Iron oxide nanoparticles immobilized Aspergillus flavus manganese peroxidase with improved biocatalytic, kinetic, thermodynamic, and dye degradation potentialities

Kalsoom, Umme; Ahsan, Zainab; Bhatti, Haq Nawaz; Amin, Faiza; Nadeem, Raziya; Aftab, Kiran; Bilal, Muhammad

doi:10.1016/j.procbio.2022.04.002

Cited by 34 publications

(11 citation statements)

References 59 publications

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“…Then enzyme was cooled in an ice bath and put at 4 °C overnight, and the enzyme activity was measured spectrophotometrically. Enzyme retained its activity at 60 °C showing a similar trend to that of peroxidase used by Darwesh (2019), and cabbage waste peroxidase [23].…”

Section: Temperature Effect and Thermal Stabilitysupporting

confidence: 63%

Citrus limon peroxidase-assisted biocatalytic approach for biodegradation of reactive 1847 colfax blue P3R and 621 colfax blue R dyes

Riaz

Kalsoom

Bhatti

et al. 2022

Bioprocess Biosyst Eng

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One of the big environmental problems in today’s world is dye-contaminated toxic waste. Peroxidase is known as highly efficient for the degradation of various pollutants, including dyes. Environmental contamination caused by the discharge of dyes into water bodies is an onerous challenge that poses both human and ecological hazards. In the current studies, biocatalysts used for enzyme decolorization (1847 Colafx Blue P3R and 621 Colafx Blue) are regarded as an eco-friendly method utilizing commonly available low-cost material lemon peels (Citruslimon peroxidase). Peroxidase was extracted in a phosphate buffer of pH 7.0 and partially purified by 20–80% ammonium sulfate precipitation technique from Citruslimon peels. The soluble enzyme was characterized in terms of kinetic and thermodynamic parameters. The values of Km and Vmax (23.16 and 204.08 μmol/ml/min) were determined, respectively. The enzyme showed maximum activity at pH 5.0 and a temperature of 55 °C. Citruslimon efficiently degraded 1847 Colafx Blue P3R and 621 Colafx Blue R dyes with maximum degradation of 83 and 99%, respectively, with an initial dye concentration of 200 ppm at pH 4 and 35 °C temperature within 5–10 min of incubation time. The effect of the redox mediator on the degradation process was examined. Results showed that the peroxidase HOBT system efficiently enhanced the degradation of dyes from water. Hence, Citruslimon peroxidase is an efficient biocatalyst for the treatment of effluents.

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Section: Temperature Effect and Thermal Stabilitysupporting

confidence: 63%

Citrus limon peroxidase-assisted biocatalytic approach for biodegradation of reactive 1847 colfax blue P3R and 621 colfax blue R dyes

Riaz

Kalsoom

Bhatti

et al. 2022

Bioprocess Biosyst Eng

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“…Kalsoom et al. 16 isolated an MnP from a solid-state culture of Aspergillus flavus , showing maximum catalytic potential at 35°C and pH 4.0. The enzyme catalyzed 94% decolorization of Direct red 31 and 85% of Acid black 234, while 100% and 92% of decolorization were achieved with immobilized MnP, respectively, after 24 h. In summary, the MnP extracted from Trametes sp.…”

Section: Enzyme-based Process Biodegradationmentioning

confidence: 99%

“… 13 Also, the use of genetically modified microrganisms (GMOs) can be implemented to overcome any wild strain adversities in the production process, such as stress conditions. 14 In recent years, many works evaluated specific enzymes, such as laccases, 15 peroxidases, 16 , 17 hydrolases, 18 and so on. To the best of our knowledge, since the work of Bilal et al., 3 no work has covered yet the recent innovations in terms of immobilization techniques and genetic engineering in the field of enzyme-based processes for EC bioremediation, with the perspective on how it could be incorporated in worldwide wastewater treatment plants.…”

Section: Introductionmentioning

confidence: 99%

Emerging contaminants bioremediation by enzyme and nanozyme-based processes – A review

et al. 2023

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Section: Enzyme‐incorporated Nanomaterials In Various Applicationsmentioning

confidence: 99%

“…Kalsoom and coworkers (2022) developed iron oxide NPs immobilized with manganese peroxidase to study the efficiency in the removal of acid black 234 and direct red 31 dye. The study showed after 24 h, the immobilized manganese peroxidase on iron oxide NPs showed 92% and 100% decolorization, respectively (Kalsoom et al, 2022). Lignin peroxidase immobilized on graphene oxide functionalized MnFe 2 O 4 NPs showed excellent removal of nigrosine, bromophenol blue, coomassie brilliant blue, and methylene blue at a decolorization percentage of 57.2%, 81.6%, 51.4%, and 88.2% within 1 h, respectively (Rathour et al, 2020).…”

Section: Enzyme‐incorporated Nanomaterials In Various Applicationsmentioning

confidence: 99%

Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications

Anboo

Lau

Kansedo

et al. 2022

Biotech & Bioengineering

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Over the past decade, nanotechnology has been developed and employed across various entities. Among the numerous nanostructured material types, enzymeincorporated nanomaterials have shown great potential in various fields, as an alternative to biologically derived as well as synthetically developed hybrid structures. The mechanism of incorporating enzyme onto a nanostructure depends on several factors including the method of immobilization, type of nanomaterial, as well as operational and environmental conditions. The prospects of enzymeincorporated nanomaterials have shown promising results across various applications, such as biocatalysts, biosensors, drug therapy, and wastewater treatment. This is due to their excellent ability to exhibit chemical and physical properties such as high surface-to-volume ratio, recovery and/or reusability rates, sensitivity, response scale, and stable catalytic activity across wide operating conditions. In this review, the evolution of enzyme-incorporated nanomaterials along with their impact on our society due to its state-of-the-art properties, and its significance across different industrial applications are discussed. In addition, the weakness and future prospects of enzyme-incorporated nanomaterials were also discussed to guide scientists for futuristic research and development in this field.

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Iron oxide nanoparticles immobilized Aspergillus flavus manganese peroxidase with improved biocatalytic, kinetic, thermodynamic, and dye degradation potentialities

Cited by 34 publications

References 59 publications

Citrus limon peroxidase-assisted biocatalytic approach for biodegradation of reactive 1847 colfax blue P3R and 621 colfax blue R dyes

Citrus limon peroxidase-assisted biocatalytic approach for biodegradation of reactive 1847 colfax blue P3R and 621 colfax blue R dyes

Emerging contaminants bioremediation by enzyme and nanozyme-based processes – A review

Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications

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