Is the red fluorescence of dental plaque related to its cariogenicity?

Bittar, Daniela Gonçalves; Pontes, Laura Regina Antunes; Calvo, Ana Flávia Bissoto; Novaes, Tatiane Fernandes; Braga, Mariana Minatel; Freitas, Patrícia Moreira de; Tabchoury, Cínthia Pereira Machado; Mendes, Fausto Medeiros

doi:10.1117/1.jbo.19.6.065004

Cited by 20 publications

(16 citation statements)

References 21 publications

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“…Similar to other previous studies, 11,12,15 we observed that the R/ G fluorescence ratio was related to biofilm maturation, although the value did not change after six days. In addition, the R/G ratio was strongly correlated with green value rather than red value.…”

Section: Discussionsupporting

confidence: 80%

“…[8][9][10][11] Although some researchers have demonstrated that the red fluorescence emitted by dental plaque is not an essential factor in cariogenicity, red fluorescence of biofilm indicates that these biofilms were mature. 12,13 When we observed plaque with QLF, it has been found that biofilms emitting red fluorescence were primarily composed of anaerobic bacteria that are related not only to caries but also to periodontitis. 13 Moreover, some studies have suggested that increases in red fluorescence according to the maturation of the biofilm are due to ecological changes in the biofilm and not increases in specific species.…”

Section: Introductionmentioning

confidence: 99%

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Ecological changes in oral microcosm biofilm during maturation

et al. 2016

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Abstract. The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Ecological changes in oral microcosm biofilm during maturation

et al. 2016

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“…1 Research is currently being undertaken to correlate plaque fluorescence with the progression of oral diseases, such as dental caries. [2][3][4][5] The three possible mechanisms by which changes in antecedent growth conditions and the local physicochemical environment (i.e., pH) could affect plaque's fluorescent emissions are as follows.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the effect of local pH on fluorescence emissions from oral bacteria of the genusPrevotella

Hope

Higham

2016

J. Biomed. Opt

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Abstract. A number of anaerobic oral bacteria, notably Prevotellaceae, exhibit red fluorescence when excited by short-wavelength visible light due to their accumulation of porphyrins, particularly protoporphyrin IX. pH affects the fluorescence of abiotic preparations of porphyrins due to transformations in speciation between monomers, higher aggregates, and dimers. To elucidate whether the porphyrin speciation phenomenon could be manifested within a microbiological system, suspensions of Prevotella intermedia and Prevotella nigrescens were examined by fluorescence spectrophotometry while being titrated against NaOH. The initial pH of the samples was <6, which was then raised toward the maximum found within a diseased periodontal pocket, being ∼pH 8.7. The intensity of the fluorescence emissions increased between 600 and 650 nm with increasing pH. Peak fluorescence emissions occurred at 635 AE 1 nm with a second emission peak developing with increasing pH at 622 nm. A linear relationship was demonstrated between pH and the log 10 ratio of 635:622 nm excitation fluorescence intensities. These findings suggest that the pH range found within the oral cavity could affect the fluorescence of oral bacteria in vivo, which may in turn have connotations for any clinical diagnoses that may be inferred from dental plaque fluorescence. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Moreover, an objective method could be less influenced by examiner's experience than would visual inspection. 4 Considering that LFpen and QLF (red fluorescence measurement) are methods based on the fluorescence emitted by microorganisms present in the caries lesions, 9,11,15 and since active caries lesions are more infected than inactive ones, 16 this possibility would be plausible. The old version of the LF method used with different drying times had been tested for this purpose previously, 28 but this methodology was not compared with visual inspection.…”

Section: Discussionmentioning

confidence: 99%

“…14,15 Thus, assessment of red fluorescence could be an adjunctive method for caries activity assessment, since active lesions are more infected than inactive ones. 16,17 To the best of our knowledge, however, no previous study was carried out to evaluate the accuracy of QLF in detecting occlusal caries lesions, nor for the assessment of caries activity status in primary teeth.…”

Section: Introductionmentioning

confidence: 99%

Clinical performance of fluorescence-based methods for detection of occlusal caries lesions in primary teeth

et al. 2017

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We aimed to investigate the performance of fluorescencebased methods (FBMs), compared to visual inspection after histological validation, in detecting and assessing the activity status of occlusal carious lesions in primary teeth. One examiner evaluated 50 primary molars close to exfoliation in 24 children. Teeth were assessed using quantitative light-induced fluorescence (QLF) and pen-type laser fluorescence (LFpen). After exfoliation, histological validation was performed. Teeth were cut and sections were evaluated for lesion depth and activity status (after utilization of a pH indicator) under a stereomicroscope. Parameters related to the performance of the methods in detecting caries lesions at two thresholds (initial and dentin lesions) were calculated. Regarding the activity status, lesions were classified into sound+inactive or active, and the area under the ROC curve and the diagnostic odds ratio values of the methods were calculated and compared. Evaluation of red fluorescence using QLF presented higher sensitivity but lower specificity than visual inspection in detecting dentin caries lesions. However, QLF considering different parameters and LFpen had similar performance to that obtained with visual inspection. Regarding activity assessment, all FBMs and visual inspection also presented similar performance. In conclusion, FBMs did not prove advantageous for the detection and activity assessment of occlusal caries lesions in primary molars when compared to visual inspection.

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Is the red fluorescence of dental plaque related to its cariogenicity?

Cited by 20 publications

References 21 publications

Ecological changes in oral microcosm biofilm during maturation

Ecological changes in oral microcosm biofilm during maturation

Evaluating the effect of local pH on fluorescence emissions from oral bacteria of the genusPrevotella

Clinical performance of fluorescence-based methods for detection of occlusal caries lesions in primary teeth

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