Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

Priolli, Denise Gonçalves; Silva, Pamela Lícia Eiras da; Betini, Adriane Moro; Pereira, José Aires; Margarido, Nelson Fontana

doi:10.1590/s0102-86502009000600011

Cited by 3 publications

(4 citation statements)

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“…The collagen content of the Peyer’s plaque is lower compared to other parts of the colon. The collagen amount can affect the healing process of the anastomosis line 32 . We did not take into consideration the location of the Peyer’s plaque while performing the resection and end-to-end anastomosis.…”

Section: Discussionmentioning

confidence: 99%

Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury

et al. 2020

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Purpose To examine the effects of quercetin on healing of experimental colon anastomosis injury in early and late period. Methods Eighty male Wistar-Albino rats were divided into 8 groups. For all groups, left colons of the rats were resected and for the rest end-to-end anastomosis was performed. Two of the groups for which the experiment protocol was ended on the 3rd and 7th day following the anastomosis were not administered with either quercetin or dimethylsulfoxide DMSO, whereas two other groups were administered with DMSO only, and four other groups were administered with quercetin dissolved in DMSO in doses of 20 and 100 mg/kg during the protocol. At the end of the study, anastomosis line was resected, histopathological evaluation was performed and bursting pressure, malondialdehyde, superoxide dismutase, catalase, and hydroxyproline levels were measured. Results Quercetin significantly increased hydroxyproline, superoxide dismutase, catalase levels, histopathological healing score, bursting pressure values and decreased malondialdehyde level in early period. It also significantly increased superoxide dismutase, catalase, and hydroxyproline levels and decreased malondialdehyde level in late period. Conclusion It was seen that quercetin speeds up the injury healing process and reveals an antioxidant effect, specifically in early period.

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Section: Discussionmentioning

confidence: 99%

Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury

et al. 2020

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“…In the Ischemia and HBOT/Ischemia groups, ischemia was induced as follows: Peyer's patch and the peritoneal reflection were identified 15 as the landmark for the procedure. A colon segment 2 cm cranial to the landmark was isolated and the venous-arterial branches that drained or irrigated this left colonic segment were electrocoagulated.…”

Section: Anesthesia and Surgical Proceduresmentioning

confidence: 99%

Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

Fontoura-Andrade

Pinto²,

Carneiro

et al. 2020

Acta Cir. Bras.

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“…Estudos que não identificam exatamente o local de onde foram removidos os espécimes para análise histológica, identificando o local de remoção do mesmo apenas com expressões do tipo "reto alto", "cólon distal" ou "acima da reflexão peritoneal", podem ter comparados segmentos cólicos obtidos de locais distintos, onde estas variáveis podem sofrer alterações75 . Foi por isso que adotamos a placa de Peyer como reparo anatômico para a coleta do fragmento cólico, como proposto anteriormente76 . Assim, excluíamos da análise histológica a placa de Peyer, estrutura linfoide que poderia interferir na avaliação da presença de infiltrado inflamatório e na altura das criptas, pois neste local a população de células inflamatórias é significativamente maior e a altura das criptas, menor.…”

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“…Assim, excluíamos da análise histológica a placa de Peyer, estrutura linfoide que poderia interferir na avaliação da presença de infiltrado inflamatório e na altura das criptas, pois neste local a população de células inflamatórias é significativamente maior e a altura das criptas, menor. Utilizando a placa de Peyer como reparo anatômico, tínhamos a certeza que os segmentos distais do cólon a serem analisados foram removidos em todos os animais, independente do grupo experimental a que pertenciam, sempre de um mesmo local.Esse cuidado, a nosso ver, tornou as aferições deste estudo mais fidedignas.O mesmo pode ser dito em relação aos trabalhos que não tiveram o cuidado de remover os resíduos fecais que costumam permanecer no cólon excluso durante a realização da derivação do trânsito intestinal76 . A presença de resíduos fecais nestes segmentos poderia fornecer ainda certa quantidade de AGCC para as células da mucosa exclusa, reduzindo a intensidade do processo inflamatório e promovendo um trofismo epitelial.…”

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Avaliação dos efeitos da terapia biológica com infliximabe na colite de exclusão

Godoy Delben

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Introduction: Diversion colitis (DC) is a chronic inflammatory process that develops in the mucosa of the colonic segments devoid of fecal stream. The increase in the production of reactive oxygen species and inflammatory cytokines in these segments suggests that the DC has etiopathogenic molecular mechanisms like what occurs in IBD. While the use of anti-TNF-α antibodies, particularly infliximab, represents one of the most effective therapeutic strategies for the treatment of IBD, little is known about the effect of infliximab in the treatment of DC, human or experimental. Objective: To evaluate the effects of infliximab therapy on inflammation and tissue content of TNFα in the colic mucosa of rats, with or without fecal stream. Method: Twenty-two male Wistar rats underwent intestinal diversion by making a proximal terminal colostomy and closing of the distal stump (Hartmann procedure). The animals remained for 12 weeks with intestinal derivation for the development of DC in the excluded segment.Then, they were divided into three experimental groups, as submitted, weekly, to subcutaneous application of saline (2ml/week) or infliximab at the dosages of 5mg/kg /week and 10mg/kg/week, for five consecutive weeks. After this period, the animals were euthanized and segments of the colon, with and without fecal stream, were removed. Histopathological changes and the intensity of the inflammatory process were assessed by histological study hematoxylin-eosin staining. The inflammatory tissue grade was assessed considering the intensity of epithelial atrophy, epithelial loss, presence of abscesses in the colonic glands, leukocyte infiltrate, and number of goblet cells, being stratified according to a previously validated scale. The tissue content of TNF-α in colic segments was evaluated by its tissue expression, identified by immunohistochemical study. Results: Segments without fecal stream had a higher inflammatory score when compared to segments with fecal stream, regardless of the intervention substance, as well as the infliximab dosage used. The infliximab intervention significantly reduced the inflammatory score in the segments without fecal stream. Higher tissue content of TNF-α was found in segments without fecal stream, regardless of the experimental group considered. The application of infliximab did not modify the tissue content of TNF-α, in segments without intestinal stream, regardless of the dosage used. Conclusions: In the experimental DC model used, the subcutaneous application of infliximab decreased the inflammatory process in the mucosa devoid of fecal stream, despite not reducing the tissue content of TNFα.

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Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

Cited by 3 publications

References 14 publications

Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury

Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury

Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

Avaliação dos efeitos da terapia biológica com infliximabe na colite de exclusão

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