Iron Oxide Nanoparticles as Carriers for DOX and Magnetic Hyperthermia after Intratumoral Application into Breast Cancer in Mice: Impact and Future Perspectives

Piehler, Susann; Dähring, Heidi; Grandke, Julia; Göring, Julia; Couleaud, Pierre; Aires, Antonio; Cortajarena, Aitziber L.; Courty, José; Latorre, Alfonso; Somoza, Álvaro; Teichgräber, Ulf; Hilger, Ingrid

doi:10.3390/nano10061016

Cited by 35 publications

(26 citation statements)

References 54 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Langmuir technique was used for the formation of analogs of the biological membrane under laboratory conditions. The conditions similar to the physiological environment were maintained to investigate the hybrid interactions with them [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

Hybrid System for Local Drug Delivery and Magnetic Hyperthermia Based on SPIONs Loaded with Doxorubicin and Epirubicin

et al. 2021

View full text Add to dashboard Cite

Cancer is one of the most common causes of death worldwide, thus new solutions in anticancer therapies are highly sought after. In this work, superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with anticancer drugs are synthesized and investigated as potential magnetic drug nanocarriers for local drug delivery and mild magnetic hyperthermia. We have obtained a hybrid system loaded with holmium and anticancer drugs and thoroughly studied it with respect to the size, morphology, surface modifications and magnetic properties, and interactions with the model of biological membranes, cytotoxicity. We present that nanoparticles having a round shape and size 15 nm are successfully stabilized to avoid their agglomeration and modified with doxorubicin or epirubicin within a controlled way. The number of drugs loaded into the SPIONs was confirmed with thermogravimetry. The hybrid based on SPIONs was investigated in touch with model biological membranes within the Langmuir-Blodgett technique, and results show that modified SPION interacts effectively with them. Results obtained with magnetic hyperthermia and biological studies confirm the promising properties of the hybrid towards future cancer cell treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

Hybrid System for Local Drug Delivery and Magnetic Hyperthermia Based on SPIONs Loaded with Doxorubicin and Epirubicin

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Some studies have reported that local HT using magnetic nanoparticles (MNPs) was effective for cancer treatment. Piehler, S. et al (2020) have reported that local magnetic HT using DOX-functionalized MNPs (43 °C, 1 h) was effective for tumor regression in breast cancer cells (BT474) in vivo [ 20 ]. Thus, local HT combined with chemotherapy may be further developed in the future.…”

Section: Discussionmentioning

confidence: 99%

Elevated Production of Mitochondrial Reactive Oxygen Species via Hyperthermia Enhanced Cytotoxic Effect of Doxorubicin in Human Breast Cancer Cell Lines MDA-MB-453 and MCF-7

Terasaki

Kurokawa

Ito

et al. 2020

IJMS

View full text Add to dashboard Cite

Hyperthermia (HT) treatment is a noninvasive cancer therapy, often used with radiation therapy and chemotherapy. Compared with 37 °C, 42 °C is mild heat stress for cells and produces reactive oxygen species (ROS) from mitochondria. To involve subsequent intracellular accumulation of DOX, we have previously reported that the expression of ATP-binding cassette sub-family G member 2 (ABCG2), an exporter of doxorubicin (DOX), was suppressed by a larger amount of intracellular mitochondrial ROS. We then hypothesized that the additive effect of HT and chemotherapy would be induced by the downregulation of ABCG2 expression via intracellular ROS increase. We used human breast cancer cell lines, MCF-7 and MDA-MB-453, incubated at 37 °C or 42 °C for 1 h to clarify this hypothesis. Intracellular ROS production after HT was detected via electron spin resonance (ESR), and DOX cytotoxicity was calculated. Additionally, ABCG2 expression in whole cells was analyzed using Western blotting. We confirmed that the ESR signal peak with HT became higher than that without HT, indicating that the intracellular ROS level was increased by HT. ABCG2 expression was downregulated by HT, and cells were injured after DOX treatment. DOX cytotoxicity enhancement with HT was considered a result of ABCG2 expression downregulation via the increase of ROS production. HT increased intracellular ROS production and downregulated ABCG2 protein expression, leading to cell damage enhancement via DOX.

