Isocratic high-performance liquid chromatographic method for quantitative determination of lysine, histidine and tyrosine in foods

Sanz, Martial; Castillo, G. Del; Hernández, Adolfo

doi:10.1016/0021-9673(95)00232-4

Cited by 26 publications

(13 citation statements)

References 18 publications

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“…The concentration of BSA in the microspheres was determined by the Lowry protein assay 19. Lysine concentration in the TPP and different media was determined by reversed‐phase high‐performance liquid chromatography (HPLC) following precolumn derivitization with 5‐dimethylaminonaphthalene‐1‐sulfonyl chloride 20. The derivitized samples and standards were filtered (0.45 μm) and then injected (10 μL) with an autosampler into a Hewlett–Packard (Burlington, MA) Model 1050 HPLC, using a Spherisorb ODS column (Phenomenex; Torrance, CA) at ambient temperature.…”

Section: Methodsmentioning

confidence: 99%

Effect of preparation method on the capture and release of biologically active molecules in chitosan gel beads

Ghanem

Skonberg

2002

J of Applied Polymer Sci

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Model bioactive compounds with different molecular and functional characteristics were entrapped in gel beads prepared by polyelectrolyte complexation of chitosan and pentasodium tripolyphosphate (TPP). Three compounds of interest to the food and biomedical industries were tested: (1) lysine, (2) bovine serum albumin (BSA), and (3) ␤-galactosidase. Effects of the compound concentration in the initial chitosan solution, pH of the curing solution, and length of the curing phase on the capture efficiency were evaluated. Release rates for lysine and BSA into a phosphate buffer, distilled water, and a synthetic ocean solution were observed, and the activity of the entrapped ␤-galactosidase was determined. The capture efficiencies for lysine and BSA decreased as the concentration increased. The capture efficiency for lysine ranged from 90% at pH 5 to 20% at pH 8.6. There was no significant effect of the release media on the rate of release. BSA and lysine release reached 90% of the maximum after 100 and 50 min, respectively. The capture efficiency of ␤-galactosidase was not affected by the pH of TPP; however, enzyme activity in the beads decreased as the pH increased. Beads prepared in the pH 8.6 TPP solution had significantly higher rates of enzyme release over time.

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Section: Methodsmentioning

confidence: 99%

Effect of preparation method on the capture and release of biologically active molecules in chitosan gel beads

Ghanem

Skonberg

2002

J of Applied Polymer Sci

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“…Determination of biological active amines and amino acid from pharmaceutical preparations continues to attract the attention of analytical chemists. A number of different derivatizing reagents are reported for their determination using UV or fluorimetric detection [33][34][35][36][37][38][39] . The procedures are based on the determination of a number of amines and amino acids simultaneously or the determination of a limited number of the analytes from specific matrix.…”

Section: Resultsmentioning

confidence: 99%

Determination of Dopamine, Glycine, Tryptohan, Valine, Leucine and Isoleucine Using 4-Dimethylaminobenzaldehyde by HPLC in Pharmaceutical Samples

Panjwani¹,

Arain²,

Khuhawar³

2014

Asian J. Chem.

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A simple isocratic HPLC procedure has been developed for simultaneous separation and quantitation of dopamine, glycine, tryptophan, valine, leucine and isolucine by pre-column derivatization with 4-dimethylaminobenzaldehyde and UV detection at 240 nm. The derivatives formed were examined spectrophotomatrically for the effects of pH, derivatizing reagent concentration and heating time in aqueousmethanolic solution. The analytes and excess of the derivatizing reagent separated completely from the column Kromasil 100 C-18 5 µm, (250 × 0.46 cm) when eluted with methanol-water-potassium chloride (1M) adjusted to pH 2 (55:44.75:0.25 v/v/v) with a flow rate of 0.4 mL min -1 . Linear calibration were obtained with 2-400 µg mL -1 and limits of detection within 13.2-533.2 ng/20 µL. A number of amino acids and pharmaceutical additives did not interfere to the determination. The derivatization, separation and quantitation was repeatable with RSD within 4.5 (n = 6). The method was used for determination of dopamine, tryptophan, valine, leucine and isoleucine from pharmaceutical preparation. The results were further supported by standard addition method. The recovery from pharmaceutical preparation of dopamine was 96.8 %, tryptophan 99.6 %, valine 98.7 %, leucine 98.8 % and isoleucine 99.3 % with RSD (n = 3) 3.4, 3.1, 1.3, 3.8 and 1.8 %, respectively. The results were further supported by standard addition method.

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“…Dansylation reaction medium must be also carefully selected from the point of view of solubility of all compounds of interest or else product yield would suffer. Various solvents= buffers=mixtures most commonly reported in the literature include: NaHCO 3 =Na 2 CO 3 =K 2 CO 3 , [40] boronic buffer, [41] NaOH=NaHCO 3 solution, [42] saturated NaHCO 3 solution, [43] saturated Na 2 CO 3 solution, [44] the 40 mM lithium carbonate, [45] and triethylamine. [46] Unfortunately, none of the aforementioned solvents was satisfactory for the purpose of this study due to low solubility of at least one of the studied compounds.…”

Section: Determination Of Free Amino Acids 671mentioning

confidence: 99%

Simultaneous Determination of Free Amino Acids, L-Carnosine, Purine, Pyrimidine, and Nucleosides in Meat by Liquid Chromatography/Single Quadrupole Mass Spectrometry

Szterk

Roszko

2014

Journal of Liquid Chromatography & Related Technologies

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& A novel approach to single-run determination of 25 free amino acids, L-carnosine, 4 nitrogen bases (purine and pyrimidine), and 5 nucleosides in unpurified biological samples is reported. The analytes were extracted from the sample, derivatized with dansyl chloride, and analyzed using RP-HPLC-DAD-ESI-MS. The reported method features high sensitivity (LOQs in 5-10 ng mL À1 range), wide linearity range with r > 0.94, and high precision (intra-day RSD within the 0.1-4.2% range). Analyte average recovery coefficient was in the 70.1-111.3% range. The method was used to determine levels of free amino acids, L-carnosine, nitrogen bases, and nucleosides in beef (strip loin). Evolution of concentrations of the studied compounds during meat storage processes (vacuum packing, cold storage) was also investigated.

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Isocratic high-performance liquid chromatographic method for quantitative determination of lysine, histidine and tyrosine in foods

Cited by 26 publications

References 18 publications

Effect of preparation method on the capture and release of biologically active molecules in chitosan gel beads

Effect of preparation method on the capture and release of biologically active molecules in chitosan gel beads

Determination of Dopamine, Glycine, Tryptohan, Valine, Leucine and Isoleucine Using 4-Dimethylaminobenzaldehyde by HPLC in Pharmaceutical Samples

Simultaneous Determination of Free Amino Acids, L-Carnosine, Purine, Pyrimidine, and Nucleosides in Meat by Liquid Chromatography/Single Quadrupole Mass Spectrometry

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