2007
DOI: 10.1007/s10534-006-9058-3
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Iron acquisition in the dental pathogen Actinobacillus actinomycetemcomitans: What does it use as a source and how does it get this essential metal?

Abstract: Actinobacillus actinomycetemcomitans requires iron to grow under limiting conditions imposed by synthetic and natural chelators. Although none of the strains tested used hemoglobin, lactoferrin or transferrin, all of them used FeCl3 and hemin as iron sources under chelated conditions. Dot-blot binding assays showed that all strains bind lactoferrin, hemoglobin, and hemin but not transferrin. When compared with smooth strains, the rough isolates showed higher hemin binding activity, which was sensitive to prote… Show more

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Cited by 34 publications
(36 citation statements)
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“…In Escherichia coli, the QseBC two-component signal transduction system has been suggested to regulate biofilm formation (14), and A. actinomycetemcomitans contains an operon that displays 70 to 80% sequence similarity to the QseBC genes of E. coli. QseBC is also similar to the FeuPQ two-component system that regulates iron uptake in Rhizobium leguminosarum, Sinorhizobium meliloti, and Brucella suis (11), and iron acquisition is known to be regulated by AI-2 (13) and dramatically influences biofilm formation (35,36). In this study, we show that qseBC is part of the AI-2 regulon and that its induction requires a functional AI-2 receptor.…”
mentioning
confidence: 64%
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“…In Escherichia coli, the QseBC two-component signal transduction system has been suggested to regulate biofilm formation (14), and A. actinomycetemcomitans contains an operon that displays 70 to 80% sequence similarity to the QseBC genes of E. coli. QseBC is also similar to the FeuPQ two-component system that regulates iron uptake in Rhizobium leguminosarum, Sinorhizobium meliloti, and Brucella suis (11), and iron acquisition is known to be regulated by AI-2 (13) and dramatically influences biofilm formation (35,36). In this study, we show that qseBC is part of the AI-2 regulon and that its induction requires a functional AI-2 receptor.…”
mentioning
confidence: 64%
“…Several operons that encode iron acquisition pathways have been shown to be regulated by AI-2 (13), and iron availability is also known to dramatically influence biofilm formation by A. actinomycetemcomitans (35,36). Thus, it is possible that the biofilm defect that arises from inactivation of qseC occurs in part from the dysregulation of iron acquisition mechanisms.…”
Section: Discussionmentioning
confidence: 99%
“…1 and Table 1). Each plasmid was transformed into E. coli RA1051 (⌬exbBD::aph ⌬tolQR) or E. coli KP1344 (tonB::bla) separately to test its role in iron acquisition, as described for the analysis of the AfeABCD iron transport system of Aggregatibacter actinomycetemcomitans (33). To test whether exbD 1.1 and exbD 1.2 are functional, each exbD component was individually interrupted by inverse PCR using Phusion DNA polymerase (New England BioLabs) with primers 3451 and 3452 (exbD 1.1 ) or primers 3453 and 3454 (exbD 1.2 ) and with pMU672 as the DNA template ( Fig.…”
Section: Methodsmentioning
confidence: 99%
“…on May 12, 2018 by guest http://jb.asm.org/ ExbBD, even facilitating siderophore and iron uptake (15,16,18,20,44,52,109). On the other hand, it is possible that L. pneumophila utilizes a system that is very different from both TonB-ExbBD and TolAQR.…”
Section: Vol 193 2011mentioning
confidence: 99%