Isoenzyme Expression During Root and Shoot Formation in Solanum Nigrum

Hassanein, Anber M. A.; Ahmed, Ambreen; Abed-El-Hafez, A. I. I.; Soltan, D. M.

doi:10.1023/a:1002452900035

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…Esterases are proteins which hydrolyze ester bonds and are present in several isoenzyme forms. Esterases have hydrolytic action on broad range of substrates and they could be used as a molecular marker for somatic embryogenesis and organogenesis (Hassanein et al, 1999). Under the influence of biotic and abiotic stresses, variation in esterase expression was registered in peanut (Hassanein, 1999) in barley (Tamás et al, 2005) and tomato (Hassanein, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In this concern, appearance or disappearance of isoenzyme gives an indication about how gene expression is influenced by specific agent. In addition, change in staining intensity of one or more bands indicates to change in the enzyme activity (Khavkin and Zabordina, 1994;Hassanein et al, 1999). Also, this indicates that genes involved in expression of these isoenzyme forms are differentially expressed under the influence of studied biotic or abiotic condition (Chawla, 1991;Hassanein et al, 1999;El-Tayeb and Hassanein,2000).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, change in staining intensity of one or more bands indicates to change in the enzyme activity (Khavkin and Zabordina, 1994;Hassanein et al, 1999). Also, this indicates that genes involved in expression of these isoenzyme forms are differentially expressed under the influence of studied biotic or abiotic condition (Chawla, 1991;Hassanein et al, 1999;El-Tayeb and Hassanein,2000). Comparative studies on esterase isoenzyme expression under different abiotic stresses in oil producing plants were limited.…”

Section: Introductionmentioning

confidence: 99%

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Differential responses of esterase isoenzyme of peanut to salinity and drought as influenced by salicylic acid

Soltan

Salem

2017

Journal of Environmental Studies

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential responses of esterase isoenzyme of peanut to salinity and drought as influenced by salicylic acid

Soltan

Salem

2017

Journal of Environmental Studies

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“…Presence or absence of isoenzyme form shows a change in the isoenzyme expression. In addition, an increase in the staining intensity of the detected band represents an increase in the activity of the studied isoenzyme (22,23) indicating that the gene involved in the synthesis of this isoenzyme form is differentially activated under the studied agent (24,23,25). This work was fulfilled to determine the suitable growth regulator combination, which resulted in efficient in vitro shoot multiplication and alteration in gene expression, and how they were influenced by the osmotic potential of culture medium using different media and different concentrations of mannitol and NaCl.…”

Section: Introductionmentioning

confidence: 99%

IMPROVEMENT OF IN VITRO PROPAGATION OF JOJOBA BY LOW CONCENTRATIONS OF NaCl OR MANNITOL

2016

Assiut University Journal of Multidisciplinary Scientific Resea

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This work was done to improve our understanding about the effect of growth regulators on micropropagation and gene expression of stress tolerant jojoba plant and how they were influenced by the presence of different stress agents. Genetic variation of plants obtained from seeds of jojoba, was checked using RAPD technique. The registered genetic variation was too high and to avoid it, offspring one shoot was used in this study. A combination of 3 mg/l BAP and 0.1 mg/l NAA was the best for jojoba micropropagation, while changing the concentrations from that decreased shoot multiplication and growth. Application of GA 3 is not recommended for in vitro multiplication of jojoba. In case of jojoba as stress tolerant plant species, the effect of the selected growth regulators combination (3 mg/l BAP + 0.1 mg/l NAA) on micropropagation was improved when high salt-MS medium containing low concentrations of NaCl or mannitol was used. The negative effect of NaCl or mannitol on in vitro shoot growth was only detected at concentration 4 g/l. On the other side, lead acetate abolished the effect of growth regulators on jojoba micropropagation even in low concentration. Isoenzymes and SDS-PAGEs indicated that the registered effects of growth regulators and stress agents on shoot multiplication and growth were associated with variation in gene expression.

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Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrida microshoots

Kotis¹,

Yupsanis²,

Syros³

et al. 2009

Biologia plant.

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Specific activities and isoform patterns of peroxidases, acid phosphatases, DNases and RNases were studied in relation to in vitro rooting of Petunia × hybrida microshoots in the presence of 4 μM indole-3-butyric acid (IBA). Specific activities of the above enzymes increased in the course of rooting. Rhizogenesis could be related with an increased specific activity of peroxidases during the initiation phase, in parallel with increased lignin content. Twelve peroxidases, six anionic (A1 -A6) and six cationic (C1 -C6), seven acid phosphatases (ACP1 -ACP7), seven RNases (R1 -R7) and four DNases (D1 -D4) isoforms were detected following native PAGE. Variation in the number of the above isoforms and their quantity was observed during different stages of rooting. Particularly, A2, A3, C3, C4, C5, ACP2, R1, R2, R3, and D4 isoforms appeared after the induction phase and could be related to emergence of root primordia. Additionally, R3 and D4 could be associated with cell division and differentiation, since these are only expressed in rooted microshoots. Moreover, the higher number of roots in IBA-treated microshoots could be related to the higher expression of RNase and DNase isoforms during initiation and expression phases.

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Isoenzyme Expression During Root and Shoot Formation in Solanum Nigrum

Cited by 8 publications

References 16 publications

Differential responses of esterase isoenzyme of peanut to salinity and drought as influenced by salicylic acid

Differential responses of esterase isoenzyme of peanut to salinity and drought as influenced by salicylic acid

IMPROVEMENT OF IN VITRO PROPAGATION OF JOJOBA BY LOW CONCENTRATIONS OF NaCl OR MANNITOL

Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrida microshoots

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