Iron nano modulated growth and biosynthesis of steviol glycosides in Stevia rebaudiana

Khan, Mubarak Ali; Ali, Amir; Mohammad, Sher; Ali, Huma; Khan, Tariq; Mashwani, Zia‐ur‐Rehman; Jan, Asif; Ahmad, Parvaiz

doi:10.1007/s11240-020-01902-6

Cited by 34 publications

(16 citation statements)

References 51 publications

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“…Callus induction is an important step to dedifferentiate the mature plant tissue and mediate the process of in vitro morphogenesis for the production of entire intact plantlets [30]. However, in vitro callogenesis is affected by several factors, such as type and concentration of plant growth regulators age and type of explant, growth media composition and physical factors including pH, temperature and light conditions in the growth room [31]. To select a suitable explant and PGRs for the initiation and establishment of callus cultures in S. marianum in the present study, we used three different explants including root, hypocotyl, and cotyledon cut out from the in vitro germinated seedlings and were cultured on MS media supplemented with the combination of 2.4-D and BA at various doses (1.1 mg/L, 2.2 mg/L or 3.3 mg/L).…”

Section: Discussionmentioning

confidence: 99%

Interactive effects of zinc oxide nano particles and different light regimes on growth and silymarin biosynthesis in callus cultures of Silybum marianum L.

Shehzad

Khan

Ali

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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Section: Discussionmentioning

confidence: 99%

Interactive effects of zinc oxide nano particles and different light regimes on growth and silymarin biosynthesis in callus cultures of Silybum marianum L.

Shehzad

Khan

Ali

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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“…Elicitation has been a handy tool for improved secondary metabolites' production in different medicinal plants in vitro cultures. The role of nanoparticles as abiotic elicitors for producing secondary metabolites from the in vitro cultures is a new frontier in metabolic engineering approaches (Khan et al 2020a ). These nanoparticles execute oxidative stress in the plant cells grown via in vitro culture techniques, activating their metabolism and enhancing antioxidant activities (Javed et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Iron-doped zinc oxide nanoparticles-triggered elicitation of important phenolic compounds in cell cultures of Fagonia indica

Khan

et al. 2021

Plant Cell Tiss Organ Cult

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The callus cultures of Fagonia indica could prove as factories for the production of important phytochemicals when triggered through different types of stress. In this study, we initiated callus cultures from healthy stem explants in the presence of iron-doped zinc oxide nanoparticles (Fe-ZnO-NPs). We performed experiments with the callus cultures of F. indica to determine the impact of Fe-ZnO-NPs in concentrations (15.62–250 µg/mL) on biomass accumulation, production of important phenolic and flavonoids, and antioxidative potential. Our results showed that maximum callus biomass [Fresh weight (FW) = 13.6 g and Dry weight (DW) = 0.58 ± 0.01] was produced on day 40 when the media was supplemented with 250 μg/mL Fe-ZnO-NPs. Similarly, maximum total phenolic content (268.36 μg GAE/g of DW) was observed in 40 days old callus added with 125 μg/mL Fe-ZnO-NPs. Maximum total flavonoid content (78.56 μg QE/g of DW) was recorded in 20 days old callus grown in 62.5 μg/mL Fe-ZnO-NPs containing media. Maximum total antioxidant capacity (390.74 µg AAE/g of DW) was recorded in 40 days old callus with 125 μg/mL Fe-ZnO-NPs treated cultures, respectively. Similarly, the highest free radical scavenging activity (93.02%) was observed in callus derived from media having 15.62 µg/mL Fe-ZnO-NPs. The antioxidant potential was observed to have positive correlation with TPC (r = 0.44). HPLC analysis showed that Fe-ZnO-NPs produced compounds (e.g., Epigallocatechin gallate) that were either absent or in lesser quantities in the control group. These results showed that Fe-ZnO-NPs elicitors could increase the biomass and activate secondary metabolism in F. indica cells. Supplementary Information The online version contains supplementary material available at 10.1007/s11240-021-02123-1.

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“…Nanotechnology is a promising field creating innovative nanomaterials, which have a wide range of applications in different fields including agricultural, medicinal and pharmaceutical industries. The role of nanoparticles in vitro might include inducing the growth of cultivated plants in selected growth media (Ha et al 2020;Khan et al 2020 andSotoodehnia-Korani et al 2020), increasing the plantlets resistance or tolerance to stress like drought stress (Mozafari et al 2018), ameliorating the oxidative damage (Pinto et al 2019), but the rooting of plantlets still needs more investigations. No doubt that the applied nanoparticles may cause harmful impact compared to their bulk counterpart as published in case of nickel oxide nanoparticles (Pinto et al 2019), but the biological synthesis of these particles in general might to be less toxicity and safe for the media as reported for nano-Se (Domokos-Szabolcsy et al 2012 and.…”

Section: Discussionmentioning

confidence: 99%

Response of Phalaenopsis Orchid to Selenium and Bio-Nano-Selenium: In Vitro Rooting and Acclimatization

Seliem

Abdalla

El-Ramady

2020

EBSS

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O RCHID plants are considered one of the most valuable and largest flowering plant families, which have a premium position in the global market of cut-flowers. Rooting of orchids is still the main real problem that obstructs its propagation, as roots are hardly to develop.So, Orchid needs to improve its in vitro propagation by enhancing its rooting development. The plant tissue culture of orchids is the most promising field that has been commercially used for getting of virus-free and high-quality rooted plants and representing around 70% of transplants production. In this study the shoots of in vitro plantlets were separated and cultured on half MS medium supplemented with nano-Se (0,10, 20, 30, 40 and 50 mg l-1) or corresponding doses of bulk type selenite; sodium selenite (0, 0.5, 1, 2, 4, 6, 8 and 10 mg l-1) in separates treatments with or without 0.5 mg l-1 NAA (α-naphthalene acetic acid). The two different sources of selenium were selected at mentioned concentrations to improve orchid rooting and acclimatization. Supplementation of half MS medium with 50 mg l-1 nano-Se + 0.5 mg l-1 NAA recorded the highest significant values of rooting percentage and root length as well. Whereas, half MS medium fortified with 40 mg l-1 nano-Se + 0.5 mg l-1 NAA gave the optimum number of roots. On the other hand, this enhancing effect on rooting decreased after augmentation the culture medium with 10 mg l-1 selenite. It could be concluded that in vitro rooting of Phalaenopsis orchid was improved by supplementation of nano-Se up to 50 mg l-1 in presence of 0.5 mg l-1 NAA consequence, acclimatization process was enhanced.

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Iron nano modulated growth and biosynthesis of steviol glycosides in Stevia rebaudiana

Cited by 34 publications

References 51 publications

Interactive effects of zinc oxide nano particles and different light regimes on growth and silymarin biosynthesis in callus cultures of Silybum marianum L.

Interactive effects of zinc oxide nano particles and different light regimes on growth and silymarin biosynthesis in callus cultures of Silybum marianum L.

Iron-doped zinc oxide nanoparticles-triggered elicitation of important phenolic compounds in cell cultures of Fagonia indica

Response of Phalaenopsis Orchid to Selenium and Bio-Nano-Selenium: In Vitro Rooting and Acclimatization

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