Ionizing Radiation Induces Human Intercellular Adhesion Molecule-1 In Vitro

Behrends, Uta; Peter, Ralf Uwe; Hintermeier-Knabe, R.; Eißner, Günther; Holler, Ernst; Bornkamm, Georg W.; Caughman, S. Wright; Degitz, Klaus

doi:10.1111/1523-1747.ep12398607

Cited by 92 publications

(60 citation statements)

References 27 publications

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“…A 1.8-kb EcoNI/EcoRI fragment of the human ICAM-1 cDNA (2) was radiolabeled with [␣-32 P]dATP (Amersham Biosciences) via random hexamer primer extension and used as a hybridization probe. A 0.21-kb PCR product amplified from a human glycerolaldehyde-3-phosphate dehydrogenase (GAPDH) cDNA was labeled and used as a control probe as described previously (6). Hybridization, stringent washes, and exposure of films to hybridized blots was performed as described previously (28).…”

Section: Methodsmentioning

confidence: 99%

“…It is a glycosylated transmembrane protein of the immunoglobulin superfamily and possesses five extracellular immunoglobulin-like domains (2,3). Although under physiologic conditions ICAM-1 is expressed on only a few cell types including vascular endothelial cells, it is induced on many other cells by cytokine-like tumor necrosis factor-␣, IL-1, or IFN-␥ (4, 5) but also by exogenous inflammatory stimuli (4,6,7). Binding of up-regulated ICAM-1 to its ligands on leukocytes, the integrins LFA-1 (CD11a/CD18) or Mac-1 (CD11b/CD18), is involved in several immunologic events that are pivotal in the establishment of inflammatory responses, especially in leukocyte extravasation, target cell lysis by cytotoxic T cells, or antigen presentation to T cells (1,8).…”

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confidence: 99%

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Specific Inhibition of ICAM-1 Expression Mediated by Gene Targeting with Triplex-forming Oligonucleotides

Besch

Giovannangéli

Kammerbauer

et al. 2002

Journal of Biological Chemistry

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Selected sequences in the DNA double helix can be specifically recognized by oligonucleotides via hydrogen bonding interactions. The resulting triple helix can modulate DNA metabolism and especially interfere with transcription in a gene-specific manner. To explore the potential of triplex-forming oligonucleotides (TFOs) as gene repressors, a TFO was designed to target a 16-bp sequence within the third intron of the human intercellular-adhesion molecule-1 (ICAM-1) gene, which plays a key role in initiating inflammation. TFO binding to its ICAM-1 target sequence was characterized in vitro and also demonstrated in cell nuclei with the set-up of a novel magnetic capture assay, which represents a general experimental approach to the detection of specific TFO binding and to the determination of the accessibility of a given genomic DNA locus. In a human keratinocyte cell line (A431), we observed that: (i) the ICAM-1 target sequence in the chromatin context within the nuclei is still available for triplex formation and (ii) TFO inhibits sequence and gene-specific interferon-␥-induced ICAM-1 surface expression. Collectively, the data demonstrate effective and specific inhibition of ICAM-1 expression by TFO treatment and support the view that triplex-mediated gene targeting might be a valuable technique for anti-inflammatory or anticancer strategies.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Specific Inhibition of ICAM-1 Expression Mediated by Gene Targeting with Triplex-forming Oligonucleotides

Besch

Giovannangéli

Kammerbauer

et al. 2002

Journal of Biological Chemistry

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“…Moist desquamation is characterized by a painful peeling skin with exposure of the dermis, exudate production and ulceration. Moist desquamation is observed in <10% of patients and is dose-dependent (70). Ideally, surviving germinal cells regenerate to repopulate the epidermis and allow for healing.…”

Section: Wound Healing In Irradiated Skinmentioning

confidence: 99%

Radiotherapy and wound healing: Principles, management and prospects (Review)

Gieringer

Gosepath²,

Naim³

2011

Oncol Rep

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Abstract. radiation therapy is a major therapeutic modality in the management of cancer patients. Over 60% of these patients receive radiotherapy at some point during their course of treatment and over 90% will develop skin reactions after therapy. problematic wound healing in radiation-damaged tissue constitutes a major surgical difficulty and despite all efforts, irradiated skin remains a therapeutic challenge. this review provides an overview of the fundamental principles of radiation therapy with regards to the wound healing in normal and irradiated skin. Furthermore, it presents techniques that describe how to prevent and manage skin side effects as well as prospects that may improve cutaneous wound repair in general and in irradiated skin.

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“…The radiation-induced inflammatory response is preceded by the NF-κB transcription factor that up-regulated ICAM-1 expression on endothelial cells [32,33] . These findings provide the rationale for manipulation of NF-κB system as a mean of controlling transcription-dependent cellular events that are involved in radiation-induced inflammatory response.…”

Section: Regulation Of Endothelial Adhesion Molecular Expressionmentioning

confidence: 99%

Radiation-induced intestinal inflammation

Mollà¹,

Panés²

2007

WJG

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Radiation induces an important inflammatory response in the irradiated organs, characterized by leukocyte infiltration and vascular changes that are the main limiting factor in the application of this therapeutic modality for the treatment of cancer. Recently, a considerable investigative effort has been directed at determining the molecular mechanisms by which radiation induces leukocyte recruitment, in order to create strategies to prevent intestinal inflammatory damage. In these review, we consider current available evidence on the factors governing the process of leukocyte recruitment in irradiated organs, mainly derived from experimental studies, with special attention to adhesion molecules, and their value as therapeutic targets.

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Ionizing Radiation Induces Human Intercellular Adhesion Molecule-1 In Vitro

Cited by 92 publications

References 27 publications

Specific Inhibition of ICAM-1 Expression Mediated by Gene Targeting with Triplex-forming Oligonucleotides

Specific Inhibition of ICAM-1 Expression Mediated by Gene Targeting with Triplex-forming Oligonucleotides

Radiotherapy and wound healing: Principles, management and prospects (Review)

Radiation-induced intestinal inflammation

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