show abstract

“…The expressions for the Néel and Brownian relaxation times, as well as the effective relation time, are given by [ 7 , 46 , 49 ]:

where τ 0 = 10 −9 s is the time constant called the attempt time, η (Pa∙s) is the viscosity of the ferrofluid, and V h (m 3 ) is the hydrodynamic volume of the MNP. The parameter ( Γ ) can be defined as [ 34 , 51 ]:

where K denotes the anisotropy constant of a magnetic nanoparticle, and V m (m 3 ) is the volume of its magnetic core, given by the formula:

where R is the radius of the magnetic particle. The hydrodynamic volume is a function of the non-magnetic hydrodynamic layer thickness δ (m) of MNPs and can be determined from [ 7 , 46 ]:

…”

Section: Mathematical Modelingmentioning

confidence: 99%

“…As this, theoretically, does not depend on the product f × H 2 (A 2 /m 2 /s), it seems to be better for comparing various exposure conditions of tested MF samples. The transformation of EM energy is a complex function of the magnetic field strength, electromagnetic field (EMF) frequency, and the concentration of MNPs [ 51 ]. In this regard, Lanier et al [ 52 ] listed different types of MNPs parameters and investigated the correlation between the properties of MNPs and the SLP and ILP values.…”

Section: Introductionmentioning

confidence: 99%

Numerical Investigation of Ferrofluid Preparation during In-Vitro Culture of Cancer Therapy for Magnetic Nanoparticle Hyperthermia

Raouf

Gas

Kim

2021

Sensors

View full text Add to dashboard Cite

Recently, in-vitro studies of magnetic nanoparticle (MNP) hyperthermia have attracted significant attention because of the severity of this cancer therapy for in-vivo culture. Accurate temperature evaluation is one of the key challenges of MNP hyperthermia. Hence, numerical studies play a crucial role in evaluating the thermal behavior of ferrofluids. As a result, the optimum therapeutic conditions can be achieved. The presented research work aims to develop a comprehensive numerical model that directly correlates the MNP hyperthermia parameters to the thermal response of the in-vitro model using optimization through linear response theory (LRT). For that purpose, the ferrofluid solution is evaluated based on various parameters, and the temperature distribution of the system is estimated in space and time. Consequently, the optimum conditions for the ferrofluid preparation are estimated based on experimental and mathematical findings. The reliability of the presented model is evaluated via the correlation analysis between magnetic and calorimetric methods for the specific loss power (SLP) and intrinsic loss power (ILP) calculations. Besides, the presented numerical model is verified with our experimental setup. In summary, the proposed model offers a novel approach to investigate the thermal diffusion of a non-adiabatic ferrofluid sample intended for MNP hyperthermia in cancer treatment.

show abstract

Iron Oxide Nanoparticles as Carriers for DOX and Magnetic Hyperthermia after Intratumoral Application into Breast Cancer in Mice: Impact and Future Perspectives

Cited by 35 publications

References 54 publications

Hybrid System for Local Drug Delivery and Magnetic Hyperthermia Based on SPIONs Loaded with Doxorubicin and Epirubicin

Hybrid System for Local Drug Delivery and Magnetic Hyperthermia Based on SPIONs Loaded with Doxorubicin and Epirubicin

Elevated Production of Mitochondrial Reactive Oxygen Species via Hyperthermia Enhanced Cytotoxic Effect of Doxorubicin in Human Breast Cancer Cell Lines MDA-MB-453 and MCF-7

Numerical Investigation of Ferrofluid Preparation during In-Vitro Culture of Cancer Therapy for Magnetic Nanoparticle Hyperthermia

Contact Info

Product

Resources

About