Involvement of the Chox-4 chicken homeobox genes in determination of anteroposterior axial polarity during limb development

Nohno, Tsutomu; Noji, Sumihare; Koyama, Eiki; Ohyama, Kazumi; Myokai, Fumio; Kuroiwa, Atsushi; Saito, Taiichi; Taniguchi, Shigehiko

doi:10.1016/0092-8674(91)90274-3

Cited by 210 publications

(67 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously reported (Dolle et al, 1989;Izpisua-Belmonte et al, 1991;Nohno et al, 19911, we have found that at early stages of limb development GHox-4.6 transcripts are present in high amounts throughout the posterior half of the limb mesoderm and are absent from the anterior half of the limb mesoderm. This expression pattern is consistent with the studies implicating GHox-4.6 in the specification of posterior positional identity (IzpisuaBelmonte et al, 1991;Nohno et al, 1991) and is complementary to the expression pattern of GHox-8 and GHox-7 which have been suggested to be involved in specification of anterior positional identity (Coelho et al, 1991b;Coelho et al, submitted).…”

Section: Discussionsupporting

confidence: 89%

“…At early stages of limb development when positional identities along the anteriorposterior axis are being specified, several HOX genes exhibit graded complementary and overlapping domains of expression across the anterior-posterior axis. At these stages, the chicken HOX genes GHox-8 (Coelho et al, 1991b), submitted), and GHox-2.1 (Wedden et al, 1989) are expressed predominantly in the anterior mesoderm of the limb bud, whereas several members of the HOX-4 cluster including are expressed in the posterior mesoderm of chick and mouse limb buds (Dolle et al, 1989;Izpisua-Belmonte et al, 1991;Nohno et al, 1991). These observations suggest that interacting opposing gradients of expression of various HOX genes may be involved in specifying positional values.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The pattern of expression of the chicken homolog of HOX1I in the developing limb suggests a possible role in the ectodermal inhibition of chondrogenesis

Rogina

Coelho

Kosher

et al. 1992

Developmental Dynamics

View full text Add to dashboard Cite

Homeobox-containing genes have been implicated in a variety of patterning events during vertebrate limb development. In an attempt to isolate cDNAs corresponding to 5' members of the chicken HOX 4 cluster of homeobox-containing genes, a cDNA library constructed from mRNAs expressed during early stages of chick limb development was screened with probes generated by the polymerase chain reaction (PCR) using oligonucleotide primers corresponding to sequences in the homeoboxes of the human HOX4C and HOX4F genes, the human homologs of Hox-4.4 and Hox-4.6. This screening resulted in the isolation of full length cDNAs for the chicken homolog of HOX4F (cognate of mouse Hox-4.6), which we have termed GHox-4.6, and the chicken homolog of human HOXlI, which we have named GHox-li, a paralog of Hox-4.6 in the HOX 1 cluster. The homeodomains encoded by GHox-4.6 and GHox-li differ by only three amino acids, and the two proteins show extensive similarity along their entire lengths. Despite their sequence similarity, in situ hybridization analysis has revealed that GHox-4.6 and GHox-li exhibit strikingly different spatial patterns of expression during embryonic chick limb development. At early stages of limb development (stages 20-22), GHox-4.6 transcripts are present in high amounts throughout the posterior half of the limb mesoderm and are absent from the anterior half of the mesoderm, an expression pattern consistent with the possible involvement of GHox-4.6 in the specification of posterior positional identity. In contrast, GHox-li exhibits no distinct anterior-posterior polarity of expression at stage 22, but rather is expressed in high amounts throughout the mesenchyme of the limb bud. At later stages of development (stage 25), GHox-li continues to be expressed in high amounts throughout the undifferentiated mesenchyme subjacent to the apical ectodermal ridge, and, in addition, is expressed in the mesodermal cells in the proximal peripheral regions of the limb bud subjacent to the ectoderm which are differentiating into nonchondrogenic lineages. bud where chondrogenic differentiation is occurring. Thus, GHox-li is expressed by the undifferentiated subridge mesenchymal cells and proximal peripheral mesenchymal cells of the limb bud that are being inhibited from undergoing chondrogenesis by the apical ectodermal ridge and nonridge ectoderm. These observations suggest the possibility that GHox-li may be involved in mediating the antichondrogenic effect of limb ectoderm, and that the cessation of GHox-li expression may be required for chondrogenic differentiation to occur in the proximal central core of the limb bud.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

The pattern of expression of the chicken homolog of HOX1I in the developing limb suggests a possible role in the ectodermal inhibition of chondrogenesis

