signaling

Pálfi

Lovas

Zsófi³

et al. 2021

Pest Management Science

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BACKGROUND Mineral oils have been widely used in the pest control of several crops. However, their mode of action is poorly understood, especially in the case of their antifungal properties. The possible direct fungicidal activity and the stress‐inducing capability of paraffin oil on grapevine were examined using Vitis vinifera L. cv ‘Kékfrankos’ cuttings and the fungus Erysiphe necator, the causal agent of powdery mildew. RESULTS Our experiments demonstrated that paraffin oil does not have fungicide activity on E. necator, but induces significant stress‐related changes in grapevine physiology. Elevated H2O2 production and the accumulation of the phytohormone salicylic acid were measured. Secondary thickening of the cell wall by lignin deposition and the accumulation of phenolic compounds were also observed. Some enzyme activities related to the detoxification of reactive oxygen species, disease response, and the synthesis of lignin were changed in accordance with the determined changes in cell wall composition and H2O2 levels. CONCLUSION The results suggest that paraffin oil induces stress responses on grapevine leaves through oxidative burst, and this response is systemized by salicylic acid. The accumulation of lignin and water‐soluble phenolics may be directly responsible for the paraffin oil‐induced resistance of grapevine against powdery mildew. © 2021 Society of Chemical Industry.

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Involvement of peroxidases in structural changes of barley stem

Cited by 6 publications

References 29 publications

Role of Class III Peroxidases in Stem Lignification of Zinnia elegans Jacq.

Role of Class III Peroxidases in Stem Lignification of Zinnia elegans Jacq.

Addition of Hop (Humulus Lupulus L.) Bitter Acids Yields Modification of Malt Protein Aggregate Profiles during Wort Boiling

Paraffin oil induces resistance against powdery mildew in grapevine through salicylic acid signaling

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