Is cellulose sponge degradable or stable as implantation material? An in vivo subcutaneous study in the rat

Märtson, Matis; Viljanto, J; Hurme, Timo; Laippala, Pékka; Saukko, Pekka

doi:10.1016/s0142-9612(99)00094-0

Cited by 207 publications

(142 citation statements)

References 11 publications

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“…A material composed of porous plant cellulose has been shown to be biocompatible with bone tissue and hepatocytes. 9,20 Research conducted on an implanted cellulose sponge showed that it can be regarded as a slowly degradable material. 9 As mentioned by the same authors, this material can be considered nondegradable if used as a temporary wound coverage for a short period of time.…”

Section: The Significant Biomedical Potential Of Microbial Cellulose mentioning

confidence: 99%

The Future Prospects of Microbial Cellulose in Biomedical Applications

et al. 2006

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Microbial cellulose has proven to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, such as paper products, electronics, acoustics, and biomedical devices. In fact, biomedical devices recently have gained a significant amount of attention because of an increased interest in tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. Due to its unique nanostructure and properties, microbial cellulose is a natural candidate for numerous medical and tissue-engineered applications. For example, a microbial cellulose membrane has been successfully used as a wound-healing device for severely damaged skin and as a small-diameter blood vessel replacement. The nonwoven ribbons of microbial cellulose microfibrils closely resemble the structure of native extracellullar matrices, suggesting that it could function as a scaffold for the production of many tissue-engineered constructs. In addition, microbial cellulose membranes, having a unique nanostructure, could have many other uses in wound healing and regenerative medicine, such as guided tissue regeneration (GTR), periodontal treatments, or as a replacement for dura mater (a membrane that surrounds brain tissue). In effect, microbial cellulose could function as a scaffold material for the regeneration of a wide variety of tissues, showing that it could eventually become an excellent platform technology for medicine. If microbial cellulose can be successfully mass produced, it will eventually become a vital biomaterial and will be used in the creation of a wide variety of medical devices and consumer products.

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Section: The Significant Biomedical Potential Of Microbial Cellulose mentioning

confidence: 99%

The Future Prospects of Microbial Cellulose in Biomedical Applications

et al. 2006

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“…Cellulose is degraded in nature by fungal and microbial enzymes through hydrolase attack on the b(1-4) linkages [27]. However, these enzymes are absent in the mammals.…”

Section: Cell Studymentioning

confidence: 99%

“…However, these enzymes are absent in the mammals. Consequently, a study in a rat model reported that cellulose sponges did not fully degrade after 60 weeks in vivo [27]. In addition, bacterial cellulose has a high degree of crystallinity, which should retard its degradation in vitro and in vivo.…”

Section: Cell Studymentioning

confidence: 99%

Microporous bacterial cellulose as a potential scaffold for bone regeneration

et al. 2010

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a b s t r a c tNanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 lm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.

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“…However, cellulose in the human organism behaves as a non-degradable or very slowly degradable material. For example, the degradation time of viscose cellulose sponges implanted subcutaneously into rats was longer than 60 weeks (Märtson et al 1999). This very slow degradability of cellulose is due to the absence of enzymes that attack the b(1 ?…”

Section: Introductionmentioning

confidence: 99%

“…This very slow degradability of cellulose is due to the absence of enzymes that attack the b(1 ? 4) linkage (these enzymes are present in microbial and fungal cells; for a review, see Märtson et al 1999). An efficient method for inducing degradability of cellulose is its oxidation.…”

Section: Introductionmentioning

confidence: 99%

Cellulose-based materials as scaffolds for tissue engineering

et al. 2013

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Two types of cellulose-based materials, 6-carboxycellulose with 2.1 or 6.6 wt% of -COOH groups, were prepared and tested for potential use in tissue engineering. The materials were functionalized with arginine, i.e. an amino acid with a basic side chain, or with chitosan, in order to balance the relatively acid character of oxidized cellulose molecules, and were seeded with vascular smooth muscle cells (VSMC). The cell adhesion and growth were then evaluated directly on the materials, and also on the underlying polystyrene culture dishes. Of these two types of studied materials, 6-carboxycellulose with 2.1 wt% of -COOH groups was more appropriate for cell colonization. The cells on this material achieved an elongated shape, while they were spherical in shape on the other materials. The number of cells and the concentration (per mg of protein) of contractile proteins alpha-actin and SM1 and SM2 myosins, i.e. markers of the phenotypic maturation of VSMC, were also significantly higher on this material. Functionalization of the material with arginine and chitosan further improved the phenotypic maturation of VSMC. Chitosan also improved the adhesion and growth of these cells. In comparison with the control polystyrene dishes, the proliferation of cells on our cellulose-based materials was relatively low. This suggests that these materials can be used in applications where high proliferation activity of cells is not desirable, e.g. proliferation of VSMC on vascular prostheses. Alternatively, the cell proliferation might be enhanced by another more efficient modification, which would require further research.

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Is cellulose sponge degradable or stable as implantation material? An in vivo subcutaneous study in the rat

Cited by 207 publications

References 11 publications

The Future Prospects of Microbial Cellulose in Biomedical Applications

The Future Prospects of Microbial Cellulose in Biomedical Applications

Microporous bacterial cellulose as a potential scaffold for bone regeneration

Cellulose-based materials as scaffolds for tissue engineering

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