Rogina

Coelho

Kosher

et al. 1992

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…In closing, we may ask what mechanisms may bring about the complex patterns of syndecan and tenascin gene expression described above. One obvious possibility to consider is the expression patterns of regulatory genes, such as those belonging to the H o d and H o d clusters, in the developing limb (Dolle et al, 1989;Nohno et al, 1991;Yokouchi et al, 1991;Haack and Gruss, 1993). For example, at stage 24-26 the distal boundary of Hoxa-10 gene expression occurs a t the branching point of the Y-shaped condensation while the anterior boundary of Hoxd-11 and Hoxd-12 appears to occur between the future radius and ulna.…”

Section: Discussionmentioning

confidence: 99%

Syndecan‐3, tenascin‐C, and the development of cartilaginous skeletal elements and joints in chick limbs

Koyama

Leatherman

Shimazu

et al. 1995

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

The mechanisms by which the early limb cell Condensations and interzone mesenchyme give rise to skeletal elements and joints are poorly understood. Previous work from this laboratory has shown that the extracellular matrix protein tenascin-C is associated with articular cartilage and joint tissue development; others have shown that tenascin-C may exert its biological activities via interactions with cell surface receptors, such as syndecans. To further analyze the roles of tenascin-C and its putative receptors in skeletal development, we carried out a detailed in situ hybridization analysis of tenascin-C and syndecan-3 gene expression during development of chick limb skeletal elements and joints. We found that as the early mesenchymal condensations chondrify around day 5 (E5) of development, they become surrounded by a thick syndecan-3 rich perichondrium while tenascin-C transcripts are much fewer and restricted to diaphyseal perichondrium and developing interzones. Similar patterns were observed as distal carpal and digit condensations formed in older embryos. As the cartilaginous long bone models elongated proximo-distally and joint formation proceeded with age, we observed that syndecan-3 transcripts decrease significantly along the diaphysis and remain very abundant along the metaphysis and in the epiphyseal articular cap and interzone. Conversely, tenascin-C RNAs remain abundant along the diaphysis and begin to increase at the epiphysis and in interzone-derived tissues, such as menisci and joint capsule. By E10, the skeletal elements have well-defined morphologies, endochondral ossification has initiated in their diaphysis, and diaphyseal perichondrium has become periosteum. These developmental changes were accompanied by equally marked changes in gene expression; these included a marked increase in tenascin-C gene expression in articular cap, fragmentation of tenascin-C gene expression along the periosteum, reinitiation of syndecan-3 gene expression in periosteum, and differential gene expression in osteoprogenitor cells. The sheer complexity of the gene expression patterns documented in this study attests to the complexity of processes that bring about normal skelatogenesis. Clearly,tenascin-C and syndecan-3 appear to be closely associated with several of these processes, particularly in establishing tissue boundaries (perichondrium and periosteum) between condensations and surrounding mesenchymal cells, in regulating perichondral cell differentiation and incorporation into the growing skeletal elements, and in the genesis of epiphyseal chondrocytes and associated joint tissues. o 1995 Wiley-Liss, Inc.

show abstract

“…A similar pattern is found in limb buds where again the spatial and temporal order of expression reflects the organisation of the corresponding cluster (Dollk et al, 1989;Izpisua-Belmonte et al, 1991 ;Nohno et al, 1991). Whereas Drosophila homeo-boxcontaining genes have been shown to encode transcriptional regulators, such function still has to be demonstrated for their mouse counterparts.…”

Section: Transcriptional Regulatorsmentioning

confidence: 66%

The molecular and genetic analysis of mouse development

Gossler¹,

Balling

1992

European Journal of Biochemistry

View full text Add to dashboard Cite

This review describes some recent advances in the molecular‐genetic analysis of mouse development. Reversed genetics and gene assignment have been used to isolate genes affected in developmental mutations. The establishment of a high‐density molecular‐genetic map promises to facilitate cloning of additional genes with developmental functions. Based on molecular, biochemical or other biological criteria many mouse genes that code for transcriptional regulators, growth‐factor‐like molecules and their receptors have been isolated. The role of these genes during development can be analysed in vivo after producing targeted mutations. Mutations can be generated by homologous recombination in the genome of embryonic stem cells and can then be introduced into the mouse germ line by means of germ‐line chimaeras. Additional approaches employing stem cells to identify and mutate putative developmental genes are coming into use.

show abstract

Involvement of the Chox-4 chicken homeobox genes in determination of anteroposterior axial polarity during limb development

Cited by 210 publications

References 40 publications

The pattern of expression of the chicken homolog of HOX1I in the developing limb suggests a possible role in the ectodermal inhibition of chondrogenesis

The pattern of expression of the chicken homolog of HOX1I in the developing limb suggests a possible role in the ectodermal inhibition of chondrogenesis

Syndecan‐3, tenascin‐C, and the development of cartilaginous skeletal elements and joints in chick limbs

The molecular and genetic analysis of mouse development

Contact Info

Product

Resources

About