Ionic liquid‐assisted SDS‐PAGE to improve human serum protein separation

Zhang, Tao; Gai, Qingqing; Qu, Feng; Zhang, Yukui

doi:10.1002/elps.201100184

Cited by 9 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SDS-PAGE, which was proposed by Laemmli in 1970, is a charming and powerful tool for protein characterization [ 96 ]. The principle of SDS-PAGE is the positive correlation between electrophoretic mobility of protein and the molecular mass [ 97 – 99 ]. SDS, a kind of anionic detergent, could denature original proteins, eliminate protein’s original surface charge and destroy the structure.…”

Section: Methods To Investigate the Interaction Between Active Herbalmentioning

confidence: 99%

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Jiao

Wang

Jiang

et al. 2018

Chemistry Central Journal

View full text Add to dashboard Cite

Traditional Chinese medicine (TCM), as a unique form of natural medicine, has been used in Chinese traditional therapeutic systems over two thousand years. Active components in Chinese herbal medicine are the material basis for the prevention and treatment of diseases. Research on drug-protein binding is one of the important contents in the study of early stage clinical pharmacokinetics of drugs. Plasma protein binding study has far-reaching influence on the pharmacokinetics and pharmacodynamics of drugs and helps to understand the basic rule of drug effects. It is important to study the binding characteristics of the active components in Chinese herbal medicine with plasma proteins for the medical science and modernization of TCM. This review summarizes the common analytical methods which are used to study the active herbal components-protein binding and gives the examples to illustrate their application. Rules and influence factors of the binding between different types of active herbal components and plasma proteins are summarized in the end. Finally, a suggestion on choosing the suitable technique for different types of active herbal components is provided, and the prospect of the drug-protein binding used in the area of TCM research is also discussed.

show abstract

Section: Methods To Investigate the Interaction Between Active Herbalmentioning

confidence: 99%

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Jiao

Wang

Jiang

et al. 2018

Chemistry Central Journal

View full text Add to dashboard Cite

show abstract

“…Most importantly, ILs with the property of amphiphilicity can promote hydrophobic interactions with proteins. Given that, they have been used as functional molecules fixed with stationary phase or as additives in HPLC and CE for the separation of large bio-molecules [103][104][105]. In addition, TSILs served as the hydrophobic solvent in LLE and as the hydrophilic solvent in ATPS for the extraction of proteins were reported in the last ten years [106][107][108][109][110][111][112][113][114][115].…”

Section: Tsils For the Purification Of Proteins And Peptidesmentioning

confidence: 99%

Recent progress of task‐specific ionic liquids in chiral resolution and extraction of biological samples and metal ions

Cai

Zhao

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

Ionic liquids have been functionalized for modern applications. The functional ionic liquids are also called task-specific ionic liquids. Various task-specific ionic liquids with certain groups have been constructed and exploited widely in the field of separation. To take advantage of their properties in separation science, task-specific ionic liquids are generally used in techniques such as liquid-liquid extraction, solid-phase extraction, gas chromatography, high-performance liquid chromatography, and capillary electrophoresis. This review mainly covers original research papers published in the last five years, and we will focus on task-specific ionic liquids as the chiral selectors in chiral resolution and as extractant or sensor for biological samples and metal ion purification. K E Y W O R D Schiral ionic liquids, chiral resolution, metal extraction, protein purification, task-specific ionic liquids

show abstract

“…An interesting publication on the separation of proteins in human serum described the use of the IL 1-Butyl-3-methylpyrrolidinium bromide ([C 4 mprd][Br]) [84]. In this work, imidazolium-type, pyridinium-type, and pyrrolidium-type ILs with a C 2 -C 8 alkyl chain were employed during the preparation of polyacrylamide gel, and the modified gel was then used for human serum protein separation.…”

Section: Bloodmentioning

confidence: 99%

Bioanalytical separation and preconcentration using ionic liquids

et al. 2013

View full text Add to dashboard Cite

Ionic liquids (ILs) are novel solvents that display a number of unique properties, such as negligible vapor pressure, thermal stability (even at high temperatures), favorable viscosity, and miscibility with water and organic solvents. These properties make them attractive alternatives to environmentally unfriendly solvents that produce volatile organic compounds. In this article, a critical review of state-of-the-art developments in the use of ILs for the separation and preconcentration of bioanalytes in biological samples is presented. Special attention is paid to the determination of various organic and inorganic analytes--including contaminants (e.g., pesticides, nicotine, opioids, gold, arsenic, lead, etc.) and functional biomolecules (e.g., testosterone, vitamin B12, hemoglobin)--in urine, blood, saliva, hair, and nail samples. A brief introduction to modern microextraction techniques based on ILs, such as dispersive liquid-liquid microextraction (DLLME) and single-drop microextraction (SDME), is provided. A comparison of IL-based methods in terms of their limits of detection and environmental compatibilities is also made. Finally, critical issues and challenges that have arisen from the use of ILs in separation and preconcentration techniques are also discussed.

show abstract

Ionic liquid‐assisted SDS‐PAGE to improve human serum protein separation

Cited by 9 publications

References 40 publications

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Recent progress of task‐specific ionic liquids in chiral resolution and extraction of biological samples and metal ions

Bioanalytical separation and preconcentration using ionic liquids

Contact Info

Product

Resources

About