Involvement of staphylococcal protein A and cytoskeletal actin in Staphylococcus aureus invasion of cultured human oral epithelial cells

Abstract

Following the coincidental discovery that â-actin isolated from renal epithelial cells was precipitated by staphylococcal protein A (SPA), the possibility that SPA and cytoskeletal actin ®laments may be involved in Staphylococcus aureus infection of epithelial cells was considered. Therefore, to clarify the potential role of SPA and actin ®laments in S. aureus infection, the invasion ef®ciency of S. aureus was determined quantitatively by measuring the number of cfu of viable organisms recovered from cultured KB cells. S. aureus invasion was found to be time dependent (0±60 min) and increased linearly when increasing numbers of bacteria were added (10 4 ±10 6 cfu/ml). However, signi®cant variation in the level of invasion was noted in protein A-de®cient S. aureus Wood 46. Cytochalasin B inhibited the invasion ef®ciency of S. aureus in a dose-dependent manner. The present study suggests that interaction of staphylococcal protein A and cytoskeletal actin ®laments is involved in the S. aureus invasion of cultured KB cells, and this process may contribute, in part, to the intracellular movement, cell-to-cell spread and dissemination of S. aureus within human oral epithelial cells in vivo.

Antibacterial activity of Curcuma longa L. against methicillin‐resistant Staphylococcus aureus

“…Surprisingly, C. longa extract inhibited the MRSA invasion of HMFs. In the invasion mechanism of Staphylococcus aureus, Staphylococcal protein A (SPA) may have an important role (Jung et al, 2001). Additional experiments are required to determine the possibility of inhibition of SPA role by C. longa extract.…”

“…Bacterial invasion into cells and tissues is one of the important pathogenic mechanisms in oral infection (Schuster and Burnett, 1981). To investigate the inhibitory effect of an ethyl acetate extract of C. longa, which exhibited the highest antimicrobial activity on bacterial invasion to cultured monolayers of HMFs, previously reported methods were used with a slight modification (Jung et al, 2001). HMFs were obtained from the patients undergoing oral surgery.…”

Internalization of Staphylococcus aureus by Human Keratinocytes

“…Indeed, the internalization of S. aureus by endothelial cells (25), osteoblasts (1; Nair, unpublished data), and 293 cells (41) has been shown to require the host cell integrin ␣5␤1 and the S. aureus fibronectin-binding MSCRAMM FnBPA and/or FnBPB. However, while there is little dispute that the S. aureus FnBPs are involved in bacterial uptake, studies with a variety of epithelial cells have provided conflicting evidence for an essential role for the FnBPs in the internalization process (8,14,19,21). The differences may be due to the various sources of the epithelial cells and their nature (for example, immortalized cells versus normal primary cells).…”

Inhibitory effects of Caesalpinia sappan on growth and invasion of methicillin-resistant Staphylococcus aureus

“…To investigate the inhibitory effect of methanol extract of Caesalpinia sappan, which exhibited the highest antimicrobial activity, on bacterial invasion to cultured monolayers of HMFs, previously reported methods were used with a slight modification (Jung et al, 2001). HMFs were obtained from the patients undergoing oral surgery.…”